Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0003389
UniProt IDQ7Z3T8
Primary gene name(s)ZFYVE16
Synonym gene name(s)KIAA0305
Protein nameZinc finger FYVE domain-containing protein 16
Protein functionMay be involved in regulating membrane trafficking in the endosomal pathway. Overexpression induces endosome aggregation. Required to target TOM1 to endosomes. {ECO:0000269|PubMed:11546807, ECO:0000269|PubMed:14613930}.
Subcellular locationCytoplasm {ECO:0000269|PubMed:11546807}. Early endosome membrane {ECO:0000269|PubMed:11546807};
Peripheral membrane protein {ECO:0000269|PubMed:11546807}. Note=Localized to early endosomes. Membrane-associated, probably via its association with phosphatidylinositol 3-phosphate, PI3P.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: Q7Z3T8
Gene Ontology
(Biological Process)
Complete annatation
BMP signaling pathway [GO:0030509];
endosomal transport [GO:0016197];
protein targeting to lysosome [GO:0006622];
regulation of endocytosis [GO:0030100];
signal transduction [GO:0007165];
vesicle organization [GO:0016050]
Gene Ontology
(Molecular Function)
Complete annatation
1-phosphatidylinositol binding [GO:0005545];
metal ion binding [GO:0046872];
phosphatidylinositol-3,4,5-trisphosphate binding [GO:0005547];
protein transporter activity [GO:0008565]
Gene Ontology
(Cellular Component)
Complete annatation
cytoplasm [GO:0005737];
early endosome [GO:0005769];
early endosome membrane [GO:0031901];
intracellular membrane-bounded organelle [GO:0043231]
Protein-protein interaction115111
Phylogenetic treeQ7Z3T8
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD3-0.3667716043714640.2653344510400850.379861392533281
AZA vs. DISU0.1912875659152540.5331408438609460.940949166421897
AZA vs. IL7-0.2783296924413470.1546677523027360.922020841096286
AZA vs. SAHA0.389140526739310.1141894609569560.431652741093196
DISU vs. CD30.5443133094543830.1378344068892880.239893297477097
DISU vs. IL7-0.4774119771726240.1071553005504230.428706516931428
DISU vs. SAHA0.1983165736967750.5201774442633270.836223387999211
DMSO vs. AZA-0.07110403050220420.6775411883387241
DMSO vs. CD30.2826706006351420.3791559381697760.493475957540187
DMSO vs. DISU-0.2645817469985050.3488172978293540.842312150921815
DMSO vs. IL7-0.1999415794486070.2747296579015930.769860685669313
DMSO vs. SAHA0.4525624512080750.05729272920991150.273078223421937
HIV vs. Mock in Activation-0.08173880273155880.8965140646373960.999983755607037
HIV vs. Mock in Latency-0.1085196768214460.517059135844650.999834320637052
IL7 vs. CD30.09820674520509610.7609800294436410.840892373461408
SAHA vs. CD30.7298169726435550.04077666621497610.0837489395350561
SAHA vs. IL70.6621373322705320.007258787840776050.0501813137333484
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) TRUE
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK 0.158086 0.407165
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
-0.014 0.006

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
1.019 1.471 1.928 2.136 1.881
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

not found

Protein Secondary Structure       (annotations from PDB)      top

PDB Accession Method Resolution Chain Structure Preview
3T7L X-ray 1.0Å A=733-820.

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

not found

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
hsa04144 Endocytosis - Homo sapiens (human)
hsa04350 TGF-beta signaling pathway - Homo sapiens (human)