Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0003355
UniProt IDP63104
Primary gene name(s)YWHAZ
Synonym gene name(s)unknown
Protein name14-3-3 protein zeta/delta
Protein functionAdapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. {ECO:0000269|PubMed:14578935, ECO:0000269|PubMed:15071501, ECO:0000269|PubMed:15644438, ECO:0000269|PubMed:16376338, ECO:0000269|PubMed:9360956}.
Subcellular locationCytoplasm {ECO:0000269|PubMed:17081065}. Melanosome {ECO:0000269|PubMed:17081065}. Note=Located to stage I to stage IV melanosomes.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: P63104
Gene Ontology
(Biological Process)
Complete annatation
establishment of Golgi localization [GO:0051683];
Golgi reassembly [GO:0090168];
membrane organization [GO:0061024];
negative regulation of apoptotic process [GO:0043066];
platelet activation [GO:0030168];
positive regulation of protein insertion into mitochondrial membrane involved in apoptotic signaling pathway [GO:1900740];
protein targeting [GO:0006605];
regulation of mRNA stability [GO:0043488];
signal transduction [GO:0007165]
Gene Ontology
(Molecular Function)
Complete annatation
identical protein binding [GO:0042802];
poly(A RNA binding [GO:0044822];
protein kinase binding [GO:0019901];
transcription factor binding [GO:0008134];
ubiquitin protein ligase binding [GO:0031625]
Gene Ontology
(Cellular Component)
Complete annatation
blood microparticle [GO:0072562];
cytoplasm [GO:0005737];
cytoplasmic vesicle membrane [GO:0030659];
cytosol [GO:0005829];
extracellular exosome [GO:0070062];
extracellular space [GO:0005615];
focal adhesion [GO:0005925];
melanosome [GO:0042470];
mitochondrion [GO:0005739];
nucleoplasm [GO:0005654];
nucleus [GO:0005634]
Protein-protein interaction113366
Phylogenetic treeP63104
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD30.09802937115882580.7642702301488060.836838424177465
AZA vs. DISU0.08138506363921590.7469749996708410.976008954784031
AZA vs. IL70.1594352408878440.4050551951540880.999311006273513
AZA vs. SAHA-0.3422561585047690.1599043698801650.510569470784496
DISU vs. CD3-0.02922815318199990.9356781380174720.957975934370147
DISU vs. IL70.06889559288589260.7840129405428230.950465331549835
DISU vs. SAHA-0.422362455019490.1473451934150130.493158549112645
DMSO vs. AZA-0.03680137536645690.8251712067994241
DMSO vs. CD3-0.1452520287390130.649104039154620.738576461470117
DMSO vs. DISU-0.1197711779940070.6226039069313360.943804523903158
DMSO vs. IL70.2033205806934380.2562239204243060.759019526361721
DMSO vs. SAHA-0.3123139590073550.1843792426647040.518144195617107
HIV vs. Mock in Activation-0.1055074285645590.8651983264099510.999983755607037
HIV vs. Mock in Latency0.08922727174869080.586776111329450.999834320637052
IL7 vs. CD30.06919583750897040.8290662990100950.889099122902471
SAHA vs. CD3-0.4647348572582730.1882888720570590.287984705358761
SAHA vs. IL7-0.5049562777141820.03800011096534770.152604682542484
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
-0.3804 0.04203

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) FALSE
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK 0.094822 0.601463
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
1.044 1.01 1.115 1.132 1.068
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

not found

Protein Secondary Structure       (annotations from PDB)      top

PDB Accession Method Resolution Chain Structure Preview
1IB1 X-ray 2.7Å A/B/C/D=1-245.
1QJA X-ray 2.0Å A/B=1-245.
1QJB X-ray 2.0Å A/B=1-245.
2C1J X-ray 2.6Å A/B=1-245.
2C1N X-ray 2.0Å A/B=1-245.
2O02 X-ray 1.5Å A/B=1-230.
2WH0 X-ray 2.2Å A/B/C/D=1-245.
3CU8 X-ray 2.4Å A/B=1-245.
3NKX X-ray 2.4Å A/B=1-245.
3RDH X-ray 2.3Å A/B/C/D=1-245.
4BG6 X-ray 2.3Å A/B=1-245.
4FJ3 X-ray 1.9Å A/B=1-230.
4HKC X-ray 2.2Å A=1-245.
4IHL X-ray 2.2Å A/B=1-230.
4N7G X-ray 2.2Å A=1-230.
4N7Y X-ray 2.1Å A/B=1-230.
4N84 X-ray 2.5Å A/B=1-230.
4WRQ X-ray 2.4Å A/B=1-245.
4ZDR X-ray 2.9Å A/B=1-230.
5D2D X-ray 2.1Å A/B=1-230.
5D3F X-ray 2.7Å A/B=1-230.
5EWZ X-ray 2.3Å A/B=1-230.
5EXA X-ray 1.9Å A/B=1-230.
5J31 X-ray 2.4Å A/B=1-230.

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

HIV Partner Interaction Type PubMed
Vpr interacts with 24282540
Vpr enhanced by 11531413
integrase interacts with 22190034
Vpr inhibits 15265780
Tat upregulates 23364796

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
hsa04110 Cell cycle - Homo sapiens (human)
hsa04114 Oocyte meiosis - Homo sapiens (human)
hsa04151 PI3K-Akt signaling pathway - Homo sapiens (human)
hsa04390 Hippo signaling pathway - Homo sapiens (human)
hsa05130 Pathogenic Escherichia coli infection - Homo sapiens (human)
hsa05161 Hepatitis B - Homo sapiens (human)
hsa05169 Epstein-Barr virus infection - Homo sapiens (human)
hsa05203 Viral carcinogenesis - Homo sapiens (human)