Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0003352
UniProt IDP62258
Primary gene name(s)YWHAE
Synonym gene name(s)unknown
Protein name14-3-3 protein epsilon
Protein functionAdapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner.
Subcellular locationCytoplasm {ECO:0000250}. Melanosome {ECO:0000269|PubMed:12042314, ECO:0000269|PubMed:17081065}. Note=Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: P62258
Gene Ontology
(Biological Process)
Complete annatation
cerebral cortex development [GO:0021987];
G2/M transition of mitotic cell cycle [GO:0000086];
hippocampus development [GO:0021766];
hippo signaling [GO:0035329];
intracellular signal transduction [GO:0035556];
membrane organization [GO:0061024];
membrane repolarization during cardiac muscle cell action potential [GO:0086013];
negative regulation of cysteine-type endopeptidase activity involved in apoptotic process [GO:0043154];
negative regulation of peptidyl-serine dephosphorylation [GO:1902309];
neuron migration [GO:0001764];
positive regulation of protein insertion into mitochondrial membrane involved in apoptotic signaling pathway [GO:1900740];
protein targeting [GO:0006605];
regulation of cellular response to heat [GO:1900034];
regulation of heart rate by cardiac conduction [GO:0086091];
regulation of heart rate by hormone [GO:0003064];
regulation of membrane repolarization [GO:0060306];
regulation of potassium ion transmembrane transporter activity [GO:1901016];
substantia nigra development [GO:0021762];
viral process [GO:0016032]
Gene Ontology
(Molecular Function)
Complete annatation
enzyme binding [GO:0019899];
histone deacetylase binding [GO:0042826];
ion channel binding [GO:0044325];
MHC class II protein complex binding [GO:0023026];
phosphoprotein binding [GO:0051219];
phosphoserine binding [GO:0050815];
poly(A RNA binding [GO:0044822];
potassium channel regulator activity [GO:0015459];
protein heterodimerization activity [GO:0046982];
ubiquitin protein ligase binding [GO:0031625]
Gene Ontology
(Cellular Component)
Complete annatation
axon [GO:0030424];
cytoplasmic vesicle membrane [GO:0030659];
cytosol [GO:0005829];
extracellular exosome [GO:0070062];
focal adhesion [GO:0005925];
kinesin complex [GO:0005871];
melanosome [GO:0042470];
membrane [GO:0016020];
mitochondrion [GO:0005739]
Protein-protein interaction113363
Phylogenetic treeP62258
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
      unknown
Brass et al., Science, 2008
      unknown
Smith et al., J. Immunol, 2010
      unknown
Interferon-stimulated
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model


DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD32.016616733564372.7871761565379e-093.64655547147041e-08
AZA vs. DISU-0.1624544911495130.5209597078644810.940509006088293
AZA vs. IL70.4535049234137830.01849782430827680.43977556119662
AZA vs. SAHA-0.3586357671139240.1422021142114350.479971936771869
DISU vs. CD3-2.190889116937856.82844991661824e-091.10560745481314e-07
DISU vs. IL70.6066592196540530.01640584847930490.158131819581536
DISU vs. SAHA-0.1954333656120950.5023974109901380.826869753181416
DMSO vs. AZA-0.05125340174319020.7598102565640611
DMSO vs. CD3-2.078081053054233.88447829458016e-105.46912761755609e-09
DMSO vs. DISU0.1093446782888280.65417841442580.950002654032543
DMSO vs. IL70.5120606057331120.004481682976153150.135123561771727
DMSO vs. SAHA-0.3148413816426930.1822949235469070.51564692056596
HIV vs. Mock in Activation0.002788254624012680.9964188911851910.999983755607037
HIV vs. Mock in Latency-0.2649804031575210.1924993901530970.999834320637052
IL7 vs. CD3-1.555242544773282.12844168967496e-061.92515271693643e-05
SAHA vs. CD3-2.400462051884791.01148311948407e-102.16520605264559e-09
SAHA vs. IL7-0.8151376177896140.0008790395005074950.0105920395129097
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) FALSE
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK 0.184468 0.200502
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
1.032 0.934 1.017 0.964 0.927
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
210996_s_at 1.64 No upregulated in CD8+ cells

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

Drugbank ID Drug Name Drug Status Pharmacological Action Drug Action
DB01780 Fusicoccin experimental unknown unknown

Protein Secondary Structure       (annotations from PDB)      top

PDB Accession Method Resolution Chain Structure Preview
2BR9 X-ray 1.7Å A=1-233.
3UAL X-ray 1.8Å A=1-232.
3UBW X-ray 1.9Å A=1-234.

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

HIV Partner Interaction Type PubMed
Vpr enhanced by 11531413
15142377
15265780
16968670
20012529
integrase interacts with 22190034
Envelope surface glycoprotein gp120 downregulates 11208609
retropepsin cleaves 22944692
Vpr inhibits 15265780
15708996
20012529

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
hsa04110 Cell cycle - Homo sapiens (human)
hsa04114 Oocyte meiosis - Homo sapiens (human)
hsa04151 PI3K-Akt signaling pathway - Homo sapiens (human)
hsa04390 Hippo signaling pathway - Homo sapiens (human)
hsa04722 Neurotrophin signaling pathway - Homo sapiens (human)
hsa05169 Epstein-Barr virus infection - Homo sapiens (human)
hsa05203 Viral carcinogenesis - Homo sapiens (human)
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