Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0003351
UniProt IDP31946
Primary gene name(s)YWHAB
Synonym gene name(s)unknown
Protein name14-3-3 protein beta/alpha
Protein functionAdapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. Negative regulator of osteogenesis. Blocks the nuclear translocation of the phosphorylated form, by AKT1 of SRPK2 and antagonizes its stimulatory effect on cyclin D1 expression resulting in blockage of neuronal apoptosis elicited by SRPK2. Negative regulator of signaling cascades that mediate activation of MAP kinases via AKAP13. {ECO:0000269|PubMed:17717073, ECO:0000269|PubMed:19592491, ECO:0000269|PubMed:21224381}.
Subcellular locationCytoplasm {ECO:0000269|PubMed:17081065}. Melanosome {ECO:0000269|PubMed:17081065}. Note=Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: P31946
Gene Ontology
(Biological Process)
Complete annatation
cytoplasmic sequestering of protein [GO:0051220];
hippo signaling [GO:0035329];
MAPK cascade [GO:0000165];
membrane organization [GO:0061024];
negative regulation of G-protein coupled receptor protein signaling pathway [GO:0045744];
negative regulation of protein dephosphorylation [GO:0035308];
negative regulation of transcription, DNA-templated [GO:0045892];
positive regulation of catalytic activity [GO:0043085];
positive regulation of protein insertion into mitochondrial membrane involved in apoptotic signaling pathway [GO:1900740];
protein heterooligomerization [GO:0051291];
protein targeting [GO:0006605];
regulation of mRNA stability [GO:0043488];
viral process [GO:0016032]
Gene Ontology
(Molecular Function)
Complete annatation
enzyme binding [GO:0019899];
histone deacetylase binding [GO:0042826];
phosphoprotein binding [GO:0051219];
phosphoserine binding [GO:0050815];
protein domain specific binding [GO:0019904];
transcription corepressor activity [GO:0003714]
Gene Ontology
(Cellular Component)
Complete annatation
cytoplasm [GO:0005737];
cytoplasmic vesicle membrane [GO:0030659];
cytosol [GO:0005829];
extracellular exosome [GO:0070062];
focal adhesion [GO:0005925];
melanosome [GO:0042470];
membrane [GO:0016020];
mitochondrion [GO:0005739];
nucleus [GO:0005634];
perinuclear region of cytoplasm [GO:0048471];
transcriptional repressor complex [GO:0017053]
Protein-protein interaction113361
Phylogenetic treeP31946
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD30.1223364093330040.7083276293906430.792637136367315
AZA vs. DISU-0.1054702055810490.6759576224214690.96445964444266
AZA vs. IL70.1261073189318630.510406472814170.999311006273513
AZA vs. SAHA-0.2332975711278640.3381192199932580.710059782972746
DISU vs. CD3-0.2395758375633980.5080865722370320.632914386406166
DISU vs. IL70.2221785452444460.3767902241843790.747752038345041
DISU vs. SAHA-0.1262718709148760.6644063523638280.897853183854195
DMSO vs. AZA-0.02688451419593750.8719218362568611
DMSO vs. CD3-0.1596961931420320.6170395558690630.710843535150832
DMSO vs. DISU0.07712428903573440.7513239376130810.969857060964769
DMSO vs. IL70.1600203346538460.3718236100704140.830194188375728
DMSO vs. SAHA-0.2127482482427910.3660563988984380.714157223675448
HIV vs. Mock in Activation-0.06972894607893840.9106585111243870.999983755607037
HIV vs. Mock in Latency0.03959503542505950.8097028775727150.999834320637052
IL7 vs. CD30.01079958237249790.9731251517032150.984688198954254
SAHA vs. CD3-0.3795906467888270.2836026331827560.39709392418156
SAHA vs. IL7-0.3619994995475630.1371411696869340.347673392509391
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) FALSE
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK -0.393613 0.00199466
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
0.999 1.044 1.146 1.061 1
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

not found

Protein Secondary Structure       (annotations from PDB)      top

PDB Accession Method Resolution Chain Structure Preview
2BQ0 X-ray 2.5Å A/B=2-239.
2C23 X-ray 2.6Å A=2-239.
4DNK X-ray 2.2Å A/B=1-246.

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

HIV Partner Interaction Type PubMed
Vpr enhanced by 11531413
integrase interacts with 22190034
Vpr inhibits 15265780
Envelope surface glycoprotein gp120 upregulates 15103018

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
hsa04110 Cell cycle - Homo sapiens (human)
hsa04114 Oocyte meiosis - Homo sapiens (human)
hsa04151 PI3K-Akt signaling pathway - Homo sapiens (human)
hsa04390 Hippo signaling pathway - Homo sapiens (human)
hsa05161 Hepatitis B - Homo sapiens (human)
hsa05169 Epstein-Barr virus infection - Homo sapiens (human)
hsa05203 Viral carcinogenesis - Homo sapiens (human)