Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0003319
UniProt IDP30291
Primary gene name(s)WEE1
Synonym gene name(s)unknown
Protein nameWee1-like protein kinase
Protein functionActs as a negative regulator of entry into mitosis, G2 to M transition by protecting the nucleus from cytoplasmically activated cyclin B1-complexed CDK1 before the onset of mitosis by mediating phosphorylation of CDK1 on 'Tyr-15'. Specifically phosphorylates and inactivates cyclin B1-complexed CDK1 reaching a maximum during G2 phase and a minimum as cells enter M phase. Phosphorylation of cyclin B1-CDK1 occurs exclusively on 'Tyr-15' and phosphorylation of monomeric CDK1 does not occur. Its activity increases during S and G2 phases and decreases at M phase when it is hyperphosphorylated. A correlated decrease in protein level occurs at M/G1 phase, probably due to its degradation.
Subcellular locationNucleus.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: P30291
Gene Ontology
(Biological Process)
Complete annatation
cell division [GO:0051301];
establishment of cell polarity [GO:0030010];
G2/M transition of mitotic cell cycle [GO:0000086];
microtubule cytoskeleton organization [GO:0000226];
mitotic nuclear division [GO:0007067];
neuron projection morphogenesis [GO:0048812];
regulation of cell cycle [GO:0051726]
Gene Ontology
(Molecular Function)
Complete annatation
ATP binding [GO:0005524];
kinase activity [GO:0016301];
magnesium ion binding [GO:0000287];
non-membrane spanning protein tyrosine kinase activity [GO:0004715];
protein tyrosine kinase activity [GO:0004713]
Gene Ontology
(Cellular Component)
Complete annatation
cytoplasm [GO:0005737];
nucleolus [GO:0005730];
nucleoplasm [GO:0005654];
nucleus [GO:0005634]
Protein-protein interaction113303
Phylogenetic treeP30291
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD31.739405242438094.88553906186073e-063.23954442324527e-05
AZA vs. DISU0.1195562706830420.6426323110838530.960057478314726
AZA vs. IL70.7170186235030470.002512794385432950.14853078341816
AZA vs. SAHA-0.008674661812263840.9720723847903150.992834220866143
DISU vs. CD3-1.633069527338942.47588505517538e-050.000165212444983239
DISU vs. IL70.5892800998419520.02631597387437280.207197690254735
DISU vs. SAHA-0.1272087308021510.6671511801618070.898778531886885
DMSO vs. AZA-0.03080650981496030.8589592974494941
DMSO vs. CD3-1.783218353451111.56216469793247e-061.07756461421028e-05
DMSO vs. DISU-0.1525972848891610.5389210765505290.921334463652713
DMSO vs. IL70.7551914903465260.0007776768068952220.0482447648722036
DMSO vs. SAHA0.0152001427895850.9493647112134860.988044718092566
HIV vs. Mock in Activation-0.1654380990149620.8432598092273890.999983755607037
HIV vs. Mock in Latency-0.08668854727061410.7001302624137210.999834320637052
IL7 vs. CD3-1.01317128859010.01496623547942020.0415290992969994
SAHA vs. CD3-1.773803594343644.36460063268918e-063.02272663302509e-05
SAHA vs. IL7-0.7297340960715080.003432093757061550.0293450829801167
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) FALSE
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK 0.471761 0.000305467
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
0.889 1.009 0.776 0.852 0.839
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

Drugbank ID Drug Name Drug Status Pharmacological Action Drug Action
DB04608 9-HYDROXY-4-PHENYL-6H-PYRROLO[3,4-C]CARBAZOLE-1,3-DIONE experimental unknown unknown
DB07006 9-HYDROXY-6-(3-HYDROXYPROPYL)-4-(2-METHOXYPHENYL)PYRROLO[3,4-C]CARBAZOLE-1,3(2H,6H)-DIONE experimental unknown unknown
DB07226 N-[4-(2-CHLOROPHENYL)-1,3-DIOXO-1,2,3,6-TETRAHYDROPYRROLO[3,4-C]CARBAZOL-9-YL]FORMAMIDE experimental unknown unknown
DB07257 4-(2-chlorophenyl)-8-(2-hydroxyethyl)-6-methylpyrrolo[3,4-e]indole-1,3(2H,6H)-dione experimental unknown unknown
DB07265 3-(9-HYDROXY-1,3-DIOXO-4-PHENYL-2,3-DIHYDROPYRROLO[3,4-C]CARBAZOL-6(1H)-YL)PROPANOIC ACID experimental unknown unknown
DB08365 8-bromo-4-(2-chlorophenyl)-N-(2-hydroxyethyl)-6-methyl-1,3-dioxo-1,2,3,6-tetrahydropyrrolo[3,4-e]indole-7-carboxamide experimental unknown unknown

Protein Secondary Structure       (annotations from PDB)      top

PDB Accession Method Resolution Chain Structure Preview
1X8B X-ray 1.8Å A=291-575.
2IN6 X-ray 1.9Å A=291-575.
2IO6 X-ray 2.2Å A=291-575.
2Z2W X-ray 2.2Å A=291-575.
3BI6 X-ray 2.2Å A=291-575.
3BIZ X-ray 2.2Å A=291-575.
3CQE X-ray 2.5Å A=291-575.
3CR0 X-ray 2.3Å A=291-575.

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
hsa04110 Cell cycle - Homo sapiens (human)