Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0003291
UniProt IDO00401
Primary gene name(s)WASL
Synonym gene name(s)unknown
Protein nameNeural Wiskott-Aldrich syndrome protein
Protein functionRegulates actin polymerization by stimulating the actin-nucleating activity of the Arp2/3 complex. Involved in mitosis and cytokinesis, via its role in the regulation of actin polymerization. Binds to HSF1/HSTF1 and forms a complex on heat shock promoter elements, HSE that negatively regulates HSP90 expression. {ECO:0000269|PubMed:19366662, ECO:0000269|PubMed:19487689, ECO:0000269|PubMed:22847007, ECO:0000269|PubMed:22921828}.
Subcellular locationNucleus {ECO:0000250|UniProtKB:Q91YD9}. Cytoplasm {ECO:0000250|UniProtKB:Q91YD9}. Cytoplasm, cytoskeleton {ECO:0000250|UniProtKB:O08816}. Note=Preferentially localized in the cytoplasm when phosphorylated and in the nucleus when unphosphorylated. Exported from the nucleus by an nuclear export signal, NES-dependent mechanism to the cytoplasm. {ECO:0000250|UniProtKB:Q91YD9}.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: O00401
Gene Ontology
(Biological Process)
Complete annatation
actin polymerization or depolymerization [GO:0008154];
cell division [GO:0051301];
cellular protein complex localization [GO:0034629];
ephrin receptor signaling pathway [GO:0048013];
Fc-gamma receptor signaling pathway involved in phagocytosis [GO:0038096];
membrane budding [GO:0006900];
mitotic nuclear division [GO:0007067];
movement of cell or subcellular component [GO:0006928];
negative regulation of lymphocyte migration [GO:2000402];
negative regulation of membrane tubulation [GO:1903526];
positive regulation of Arp2/3 complex-mediated actin nucleation [GO:2000601];
positive regulation of clathrin-dependent endocytosis [GO:2000370];
positive regulation of filopodium assembly [GO:0051491];
protein complex assembly [GO:0006461];
regulation of nitric-oxide synthase activity [GO:0050999];
regulation of protein localization [GO:0032880];
regulation of transcription, DNA-templated [GO:0006355];
response to bacterium [GO:0009617];
spindle localization [GO:0051653];
transcription, DNA-templated [GO:0006351];
vesicle organization [GO:0016050];
vesicle transport along actin filament [GO:0030050]
Gene Ontology
(Molecular Function)
Complete annatation
GTPase regulator activity [GO:0030695]
Gene Ontology
(Cellular Component)
Complete annatation
actin cap [GO:0030478];
actin cytoskeleton [GO:0015629];
cytoplasmic, membrane-bounded vesicle [GO:0016023];
cytosol [GO:0005829];
endocytic vesicle membrane [GO:0030666];
extracellular exosome [GO:0070062];
Golgi membrane [GO:0000139];
lamellipodium [GO:0030027];
nucleus [GO:0005634];
plasma membrane [GO:0005886]
Protein-protein interaction114466
Phylogenetic treeO00401
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD3-0.3946072209722220.2332596406487440.343396103131263
AZA vs. DISU0.4415679673632590.1209039731348070.680293576347176
AZA vs. IL70.1426284614285530.4982001732089340.999311006273513
AZA vs. SAHA0.287165160228380.248197415200310.618832313522503
DISU vs. CD30.8231609336794930.02556187995644190.0628375372575677
DISU vs. IL7-0.3061209654865120.3083145892474030.692113911201217
DISU vs. SAHA-0.153647219222820.6455242432084670.890425673072991
DMSO vs. AZA-0.1087794362810180.5314234306523051
DMSO vs. CD30.2740711742804320.3966875485347860.511302012207097
DMSO vs. DISU-0.552085423580490.03647471415844510.385370771162924
DMSO vs. IL70.2583806300505290.1619977238407670.66823629784962
DMSO vs. SAHA0.3881733107711330.1058122303580610.386031499328575
HIV vs. Mock in Activation-0.2352627595096440.708749676971740.999983755607037
HIV vs. Mock in Latency-0.08596156966254810.6125629068595910.999834320637052
IL7 vs. CD30.5476336664616660.09197318517246840.177455085361459
SAHA vs. CD30.6559785191123360.0685058024813840.128533670741342
SAHA vs. IL70.139942728286250.5985846243481130.796086758120879
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) TRUE
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK -0.0209067 0.932167
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
unknown unknown unknown unknown unknown
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

not found

Protein Secondary Structure       (annotations from PDB)      top

PDB Accession Method Resolution Chain Structure Preview
2FF3 X-ray 2.0Å C=451-465.
2LNH NMR - A=207-270.
2VCP X-ray 3.2Å D/E=392-484.
4CC2 X-ray 1.5Å B/D=346-357.
4CC7 X-ray 1.9Å B/D/F/H/J/L/N=346-357.

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

HIV Partner Interaction Type PubMed
Nef inhibits 16687395

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
hsa04062 Chemokine signaling pathway - Homo sapiens (human)
hsa04144 Endocytosis - Homo sapiens (human)
hsa04520 Adherens junction - Homo sapiens (human)
hsa04530 Tight junction - Homo sapiens (human)
hsa04666 Fc gamma R-mediated phagocytosis - Homo sapiens (human)
hsa04810 Regulation of actin cytoskeleton - Homo sapiens (human)
hsa05100 Bacterial invasion of epithelial cells - Homo sapiens (human)
hsa05130 Pathogenic Escherichia coli infection - Homo sapiens (human)
hsa05131 Shigellosis - Homo sapiens (human)
hsa05132 Salmonella infection - Homo sapiens (human)
hsa05231 Choline metabolism in cancer - Homo sapiens (human)