Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0003251
UniProt IDQ53GS9
Primary gene name(s)USP39
Synonym gene name(s)unknown
Protein nameU4/U6.U5 tri-snRNP-associated protein 2
Protein functionPlays a role in pre-mRNA splicing as a component of the U4/U6-U5 tri-snRNP, one of the building blocks of the spliceosome. Regulates AURKB mRNA levels, and thereby plays a role in cytokinesis and in the spindle checkpoint. Does not have ubiquitin-specific peptidase activity, but could be a competitor of ubiquitin C-terminal hydrolases, UCHs. {ECO:0000269|PubMed:11350945, ECO:0000269|PubMed:18728397}.
Subcellular locationNucleus {ECO:0000269|PubMed:11350945}.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: Q53GS9
Gene Ontology
(Biological Process)
Complete annatation
cell cycle [GO:0007049];
cell division [GO:0051301];
mRNA processing [GO:0006397];
mRNA splicing, via spliceosome [GO:0000398];
protein deubiquitination [GO:0016579];
RNA splicing [GO:0008380];
spliceosomal complex assembly [GO:0000245]
Gene Ontology
(Molecular Function)
Complete annatation
thiol-dependent ubiquitinyl hydrolase activity [GO:0036459];
zinc ion binding [GO:0008270]
Gene Ontology
(Cellular Component)
Complete annatation
nucleoplasm [GO:0005654];
spliceosomal complex [GO:0005681]
Protein-protein interaction115939
Phylogenetic treeQ53GS9
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
      Yes - Two siRNA pools inhibit HIV replication and inhibition of Tat-mediated transactivation of the HIV LTR is not observed
Brass et al., Science, 2008
      unknown
Smith et al., J. Immunol, 2010
      unknown
Interferon-stimulated
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model


DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD30.6879810264289270.03662684937236180.0769286334643586
AZA vs. DISU0.1875786431204790.4588935940532460.923299791345253
AZA vs. IL70.1045647321687670.5874920163406920.999311006273513
AZA vs. SAHA0.06508612386083070.7905237627846630.944804652816143
DISU vs. CD3-0.5131129749234120.1575901215018810.265779951477246
DISU vs. IL7-0.09216138327930770.7145905800497340.927165784358565
DISU vs. SAHA-0.1205496615867730.6807917498613290.904604884565563
DMSO vs. AZA-0.03026934483496210.8572012512363731
DMSO vs. CD3-0.7295373568572660.02336298796648910.0509722539775753
DMSO vs. DISU-0.2196557228769750.368693069950350.85177548964527
DMSO vs. IL70.1420814000385410.4307799670784130.858220445510856
DMSO vs. SAHA0.08922501974319050.7061721873206780.911894760281734
HIV vs. Mock in Activation0.0003211803590508880.9995878420688740.999983755607037
HIV vs. Mock in Latency0.04344122458136190.7933443008431720.999834320637052
IL7 vs. CD3-0.576328700450220.0736778910182210.149608898197177
SAHA vs. CD3-0.6469133650374010.07045432249905880.1315345193336
SAHA vs. IL7-0.04214770908374320.8633360117348370.943448386319482
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) FALSE
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK 0.050633 0.858489
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
1.004 0.976 0.965 0.92 0.915
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

not found

Protein Secondary Structure       (annotations from PDB)      top

PDB Accession Method Resolution Chain Structure Preview
3JCR EM 7.0Å V=1-565.

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

HIV Partner Interaction Type PubMed
Rev interacts with 22174317
HIV-1 virus replication enhanced by expression of human gene 18976975

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
hsa03040 Spliceosome - Homo sapiens (human)
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