Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0003224
UniProt IDQ9BZX2
Primary gene name(s)UCK2
Synonym gene name(s)UMPK
Protein nameUridine-cytidine kinase 2
Protein functionPhosphorylates uridine and cytidine to uridine monophosphate and cytidine monophosphate. Does not phosphorylate deoxyribonucleosides or purine ribonucleosides. Can use ATP or GTP as a phosphate donor. Can also phosphorylate cytidine and uridine nucleoside analogs such as 6-azauridine, 5-fluorouridine, 4-thiouridine, 5-bromouridine, N(4-acetylcytidine, N(4-benzoylcytidine, 5-fluorocytidine, 2-thiocytidine, 5-methylcytidine, and N(4-anisoylcytidine.
Subcellular locationunknown
ECO codeClick here for more information.
Amino acid sequence
FASTA format: Q9BZX2
Gene Ontology
(Biological Process)
Complete annatation
CMP salvage [GO:0006238];
CTP salvage [GO:0044211];
pyrimidine nucleobase metabolic process [GO:0006206];
pyrimidine nucleoside salvage [GO:0043097];
UMP salvage [GO:0044206]
Gene Ontology
(Molecular Function)
Complete annatation
ATP binding [GO:0005524];
nucleoside kinase activity [GO:0019206];
uridine kinase activity [GO:0004849]
Gene Ontology
(Cellular Component)
Complete annatation
cytosol [GO:0005829]
Protein-protein interaction113217
Phylogenetic treeQ9BZX2
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD32.654502544987142.53130849614536e-147.86636735132017e-13
AZA vs. DISU-0.4193345886386210.1021987032533140.644788874295266
AZA vs. IL70.6971797647717310.0003771601538616890.0397547902038967
AZA vs. SAHA0.1701444251551630.4911636058651780.815396497225958
DISU vs. CD3-3.084930555499983.77475828372553e-152.03945184117845e-13
DISU vs. IL71.106937110927691.65192878575082e-050.00129024886876669
DISU vs. SAHA0.5911411685200320.04530221817850390.264549150725233
DMSO vs. AZA-0.005557376095489890.9742707741980341
DMSO vs. CD3-2.669312920548514.44089209850063e-151.36534774416147e-13
DMSO vs. DISU0.4124105014651060.09564358624058140.561569786929451
DMSO vs. IL70.7099109132132610.0001094188459778160.0126306951077765
DMSO vs. SAHA0.1690349112000530.4786729517583530.802254515314841
HIV vs. Mock in Activation0.1231365252955760.84304206061490.999983755607037
HIV vs. Mock in Latency0.1467489961299250.3896684226525980.999834320637052
IL7 vs. CD3-1.950962164531074.54041371167335e-097.24207998688386e-08
SAHA vs. CD3-2.508367760502112.00839345154691e-114.89749018039028e-10
SAHA vs. IL7-0.5295129597420010.0315646285634910.135171934933541
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander 0.773 7.52E-07 1.37E-05
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) FALSE
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK 0.808551 0.00830635
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
0.87 1.157 0.835 0.813 1.036
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

Drugbank ID Drug Name Drug Status Pharmacological Action Drug Action
DB02097 Cytidine experimental unknown unknown
DB02431 Cytidine-5&,39;-Triphosphate experimental unknown unknown
DB03403 Cytidine-5&,39;-Monophosphate experimental unknown unknown
DB04005 Uridine 5&,39;-Triphosphate experimental unknown unknown
DB04272 Citric Acid nutraceutical, vet_approved unknown unknown

Protein Secondary Structure       (annotations from PDB)      top

PDB Accession Method Resolution Chain Structure Preview
1UDW X-ray 2.6Å A/B=1-250.
1UEI X-ray 2.6Å A/B=1-250.
1UEJ X-ray 2.6Å A/B=1-250.
1UFQ X-ray 2.5Å A/B/C/D=1-250.
1UJ2 X-ray 1.8Å A/B=1-250.
1XRJ X-ray 2.0Å A/B=1-261.

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

HIV Partner Interaction Type PubMed
nucleocapsid upregulates 18051367

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
hsa00240 Pyrimidine metabolism - Homo sapiens (human)
hsa00983 Drug metabolism - other enzymes - Homo sapiens (human)
hsa01100 Metabolic pathways - Homo sapiens (human)