Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0003219
UniProt IDQ9NRR5
Primary gene name(s)UBQLN4
Synonym gene name(s)C1orf6, CIP75, UBIN
Protein nameUbiquilin-4
Protein functionPlays a role in the regulation of protein degradation via the ubiquitin-proteasome system, UPS. Mediates the proteasomal targeting of misfolded or accumulated proteins for degradation by binding, via UBA domain to their polyubiquitin chains and by interacting, via ubiquitin-like domain with the subunits of the proteasome, Ref. 6. Plays a role in the regulation of the proteasomal degradation of non-ubiquitinated GJA1, By similarity. Acts as an adapter protein that recruits UBQLN1 to the autophagy machinery. Mediates the association of UBQLN1 with autophagosomes and the autophagy-related protein LC3, MAP1LC3A/B/C and may assist in the maturation of autophagosomes to autolysosomes by mediating autophagosome-lysosome fusion, PubMed:23459205. {ECO:0000250|UniProtKB:Q99NB8, ECO:0000269|PubMed:15280365, ECO:0000269|PubMed:23459205}.
Subcellular locationNucleus {ECO:0000269|PubMed:11001934, ECO:0000269|PubMed:15280365}. Cytoplasm {ECO:0000269|PubMed:15280365}. Endoplasmic reticulum {ECO:0000250|UniProtKB:Q99NB8}. Cytoplasm, perinuclear region {ECO:0000250|UniProtKB:Q99NB8}. Cytoplasmic vesicle, autophagosome {ECO:0000269|PubMed:23459205}. Note=Colocalizes with the proteasome, both in nucleus and cytoplasm. {ECO:0000269|PubMed:15280365}.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: Q9NRR5
Gene Ontology
(Biological Process)
Complete annatation
autophagy [GO:0006914];
negative regulation of autophagosome maturation [GO:1901097];
regulation of proteasomal ubiquitin-dependent protein catabolic process [GO:0032434]
Gene Ontology
(Molecular Function)
Complete annatation
identical protein binding [GO:0042802];
polyubiquitin binding [GO:0031593]
Gene Ontology
(Cellular Component)
Complete annatation
autophagosome [GO:0005776];
cytoplasm [GO:0005737];
cytoplasmic vesicle [GO:0031410];
cytosol [GO:0005829];
endoplasmic reticulum membrane [GO:0005789];
nucleus [GO:0005634];
perinuclear region of cytoplasm [GO:0048471]
Protein-protein interaction121223
Phylogenetic treeQ9NRR5
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD31.525960170763466.93069359757326e-050.00034819804634208
AZA vs. DISU0.2821444695043320.268091276404510.843267472087723
AZA vs. IL70.1254236193301520.5151234845905750.999311006273513
AZA vs. SAHA0.135715743660140.5788758604124650.860331614464339
DISU vs. CD3-1.256374738321420.0008834069922654120.00372384703618815
DISU vs. IL7-0.1659197223553910.5122852488994040.83301939459622
DISU vs. SAHA-0.1441649923481780.6213080030308860.880275465058394
DMSO vs. AZA0.0003221167673374110.9984715633958281
DMSO vs. CD3-1.537302238341324.23599755268178e-050.00020916192438922
DMSO vs. DISU-0.2836890008434170.2475830649726520.766764650459897
DMSO vs. IL70.1324938634034730.4622508663502410.875759470793367
DMSO vs. SAHA0.1296831921087220.5828252906464140.857000603912128
HIV vs. Mock in Activation0.2400859295058710.7598707652014040.999983755607037
HIV vs. Mock in Latency-0.007006907555627370.9662737219480250.999834320637052
IL7 vs. CD3-1.394230505284090.0001617313083182030.000885852856223715
SAHA vs. CD3-1.41351898729480.0001427343510723710.000654796551190451
SAHA vs. IL70.007889120547895910.9741834797076240.990759012311464
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) FALSE
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK -0.00394929 0.984443
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
0.97 0.964 0.913 1.063 1.036
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

not found

Protein Secondary Structure       (annotations from PDB)      top

not found

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

HIV Partner Interaction Type PubMed
HIV-1 virus replication enhanced by expression of human gene 18187620

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
hsa04141 Protein processing in endoplasmic reticulum - Homo sapiens (human)