Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0003210
UniProt IDP68036
Primary gene name(s)UBE2L3
Synonym gene name(s)UBCE7, UBCH7
Protein nameUbiquitin-conjugating enzyme E2 L3
Protein functionUbiquitin-conjugating enzyme E2 that specifically acts with HECT-type and RBR family E3 ubiquitin-protein ligases. Does not function with most RING-containing E3 ubiquitin-protein ligases because it lacks intrinsic E3-independent reactivity with lysine: in contrast, it has activity with the RBR family E3 enzymes, such as PARK2 and ARIH1, that function like function like RING-HECT hybrids. Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes 'Lys-11'-linked polyubiquitination. Involved in the selective degradation of short-lived and abnormal proteins. Down-regulated during the S-phase it is involved in progression through the cell cycle. Regulates nuclear hormone receptors transcriptional activity. May play a role in myelopoiesis. {ECO:0000269|PubMed:10888878, ECO:0000269|PubMed:15367689, ECO:0000269|PubMed:17003263, ECO:0000269|PubMed:18946090, ECO:0000269|PubMed:19340006, ECO:0000269|PubMed:20061386, ECO:0000269|PubMed:21532592}.
Subcellular locationNucleus {ECO:0000269|PubMed:11278816, ECO:0000269|PubMed:17003263}. Cytoplasm {ECO:0000269|PubMed:11278816, ECO:0000269|PubMed:17003263}.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: P68036
Gene Ontology
(Biological Process)
Complete annatation
cell cycle phase transition [GO:0044770];
cell proliferation [GO:0008283];
cellular protein modification process [GO:0006464];
cellular response to glucocorticoid stimulus [GO:0071385];
cellular response to steroid hormone stimulus [GO:0071383];
positive regulation of protein targeting to mitochondrion [GO:1903955];
positive regulation of protein ubiquitination [GO:0031398];
positive regulation of ubiquitin-protein transferase activity [GO:0051443];
protein K11-linked ubiquitination [GO:0070979];
protein polyubiquitination [GO:0000209];
protein ubiquitination [GO:0016567];
protein ubiquitination involved in ubiquitin-dependent protein catabolic process [GO:0042787];
regulation of transcription, DNA-templated [GO:0006355];
transcription, DNA-templated [GO:0006351];
ubiquitin-dependent protein catabolic process [GO:0006511]
Gene Ontology
(Molecular Function)
Complete annatation
ATP binding [GO:0005524];
enzyme binding [GO:0019899];
poly(A RNA binding [GO:0044822];
transcription coactivator activity [GO:0003713];
ubiquitin conjugating enzyme activity [GO:0061631];
ubiquitin protein ligase binding [GO:0031625];
ubiquitin-protein transferase activator activity [GO:0097027];
ubiquitin-protein transferase activity [GO:0004842]
Gene Ontology
(Cellular Component)
Complete annatation
cytoplasm [GO:0005737];
extracellular exosome [GO:0070062];
nucleus [GO:0005634];
ubiquitin ligase complex [GO:0000151]
Protein-protein interaction113180
Phylogenetic treeP68036
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD31.019128268294530.002118652481341350.00695541934192483
AZA vs. DISU0.1249933340317070.6211095430992860.960057478314726
AZA vs. IL70.2273184108492420.2374786494076120.990920320206469
AZA vs. SAHA-0.1472321840594340.5463032348094220.845740470526572
DISU vs. CD3-0.9070619322003210.01323382047276770.0366568558997611
DISU vs. IL70.09293013433553110.7120517311590280.926158744367761
DISU vs. SAHA-0.2697501625151810.3546422515239780.731618684842216
DMSO vs. AZA0.02425417115805680.8850323470602221
DMSO vs. CD3-1.00739516809170.001853259845945640.00583354775996053
DMSO vs. DISU-0.1028141046741910.6733477055973610.95498935710817
DMSO vs. IL70.2104004001252430.2422908082940030.746328960979179
DMSO vs. SAHA-0.1765176721030080.4541883130091030.784389770269265
HIV vs. Mock in Activation-0.1173957284871480.8502210416989250.999983755607037
HIV vs. Mock in Latency0.002906041202955850.985980226304390.999834320637052
IL7 vs. CD3-0.7852309594178570.01520426235218110.0420348214296452
SAHA vs. CD3-1.189679642889370.0009302898477980830.00336359597080545
SAHA vs. IL7-0.3765641783849760.1225894580443250.324536967081166
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
1.8 7.38E-06 1.2 0.013313152 1.3 0.015083856
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) FALSE
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK -0.033264 0.860152
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
1.02 1.023 0.937 1.095 1.03
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
200684_s_at 1.46 No upregulated in CD8+ cells

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

not found

Protein Secondary Structure       (annotations from PDB)      top

PDB Accession Method Resolution Chain Structure Preview
1C4Z X-ray 2.6Å D=1-154.
1FBV X-ray 2.9Å C=1-154.
3SQV X-ray 3.3Å C/D=1-154.
3SY2 X-ray 3.2Å C/D=1-154.
4Q5E X-ray 1.8Å C=1-154.
4Q5H X-ray 2.0Å C=1-154.
5HPT X-ray 2.8Å C/F=2-154.

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

HIV Partner Interaction Type PubMed
Nef relocalizes 16356860
HIV-1 virus replication enhanced by expression of human gene 18854154

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
hsa04120 Ubiquitin mediated proteolysis - Homo sapiens (human)
hsa05012 Parkinson's disease - Homo sapiens (human)