Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0003209
UniProt IDP61086
Primary gene name(s)UBE2K
Synonym gene name(s)HIP2, LIG
Protein nameUbiquitin-conjugating enzyme E2 K
Protein functionAccepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro, in the presence or in the absence of BRCA1-BARD1 E3 ubiquitin-protein ligase complex, catalyzes the synthesis of 'Lys-48'-linked polyubiquitin chains. Does not transfer ubiquitin directly to but elongates monoubiquitinated substrate protein. Mediates the selective degradation of short-lived and abnormal proteins, such as the endoplasmic reticulum-associated degradation, ERAD of misfolded lumenal proteins. Ubiquitinates huntingtin. May mediate foam cell formation by the suppression of apoptosis of lipid-bearing macrophages through ubiquitination and subsequence degradation of p53/TP53. Proposed to be involved in ubiquitination and proteolytic processing of NF-kappa-B; in vitro supports ubiquitination of NFKB1. In case of infection by cytomegaloviruses may be involved in the US11-dependent degradation of MHC class I heavy chains following their export from the ER to the cytosol. In case of viral infections may be involved in the HPV E7 protein-dependent degradation of RB1. {ECO:0000269|PubMed:10634809, ECO:0000269|PubMed:10675012, ECO:0000269|PubMed:16714285, ECO:0000269|PubMed:16868077, ECO:0000269|PubMed:17873885, ECO:0000269|PubMed:19906396, ECO:0000269|PubMed:20061386, ECO:0000269|PubMed:8702625}.
Subcellular locationCytoplasm {ECO:0000250|UniProtKB:P61085}.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: P61086
Gene Ontology
(Biological Process)
Complete annatation
cellular response to interferon-beta [GO:0035458];
free ubiquitin chain polymerization [GO:0010994];
intrinsic apoptotic signaling pathway in response to endoplasmic reticulum stress [GO:0070059];
positive regulation of peptidyl-threonine phosphorylation [GO:0010800];
positive regulation of type I interferon-mediated signaling pathway [GO:0060340];
proteasome-mediated ubiquitin-dependent protein catabolic process [GO:0043161];
protein K48-linked ubiquitination [GO:0070936];
regulation of proteasomal ubiquitin-dependent protein catabolic process [GO:0032434];
ubiquitin-dependent protein catabolic process [GO:0006511]
Gene Ontology
(Molecular Function)
Complete annatation
ATP binding [GO:0005524];
ubiquitin protein ligase binding [GO:0031625];
ubiquitin-protein transferase activity [GO:0004842];
ubiquitin-ubiquitin ligase activity [GO:0034450]
Gene Ontology
(Cellular Component)
Complete annatation
cytoplasm [GO:0005737];
extracellular exosome [GO:0070062];
filopodium tip [GO:0032433];
nucleus [GO:0005634]
Protein-protein interaction109340
Phylogenetic treeP61086
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD30.5697329791060820.08309719913205190.151169702512768
AZA vs. DISU0.2453547916387830.3323807807663570.879402251291869
AZA vs. IL70.249538513004730.1943421091612650.957179543385535
AZA vs. SAHA-0.05862278528063440.8104553328232970.951967872961237
DISU vs. CD3-0.3371598426638190.3520864582558190.485843214184201
DISU vs. IL7-0.004614686396336430.985379076293890.997756262201164
DISU vs. SAHA-0.3032888244477120.3010714503575590.685415079809482
DMSO vs. AZA-0.06317580180683460.7061665965176881
DMSO vs. CD3-0.6433652661508940.04524861582771660.088574635842587
DMSO vs. DISU-0.3100985972268680.2042156267325310.7197807889769
DMSO vs. IL70.3197223870696150.07554664071739980.519792610139115
DMSO vs. SAHA-0.003193554149955940.9892135724174390.996420114283065
HIV vs. Mock in Activation-0.09084584464290730.8841228684868370.999983755607037
HIV vs. Mock in Latency0.02675332453406650.8711800364439760.999834320637052
IL7 vs. CD3-0.3114336265599020.3326897423995660.471034424872126
SAHA vs. CD3-0.6538741053111080.06747207354181330.126973544989401
SAHA vs. IL7-0.3118924874540390.2018854655256270.434515077650989
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) FALSE
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK -0.00821695 0.971999
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
1.006 1.003 0.959 0.991 0.985
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

not found

Protein Secondary Structure       (annotations from PDB)      top

PDB Accession Method Resolution Chain Structure Preview
1YLA X-ray 2.4Å A/B=1-200.
2O25 X-ray 2.6Å A/B=1-200.
3E46 X-ray 1.8Å A=1-200.
3F92 X-ray 2.2Å A=13-200.
3K9O X-ray 1.8Å A=1-200.
3K9P X-ray 2.8Å A=1-200.
5DFL X-ray 2.1Å A=1-200.

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

not found

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
hsa04120 Ubiquitin mediated proteolysis - Homo sapiens (human)