Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0003202
UniProt IDQ9UBT2
Primary gene name(s)UBA2
Synonym gene name(s)SAE2, UBLE1B
Protein nameSUMO-activating enzyme subunit 2
Protein functionThe heterodimer acts as an E1 ligase for SUMO1, SUMO2, SUMO3, and probably SUMO4. It mediates ATP-dependent activation of SUMO proteins followed by formation of a thioester bond between a SUMO protein and a conserved active site cysteine residue on UBA2/SAE2. {ECO:0000269|PubMed:11451954, ECO:0000269|PubMed:11481243, ECO:0000269|PubMed:15660128, ECO:0000269|PubMed:17643372, ECO:0000269|PubMed:19443651, ECO:0000269|PubMed:20164921}.
Subcellular locationCytoplasm. Nucleus. Note=Shuttles between the cytoplasm and the nucleus, sumoylation is required either for nuclear translocation or nuclear retention.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: Q9UBT2
Gene Ontology
(Biological Process)
Complete annatation
protein sumoylation [GO:0016925]
Gene Ontology
(Molecular Function)
Complete annatation
ATP binding [GO:0005524];
enzyme activator activity [GO:0008047];
metal ion binding [GO:0046872];
protein heterodimerization activity [GO:0046982];
SUMO activating enzyme activity [GO:0019948]
Gene Ontology
(Cellular Component)
Complete annatation
cytosol [GO:0005829];
nucleoplasm [GO:0005654];
SUMO activating enzyme complex [GO:0031510]
Protein-protein interaction115365
Phylogenetic treeQ9UBT2
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD30.9703621450585130.003285878356249380.0101677992403447
AZA vs. DISU0.02123887842212930.9330503739734330.995042856396371
AZA vs. IL70.2394337752762240.2122653385847590.977106618390875
AZA vs. SAHA-0.09397326004239790.6997346972940830.91521048390551
DISU vs. CD3-0.9616959828220750.008426255010478110.0250980329300715
DISU vs. IL70.20941643830530.4056363862535880.766431498168388
DISU vs. SAHA-0.1144954860728510.6947457921980430.909806789647164
DMSO vs. AZA-0.05797794988709340.7287291210916581
DMSO vs. CD3-1.040414001089640.001267886651887370.00420720537680248
DMSO vs. DISU-0.08127526103046290.7389522641324550.967837575700161
DMSO vs. IL70.3047989080329630.0896534456891120.556070594395694
DMSO vs. SAHA-0.04293567706871920.8553060069714590.962018897148281
HIV vs. Mock in Activation-0.1233201845659680.8429454857932590.999983755607037
HIV vs. Mock in Latency0.02215965535824460.8930652188691050.999834320637052
IL7 vs. CD3-0.7223318018137160.02507034726981310.0627925131934018
SAHA vs. CD3-1.089573877052840.002305451741242640.00732051364107175
SAHA vs. IL7-0.337366634046690.1658812044314270.38800356767173
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) FALSE
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK 0.0976355 0.545611
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
1.03 0.92 0.933 0.851 0.982
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

not found

Protein Secondary Structure       (annotations from PDB)      top

PDB Accession Method Resolution Chain Structure Preview
1Y8Q X-ray 2.2Å B/D=1-640.
1Y8R X-ray 2.7Å B/E=1-640.
2PX9 NMR - A=166-382.
3KYC X-ray 2.4Å B=1-640.
3KYD X-ray 2.6Å B=1-549.
4W5V X-ray 2.5Å B=445-561.

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

not found

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
hsa04120 Ubiquitin mediated proteolysis - Homo sapiens (human)