Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0003099
UniProt IDO14773
Primary gene name(s)TPP1
Synonym gene name(s)CLN2
Protein nameTripeptidyl-peptidase 1
Protein functionLysosomal serine protease with tripeptidyl-peptidase I activity. May act as a non-specific lysosomal peptidase which generates tripeptides from the breakdown products produced by lysosomal proteinases. Requires substrates with an unsubstituted N-terminus, By similarity. {ECO:0000250|UniProtKB:Q9EQV6}.
Subcellular locationLysosome {ECO:0000269|PubMed:19941651}. Melanosome {ECO:0000269|PubMed:12643545}. Note=Identified by mass spectrometry in melanosome fractions from stage I to stage IV. {ECO:0000269|PubMed:12643545}.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: O14773
Gene Ontology
(Biological Process)
Complete annatation
bone resorption [GO:0045453];
central nervous system development [GO:0007417];
epithelial cell differentiation [GO:0030855];
IRE1-mediated unfolded protein response [GO:0036498];
lipid metabolic process [GO:0006629];
lysosome organization [GO:0007040];
nervous system development [GO:0007399];
neuromuscular process controlling balance [GO:0050885];
peptide catabolic process [GO:0043171];
protein catabolic process [GO:0030163];
proteolysis [GO:0006508]
Gene Ontology
(Molecular Function)
Complete annatation
endopeptidase activity [GO:0004175];
metal ion binding [GO:0046872];
peptidase activity [GO:0008233];
peptide binding [GO:0042277];
serine-type endopeptidase activity [GO:0004252];
serine-type peptidase activity [GO:0008236];
tripeptidyl-peptidase activity [GO:0008240]
Gene Ontology
(Cellular Component)
Complete annatation
extracellular exosome [GO:0070062];
lysosomal lumen [GO:0043202];
lysosome [GO:0005764];
melanosome [GO:0042470];
mitochondrion [GO:0005739]
Protein-protein interaction107611
Phylogenetic treeO14773
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD3-1.713269931259493.48327993116015e-072.97956839514449e-06
AZA vs. DISU0.2239094140662680.3762066723926480.89822839898246
AZA vs. IL7-0.2657985580237870.166547520948140.934265271027657
AZA vs. SAHA0.7987401366305840.001171730168394560.0228050532115281
DISU vs. CD31.923722129193162.97606914445581e-073.24376473488986e-06
DISU vs. IL7-0.4994128750134320.04854582145249440.290638704684811
DISU vs. SAHA0.5777548838611540.04783093376355220.272536863043665
DMSO vs. AZA0.07621144709568090.6483472944939861
DMSO vs. CD31.777919852518936.50378254585249e-085.90593635040794e-07
DMSO vs. DISU-0.1495135301893010.5402841585171530.921635859189505
DMSO vs. IL7-0.3347325692113830.06248693911941370.479140695434976
DMSO vs. SAHA0.7175555975438420.002550561510937350.034684187330429
HIV vs. Mock in Activation0.2608390936182810.6753086284059850.999983755607037
HIV vs. Mock in Latency0.2502107667596660.1288163648067970.999834320637052
IL7 vs. CD31.452709426212089.1945941812499e-067.17340155765941e-05
SAHA vs. CD32.489134780923412.97378788260971e-117.02429206754363e-10
SAHA vs. IL71.062049250652921.8333041494234e-050.000499732852593279
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) TRUE
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK 0.392362 0.00302949
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
1.045 0.983 1.123 1.304 1.128
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

not found

Protein Secondary Structure       (annotations from PDB)      top

PDB Accession Method Resolution Chain Structure Preview
1R60 Model - A=196-563.
3EDY X-ray 1.8Å A=20-563.
3EE6 X-ray 2.3Å A/B=1-563.

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

not found

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
hsa04142 Lysosome - Homo sapiens (human)