Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0003096
UniProt IDP60174
Primary gene name(s)TPI1
Synonym gene name(s)TPI
Protein nameTriosephosphate isomerase
Protein functionunknown
Subcellular locationunknown
ECO codeClick here for more information.
Amino acid sequence
FASTA format: P60174
Gene Ontology
(Biological Process)
Complete annatation
canonical glycolysis [GO:0061621];
gluconeogenesis [GO:0006094];
glyceraldehyde-3-phosphate biosynthetic process [GO:0046166];
glycerol catabolic process [GO:0019563];
glycolytic process [GO:0006096];
multicellular organism development [GO:0007275];
pentose-phosphate shunt [GO:0006098]
Gene Ontology
(Molecular Function)
Complete annatation
triose-phosphate isomerase activity [GO:0004807];
ubiquitin protein ligase binding [GO:0031625]
Gene Ontology
(Cellular Component)
Complete annatation
cytosol [GO:0005829];
extracellular exosome [GO:0070062];
extracellular space [GO:0005615];
nucleus [GO:0005634]
Protein-protein interaction113020
Phylogenetic treeP60174
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD31.893344700777842.21434378611107e-082.43077855384312e-07
AZA vs. DISU-0.1276923491314560.6131952230393430.957895177103442
AZA vs. IL70.4134814651425560.03126593173548780.551414987396628
AZA vs. SAHA-0.199683173408140.4121299191969020.767442421491551
DISU vs. CD3-2.033258021138367.11056725721804e-089.17460680393703e-07
DISU vs. IL70.5316014253188140.0352295797356120.245873108571459
DISU vs. SAHA-0.06924526718847420.8119301329416320.951302109427298
DMSO vs. AZA-0.02561722240366630.8780123113796971
DMSO vs. CD3-1.92963040652885.48277989675938e-096.17324956238267e-08
DMSO vs. DISU0.1004313816252110.6801735755080640.95662303153584
DMSO vs. IL70.4463287780565770.01294516065444410.240229587781508
DMSO vs. SAHA-0.1793380850916840.4459082168129540.778080668703026
HIV vs. Mock in Activation0.07290013745001590.9066018278495190.999983755607037
HIV vs. Mock in Latency-0.01058281136441710.9679162657118210.999834320637052
IL7 vs. CD3-1.474000231848866.76038261770984e-065.45791361525122e-05
SAHA vs. CD3-2.115886728072978.8957672339518e-091.19269761757699e-07
SAHA vs. IL7-0.6148868644838250.01170295490639970.0697274080075373
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change -2.244644322
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) FALSE
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK 0.492183 0.0173282
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
1.044 0.877 0.907 0.94 0.846
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

Drugbank ID Drug Name Drug Status Pharmacological Action Drug Action
DB01695 N-Hydroxy-4-Phosphono-Butanamide experimental unknown unknown
DB03026 Phosphoglycolohydroxamic Acid experimental unknown unknown
DB03135 [2(Formyl-Hydroxy-Amino)-Ethyl]-Phosphonic Acid experimental unknown unknown
DB03314 Fluorotryptophane experimental unknown unknown
DB03379 2-Carboxyethylphosphonic Acid experimental unknown unknown
DB03900 2-Methyl-2-Propanol experimental unknown unknown
DB04326 1,3-Dihydroxyacetonephosphate experimental unknown unknown
DB04447 1,4-Dithiothreitol experimental unknown unknown
DB04510 3-Phosphoglyceric Acid experimental unknown unknown
DB02726 2-Phosphoglycolic Acid experimental unknown unknown
DB07387 3-(BUTYLSULPHONYL)-PROPANOIC ACID experimental unknown unknown
DB03132 3-(2-Benzothiazolylthio)-1-Propanesulfonic Acid experimental unknown unknown

Protein Secondary Structure       (annotations from PDB)      top

PDB Accession Method Resolution Chain Structure Preview
1HTI X-ray 2.8Å A/B=39-286.
1KLG X-ray 2.4Å C=60-74.
1KLU X-ray 1.9Å C=60-74.
1WYI X-ray 2.2Å A/B=39-286.
2IAM X-ray 2.8Å P=60-74.
2IAN X-ray 2.8Å C/H/M/R=60-74.
2JK2 X-ray 1.7Å A/B=39-286.
2VOM X-ray 1.8Å A/B/C/D=39-286.
4BR1 X-ray 1.9Å A/B=41-286.
4E41 X-ray 2.6Å C/H=60-74.
4POC X-ray 1.6Å A/B=38-286.
4POD X-ray 1.9Å A/B=38-286.
4UNK X-ray 2.0Å A/B=39-286.
4UNL X-ray 1.5Å A/B=39-286.
4ZVJ X-ray 1.7Å A/B=38-286.

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

HIV Partner Interaction Type PubMed
Vpr downregulates 23874603

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
hsa00010 Glycolysis / Gluconeogenesis - Homo sapiens (human)
hsa00051 Fructose and mannose metabolism - Homo sapiens (human)
hsa00562 Inositol phosphate metabolism - Homo sapiens (human)
hsa01100 Metabolic pathways - Homo sapiens (human)
hsa01200 Carbon metabolism - Homo sapiens (human)
hsa01230 Biosynthesis of amino acids - Homo sapiens (human)