Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0002945
UniProt IDP15923
Primary gene name(s)TCF3
Synonym gene name(s)BHLHB21, E2A, ITF1
Protein nameTranscription factor E2-alpha
Protein functionTranscriptional regulator. Involved in the initiation of neuronal differentiation. Heterodimers between TCF3 and tissue-specific basic helix-loop-helix, bHLH proteins play major roles in determining tissue-specific cell fate during embryogenesis, like muscle or early B-cell differentiation. Dimers bind DNA on E-box motifs: 5'-CANNTG-3'. Binds to the kappa-E2 site in the kappa immunoglobulin gene enhancer. Binds to IEB1 and IEB2, which are short DNA sequences in the insulin gene transcription control region. {ECO:0000250|UniProtKB:P15806}.
Subcellular locationNucleus.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: P15923
Gene Ontology
(Biological Process)
Complete annatation
B cell differentiation [GO:0030183];
B cell lineage commitment [GO:0002326];
immunoglobulin V(DJ recombination [GO:0033152];
negative regulation of transcription from RNA polymerase II promoter [GO:0000122];
positive regulation of B cell proliferation [GO:0030890];
positive regulation of cell cycle [GO:0045787];
positive regulation of muscle cell differentiation [GO:0051149];
positive regulation of neuron differentiation [GO:0045666];
positive regulation of sequence-specific DNA binding transcription factor activity [GO:0051091];
positive regulation of transcription, DNA-templated [GO:0045893];
positive regulation of transcription from RNA polymerase II promoter [GO:0045944];
regulation of G1/S transition of mitotic cell cycle [GO:2000045];
regulation of transcription, DNA-templated [GO:0006355];
transcription, DNA-templated [GO:0006351]
Gene Ontology
(Molecular Function)
Complete annatation
bHLH transcription factor binding [GO:0043425];
DNA binding [GO:0003677];
E-box binding [GO:0070888];
enhancer binding [GO:0035326];
mitogen-activated protein kinase kinase kinase binding [GO:0031435];
protein heterodimerization activity [GO:0046982];
protein homodimerization activity [GO:0042803];
repressing transcription factor binding [GO:0070491];
RNA polymerase II core promoter proximal region sequence-specific DNA binding [GO:0000978];
transcriptional repressor activity, RNA polymerase II core promoter proximal region sequence-specific binding [GO:0001078];
transcription coactivator activity [GO:0003713];
transcription factor activity, sequence-specific DNA binding [GO:0003700];
transcription factor binding [GO:0008134];
vitamin D response element binding [GO:0070644]
Gene Ontology
(Cellular Component)
Complete annatation
cytoplasm [GO:0005737];
nuclear chromatin [GO:0000790];
nucleoplasm [GO:0005654];
nucleus [GO:0005634];
protein complex [GO:0043234];
RNA polymerase II transcription factor complex [GO:0090575];
transcription factor complex [GO:0005667]
Protein-protein interaction112791
Phylogenetic treeP15923
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD30.9725855033992870.003218568887065180.0100064674305139
AZA vs. DISU0.07541336561629280.76744913326130.976021571236163
AZA vs. IL7-0.0342054311466870.8592039955145120.999311006273513
AZA vs. SAHA0.04378883022211830.8576560574386550.967133804138028
DISU vs. CD3-0.9083884216768510.01358502761517110.0374850276901134
DISU vs. IL7-0.1191893711557010.6374071799943770.892750396855013
DISU vs. SAHA-0.02932228752354310.9204445771859710.981778779774182
DMSO vs. AZA-0.0287711785738530.8641463304254041
DMSO vs. CD3-1.01275180077140.001704745531946990.00542386302825374
DMSO vs. DISU-0.1059710741155980.6657069661645560.952960443500066
DMSO vs. IL70.002079940186140830.9907929891713340.998901777988831
DMSO vs. SAHA0.06744702764397910.7750583452340280.936454000058209
HIV vs. Mock in Activation0.2520729633807290.6860153510581640.999983755607037
HIV vs. Mock in Latency-0.01104542351840910.9468447153549370.999834320637052
IL7 vs. CD3-1.00131207091540.002029322028021090.00781242530498498
SAHA vs. CD3-0.9515138513228670.007519628048025750.0202033864652787
SAHA vs. IL70.07562286466701190.7565690949963590.890699979535701
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) FALSE
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK 0.264221 0.0582167
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
0.881 0.807 0.631 0.536 0.945
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

not found

Protein Secondary Structure       (annotations from PDB)      top

PDB Accession Method Resolution Chain Structure Preview
1HLH Model - A/B=549-610.
2MH0 NMR - A=1-37.
2YPA X-ray 2.8Å B=546-616.
2YPB X-ray 2.8Å B=546-616.
3U5V X-ray 1.7Å A=563-613.

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

HIV Partner Interaction Type PubMed
Tat regulated by 7933101

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
hsa04550 Signaling pathways regulating pluripotency of stem cells - Homo sapiens (human)
hsa05166 HTLV-I infection - Homo sapiens (human)
hsa05202 Transcriptional misregulation in cancer - Homo sapiens (human)