Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0002911
UniProt IDQ86TM6
Primary gene name(s)SYVN1
Synonym gene name(s)HRD1, KIAA1810
Protein nameE3 ubiquitin-protein ligase synoviolin
Protein functionActs as an E3 ubiquitin-protein ligase which accepts ubiquitin specifically from endoplasmic reticulum-associated UBC7 E2 ligase and transfers it to substrates, promoting their degradation. Component of the endoplasmic reticulum quality control, ERQC system also called ER-associated degradation, ERAD involved in ubiquitin-dependent degradation of misfolded endoplasmic reticulum proteins. Also promotes the degradation of normal but naturally short-lived proteins such as SGK. Protects cells from ER stress-induced apoptosis. Protects neurons from apoptosis induced by polyglutamine-expanded huntingtin, HTT or unfolded GPR37 by promoting their degradation. Sequesters p53/TP53 in the cytoplasm and promotes its degradation, thereby negatively regulating its biological function in transcription, cell cycle regulation and apoptosis. {ECO:0000269|PubMed:12459480, ECO:0000269|PubMed:12646171, ECO:0000269|PubMed:12975321, ECO:0000269|PubMed:14593114, ECO:0000269|PubMed:16289116, ECO:0000269|PubMed:16847254, ECO:0000269|PubMed:17059562, ECO:0000269|PubMed:17141218, ECO:0000269|PubMed:17170702, ECO:0000269|PubMed:22607976, ECO:0000269|PubMed:26471130}.
Subcellular locationEndoplasmic reticulum membrane {ECO:0000269|PubMed:12459480, ECO:0000269|PubMed:12646171, ECO:0000269|PubMed:14593114, ECO:0000269|PubMed:16186510, ECO:0000269|PubMed:26471130};
Multi-pass membrane protein {ECO:0000269|PubMed:12459480, ECO:0000269|PubMed:12646171, ECO:0000269|PubMed:14593114, ECO:0000269|PubMed:16186510}.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: Q86TM6
Gene Ontology
(Biological Process)
Complete annatation
endoplasmic reticulum mannose trimming [GO:1904380];
endoplasmic reticulum unfolded protein response [GO:0030968];
ERAD pathway [GO:0036503];
ER-associated ubiquitin-dependent protein catabolic process [GO:0030433];
IRE1-mediated unfolded protein response [GO:0036498];
negative regulation of endoplasmic reticulum stress-induced intrinsic apoptotic signaling pathway [GO:1902236];
protein K48-linked ubiquitination [GO:0070936];
protein N-linked glycosylation via asparagine [GO:0018279];
protein stabilization [GO:0050821];
protein ubiquitination [GO:0016567];
protein ubiquitination involved in ubiquitin-dependent protein catabolic process [GO:0042787];
retrograde protein transport, ER to cytosol [GO:0030970]
Gene Ontology
(Molecular Function)
Complete annatation
ATPase binding [GO:0051117];
chaperone binding [GO:0051087];
ligase activity [GO:0016874];
ubiquitin protein ligase activity [GO:0061630];
ubiquitin protein ligase activity involved in ERAD pathway [GO:1904264];
ubiquitin-specific protease binding [GO:1990381];
unfolded protein binding [GO:0051082];
zinc ion binding [GO:0008270]
Gene Ontology
(Cellular Component)
Complete annatation
Derlin-1 retrotranslocation complex [GO:0036513];
endoplasmic reticulum [GO:0005783];
endoplasmic reticulum membrane [GO:0005789];
endoplasmic reticulum quality control compartment [GO:0044322];
Hrd1p ubiquitin ligase complex [GO:0000836];
integral component of endoplasmic reticulum membrane [GO:0030176];
membrane [GO:0016020];
nucleoplasm [GO:0005654];
smooth endoplasmic reticulum [GO:0005790]
Protein-protein interaction124085
Phylogenetic treeQ86TM6
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD3-0.2450337203140550.4567776590541890.577660083677188
AZA vs. DISU0.4807494324657210.0864613441887930.613692810075951
AZA vs. IL7-0.1909697396346490.3220268785953330.999311006273513
AZA vs. SAHA0.276448881599550.2604759928382660.632971283701257
DISU vs. CD30.7130814173911580.05601123134496990.118234603177997
DISU vs. IL7-0.6811565301019330.01891632538308410.171858485461025
DISU vs. SAHA-0.2015233382104690.5279384272152840.840097142494091
DMSO vs. AZA0.02523458107219870.8806717332731741
DMSO vs. CD30.256899112356360.4246340737590310.538976520446524
DMSO vs. DISU-0.4577593420878180.09076209055832010.552902065930659
DMSO vs. IL7-0.2085572279650530.2475786106609380.750745949294647
DMSO vs. SAHA0.2466945634166540.298946324084840.651354640781065
HIV vs. Mock in Activation0.2400096836427630.6998058354349140.999983755607037
HIV vs. Mock in Latency0.007032886200321760.9660715104285390.999834320637052
IL7 vs. CD30.05949406893640260.8539442896431490.906113580126464
SAHA vs. CD30.498544206011790.1589475110625890.251875814060275
SAHA vs. IL70.4648284156158580.05861233956540060.203953339052936
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) TRUE
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK 0.398209 0.21929
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
1.268 12.994 12.307 13.624 13.143
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

not found

Protein Secondary Structure       (annotations from PDB)      top

not found

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

not found

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
hsa04120 Ubiquitin mediated proteolysis - Homo sapiens (human)
hsa04141 Protein processing in endoplasmic reticulum - Homo sapiens (human)