Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0002890
UniProt IDQ96I99
Primary gene name(s)SUCLG2
Synonym gene name(s)unknown
Protein nameSuccinate--CoA ligase [GDP-forming] subunit beta, mitochondrial
Protein functionGTP-specific succinyl-CoA synthetase functions in the citric acid cycle, TCA, coupling the hydrolysis of succinyl-CoA to the synthesis of GTP and thus represents the only step of substrate-level phosphorylation in the TCA. The beta subunit provides nucleotide specificity of the enzyme and binds the substrate succinate, while the binding sites for coenzyme A and phosphate are found in the alpha subunit. {ECO:0000255|HAMAP-Rule:MF_03221}.
Subcellular locationMitochondrion {ECO:0000255|HAMAP-Rule:MF_03221}.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: Q96I99
Gene Ontology
(Biological Process)
Complete annatation
succinate metabolic process [GO:0006105];
succinyl-CoA metabolic process [GO:0006104];
tricarboxylic acid cycle [GO:0006099]
Gene Ontology
(Molecular Function)
Complete annatation
ATP binding [GO:0005524];
GTP binding [GO:0005525];
succinate-CoA ligase, GDP-forming activity [GO:0004776]
Gene Ontology
(Cellular Component)
Complete annatation
microtubule organizing center [GO:0005815];
mitochondrial matrix [GO:0005759];
mitochondrion [GO:0005739];
nucleus [GO:0005634];
plasma membrane [GO:0005886]
Protein-protein interaction114329
Phylogenetic treeQ96I99
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
      Yes - Two siRNA pools inhibit HIV replication and inhibition of Tat-mediated transactivation of the HIV LTR is not observed
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD3-0.9066553396821260.006934236770202550.0191135363204998
AZA vs. DISU-0.4280377129193660.09137379833000250.622680215201834
AZA vs. IL70.1124699729733450.5591715067440480.999311006273513
AZA vs. SAHA-0.4727941625858280.0544472390134290.284385890813731
DISU vs. CD30.4666705130052720.2048338095938650.324641451563235
DISU vs. IL70.5314208442428440.03543732203423130.246296740279409
DISU vs. SAHA-0.0436977931109360.881170226743780.96945856056033
DMSO vs. AZA-0.05987575576252720.7212870898832621
DMSO vs. CD30.8360928886871970.01054778017478420.0260794860221769
DMSO vs. DISU0.3664757530639650.1337692210472460.624074243817354
DMSO vs. IL70.1794591573881740.3190180753756670.798529353500786
DMSO vs. SAHA-0.4197594943967530.07681049614742820.323807269213396
HIV vs. Mock in Activation-0.314903282207620.6335770151330060.999983755607037
HIV vs. Mock in Latency0.02721497200966150.8694624479689870.999834320637052
IL7 vs. CD31.027434788746860.001719561850942130.00675676636581093
SAHA vs. CD30.4094349398020340.2493153743862910.358646686903533
SAHA vs. IL7-0.5881879662798610.01627803672053070.0878850736122064
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) FALSE
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK -0.245966 0.0799549
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
1.02 0.95 0.991 1.031 0.788
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
215772_x_at 1.33 No downregulated in CD8+ cells

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

Drugbank ID Drug Name Drug Status Pharmacological Action Drug Action
DB00139 Succinic acid approved, nutraceutical unknown unknown

Protein Secondary Structure       (annotations from PDB)      top

not found

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

HIV Partner Interaction Type PubMed
integrase interacts with 22190034
Pol interacts with 22190034
HIV-1 virus replication enhanced by expression of human gene 18976975

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
hsa00020 Citrate cycle (TCA cycle) - Homo sapiens (human)
hsa00640 Propanoate metabolism - Homo sapiens (human)
hsa01100 Metabolic pathways - Homo sapiens (human)
hsa01200 Carbon metabolism - Homo sapiens (human)