Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0002843
UniProt IDP84103
Primary gene name(s)SRSF3
Synonym gene name(s)SFRS3, SRP20
Protein nameSerine/arginine-rich splicing factor 3
Protein functionSplicing factor that specifically promotes exon-inclusion during alternative splicing, PubMed:26876937. Interaction with YTHDC1, a RNA-binding protein that recognizes and binds N6-methyladenosine, m6A-containing RNAs, promotes recruitment of SRSF3 to its mRNA-binding elements adjacent to m6A sites, leading to exon-inclusion during alternative splicing, PubMed:26876937. Also functions as export adapter involved in mRNA nuclear export such as of histone H2A, PubMed:11336712, PubMed:18364396. Binds mRNA which is thought to be transferred to the NXF1-NXT1 heterodimer for export, TAP/NXF1 pathway; enhances NXF1-NXT1 RNA-binding activity, PubMed:11336712, PubMed:18364396. RNA-binding is semi-sequence specific, PubMed:17036044. {ECO:0000269|PubMed:11336712, ECO:0000269|PubMed:17036044, ECO:0000269|PubMed:18364396, ECO:0000269|PubMed:26876937}.
Subcellular locationNucleus {ECO:0000269|PubMed:11336712}. Nucleus speckle {ECO:0000269|PubMed:26876937}. Cytoplasm {ECO:0000269|PubMed:11336712}. Note=Recruited to nuclear speckles following interaction with YTHDC1. {ECO:0000269|PubMed:26876937}.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: P84103
Gene Ontology
(Biological Process)
Complete annatation
mRNA 3'-end processing [GO:0031124];
mRNA export from nucleus [GO:0006406];
mRNA splicing, via spliceosome [GO:0000398];
regulation of mRNA splicing, via spliceosome [GO:0048024];
RNA export from nucleus [GO:0006405];
termination of RNA polymerase II transcription [GO:0006369]
Gene Ontology
(Molecular Function)
Complete annatation
nucleotide binding [GO:0000166];
poly(A RNA binding [GO:0044822];
RNA binding [GO:0003723];
sequence-specific mRNA binding [GO:1990825]
Gene Ontology
(Cellular Component)
Complete annatation
cytoplasm [GO:0005737];
nuclear speck [GO:0016607];
nucleoplasm [GO:0005654]
Protein-protein interaction112326
Phylogenetic treeP84103
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
      unknown
Brass et al., Science, 2008
      unknown
Smith et al., J. Immunol, 2010
      unknown
Interferon-stimulated
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model


DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD31.328420542099456.39796932836445e-050.00032457150359175
AZA vs. DISU0.1453414114212290.564798049389850.951925020821155
AZA vs. IL70.2831500302991670.1397126201243960.902265776835914
AZA vs. SAHA-0.4614597895658150.05849486691979770.296619463281862
DISU vs. CD3-1.196285222144640.001112495526146010.004549581541271
DISU vs. IL70.1286478879777730.6088961955094680.880294717944189
DISU vs. SAHA-0.6052548402671770.03829467798729280.239377474884484
DMSO vs. AZA0.007864756750082510.9623940259545161
DMSO vs. CD3-1.332121516346364.05383803975257e-050.000201086533210924
DMSO vs. DISU-0.1394297697883750.5668695390096350.928054274793762
DMSO vs. IL70.2824953615453320.1151144757967240.603561174685793
DMSO vs. SAHA-0.4758713906229950.04353857907207670.23268437063662
HIV vs. Mock in Activation-0.1881037294184310.7620680884246270.999983755607037
HIV vs. Mock in Latency-0.1185093943238570.4713802191766420.999834320637052
IL7 vs. CD3-1.03746315873940.001358513360876220.00552682102253369
SAHA vs. CD3-1.814227211945756.01225180751896e-075.17149741662733e-06
SAHA vs. IL7-0.7477568958183960.002202917855641080.0211070740840602
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) FALSE
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK 0.134681 0.38811
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
1.049 0.9 0.805 0.812 0.917
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

not found

Protein Secondary Structure       (annotations from PDB)      top

PDB Accession Method Resolution Chain Structure Preview
2I2Y NMR - A=1-86.
2I38 NMR - A=1-86.

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

HIV Partner Interaction Type PubMed
Tat inhibited by 25416801

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
hsa03040 Spliceosome - Homo sapiens (human)
hsa05168 Herpes simplex infection - Homo sapiens (human)
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