Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0002800
UniProt IDQ9Y5X3
Primary gene name(s)SNX5
Synonym gene name(s)unknown
Protein nameSorting nexin-5
Protein functionInvolved in several stages of intracellular trafficking. Interacts with membranes containing phosphatidylinositol 3-phosphate, PtdIns(3P or phosphatidylinositol 3,4-bisphosphate, PtdIns(3,4P2, PubMed:15561769. Acts in part as component of the retromer membrane-deforming SNX-BAR subcomplex. The SNX-BAR retromer mediates retrograde transport of cargo proteins from endosomes to the trans-Golgi network, TGN and is involved in endosome-to-plasma membrane transport for cargo protein recycling. The SNX-BAR subcomplex functions to deform the donor membrane into a tubular profile called endosome-to-TGN transport carrier, ETC, Probable. Does not have in vitro vesicle-to-membrane remodeling activity, PubMed:23085988. Involved in retrograde transport of lysosomal enzyme receptor IGF2R, PubMed:17148574, PubMed:18596235. May function as link between endosomal transport vesicles and dynactin, Probable. Plays a role in the internalization of EGFR after EGF stimulation, Probable. Involved in EGFR endosomal sorting and degradation; the function involves PIP5K1C isoform 3 and is retromer-independent, PubMed:23602387. Together with PIP5K1C isoform 3 facilitates HGS interaction with ubiquitinated EGFR, which initiates EGFR sorting to intraluminal vesicles, ILVs of the multivesicular body for subsequent lysosomal degradation, Probable. Involved in E-cadherin sorting and degradation; inhibits PIP5K1C isoform 3-mediated E-cadherin degradation, PubMed:24610942. Plays a role in macropinocytosis, PubMed:18854019, PubMed:21048941. {ECO:0000269|PubMed:18854019, ECO:0000269|PubMed:21048941, ECO:0000269|PubMed:24610942, ECO:0000303|PubMed:15561769, ECO:0000303|PubMed:19619496, ECO:0000303|PubMed:23085988}.
Subcellular locationEndosome {ECO:0000269|PubMed:11485546}. Early endosome {ECO:0000269|PubMed:17148574, ECO:0000269|PubMed:24610942}. Early endosome membrane {ECO:0000269|PubMed:15561769};
Peripheral membrane protein;
Cytoplasmic side. Cell membrane {ECO:0000269|PubMed:15561769};
Peripheral membrane protein;
Cytoplasmic side {ECO:0000269|PubMed:18854019}. Cytoplasmic vesicle membrane;
Peripheral membrane protein;
Cytoplasmic side. Cytoplasm. Cell projection, phagocytic cup. Cell projection, ruffle. Note=Recruited to the plasma membrane after EGF stimulation, which leads to increased levels of phosphatidylinositol 3,4-bisphosphate, PdtIns(3,4P2, PubMed:15561769. Detected on macropinosomes, PubMed:16968745, PubMed:21048941. Targeted to membrane ruffles in response to EGFR stimulation. {ECO:0000269|PubMed:15561769, ECO:0000269|PubMed:16968745, ECO:0000269|PubMed:21048941}.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: Q9Y5X3
Gene Ontology
(Biological Process)
Complete annatation
intracellular protein transport [GO:0006886];
pinocytosis [GO:0006907];
regulation of macroautophagy [GO:0016241];
retrograde transport, endosome to Golgi [GO:0042147];
vesicle organization [GO:0016050]
Gene Ontology
(Molecular Function)
Complete annatation
dynactin binding [GO:0034452];
phosphatidylinositol binding [GO:0035091];
protein heterodimerization activity [GO:0046982]
Gene Ontology
(Cellular Component)
Complete annatation
cytoplasmic vesicle membrane [GO:0030659];
cytosol [GO:0005829];
early endosome membrane [GO:0031901];
extrinsic component of cytoplasmic side of plasma membrane [GO:0031234];
extrinsic component of endosome membrane [GO:0031313];
macropinocytic cup [GO:0070685];
phagocytic cup [GO:0001891];
retromer, tubulation complex [GO:0030905];
retromer complex [GO:0030904];
ruffle [GO:0001726];
tubular endosome [GO:0097422]
Protein-protein interaction118022
Phylogenetic treeQ9Y5X3
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD31.063362521145150.00130869583388160.00459568117620305
AZA vs. DISU-0.07635655824556260.7627624907515510.976021571236163
AZA vs. IL70.1705766070712160.3749936681984510.999311006273513
AZA vs. SAHA-0.02245736458210840.9266911764811090.982828593138412
DISU vs. CD3-1.152140602791650.001670858409319580.00643169552147173
DISU vs. IL70.2379173809689160.3449660606000570.725928220383342
DISU vs. SAHA0.055177935037080.850121824408340.960199180883659
DMSO vs. AZA-0.07351669032170450.661013508336871
DMSO vs. CD3-1.14782040141440.0003936558843601330.0015140224401035
DMSO vs. DISU0.001217422745882120.9960208017350560.999113603185714
DMSO vs. IL70.2512180142960540.1626843721812550.669164962344509
DMSO vs. SAHA0.04438277165510930.8506810190233320.960887239233398
HIV vs. Mock in Activation-0.2615808505801460.6739559707893310.999983755607037
HIV vs. Mock in Latency0.09158972273428170.5787476126696850.999834320637052
IL7 vs. CD3-0.884860653873710.006203796699421620.0200966747583026
SAHA vs. CD3-1.110396041415060.001939101731481370.00630469538153707
SAHA vs. IL7-0.196294466282670.4207839185912910.663889728296985
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change -1.221775014
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) FALSE
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK 0.159631 0.338302
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
1.004 0.938 1.014 1.039 0.945
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

not found

Protein Secondary Structure       (annotations from PDB)      top

PDB Accession Method Resolution Chain Structure Preview
1SYS X-ray 2.4Å C=257-265.

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

not found

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
hsa04144 Endocytosis - Homo sapiens (human)