Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0002795
UniProt IDQ13573
Primary gene name(s)SNW1
Synonym gene name(s)SKIIP, SKIP
Protein nameSNW domain-containing protein 1
Protein functionInvolved in transcriptional regulation. Modulates TGF-beta-mediated transcription via association with SMAD proteins, MYOD1-mediated transcription via association with PABPN1, RB1-mediated transcriptional repression, and retinoid-X receptor, RXR- and vitamin D receptor, VDR-dependent gene transcription in a cell line-specific manner probably involving coactivators NCOA1 and GRIP1. Is involved in NOTCH1-mediated transcriptional activation. Binds to multimerized forms of Notch intracellular domain, NICD and is proposed to recruit transcriptional coactivators such as MAML1 to form an intermediate preactivation complex which associates with DNA-bound CBF-1/RBPJ to form a transcriptional activation complex by releasing SNW1 and redundant NOTCH1 NICD. Proposed to be involved in transcriptional activation by EBV EBNA2 of CBF-1/RBPJ-repressed promoters. Is recruited by HIV-1 Tat to Tat:P-TEFb:TAR RNA complexes and is involved in Tat transcription by recruitment of MYC, MEN1 and TRRAP to the HIV promoter. Functions as a splicing factor in pre-mRNA splicing. Is required in the specific splicing of CDKN1A pre-mRNA; the function probably involves the recruitment of U2AF2 to the mRNA. Is proposed to recruit PPIL1 to the spliceosome. May be involved in cyclin-D1/CCND1 mRNA stability through the SNARP complex which associates with both the 3'end of the CCND1 gene and its mRNA. {ECO:0000269|PubMed:10644367, ECO:0000269|PubMed:11278756, ECO:0000269|PubMed:11371506, ECO:0000269|PubMed:11514567, ECO:0000269|PubMed:12840015, ECO:0000269|PubMed:14985122, ECO:0000269|PubMed:15194481, ECO:0000269|PubMed:15905409, ECO:0000269|PubMed:18794151, ECO:0000269|PubMed:19818711, ECO:0000269|PubMed:21245387, ECO:0000269|PubMed:21460037, ECO:0000269|PubMed:9632709}.
Subcellular locationNucleus {ECO:0000269|PubMed:12840015}.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: Q13573
Gene Ontology
(Biological Process)
Complete annatation
cellular response to retinoic acid [GO:0071300];
intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator [GO:0042771];
mRNA splicing, via spliceosome [GO:0000398];
negative regulation of transcription, DNA-templated [GO:0045892];
negative regulation of transcription from RNA polymerase II promoter [GO:0000122];
Notch signaling pathway [GO:0007219];
positive regulation by host of viral transcription [GO:0043923];
positive regulation of histone H3-K4 methylation [GO:0051571];
positive regulation of mRNA splicing, via spliceosome [GO:0048026];
positive regulation of neurogenesis [GO:0050769];
positive regulation of transcription from RNA polymerase II promoter [GO:0045944];
positive regulation of transforming growth factor beta receptor signaling pathway [GO:0030511];
positive regulation of vitamin D receptor signaling pathway [GO:0070564];
regulation of retinoic acid receptor signaling pathway [GO:0048385];
regulation of transcription from RNA polymerase II promoter [GO:0006357];
regulation of vitamin D receptor signaling pathway [GO:0070562];
retinoic acid receptor signaling pathway [GO:0048384];
Schwann cell proliferation [GO:0014010];
transcription initiation from RNA polymerase II promoter [GO:0006367];
viral process [GO:0016032]
Gene Ontology
(Molecular Function)
Complete annatation
Notch binding [GO:0005112];
nuclear hormone receptor binding [GO:0035257];
poly(A RNA binding [GO:0044822];
retinoic acid receptor binding [GO:0042974];
SMAD binding [GO:0046332];
transcription coactivator activity [GO:0003713];
transcription corepressor activity [GO:0003714];
vitamin D receptor binding [GO:0042809]
Gene Ontology
(Cellular Component)
Complete annatation
catalytic step 2 spliceosome [GO:0071013];
chromatin [GO:0000785];
nuclear matrix [GO:0016363];
nucleoplasm [GO:0005654];
nucleus [GO:0005634];
SMAD protein complex [GO:0071141];
spliceosomal complex [GO:0005681]
Protein-protein interaction116597
Phylogenetic treeQ13573
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD30.6221429381087950.05812466799359230.113068533298841
AZA vs. DISU0.1934152271797370.4450088785986170.91814655876178
AZA vs. IL70.04302859720036080.823110071969010.999311006273513
AZA vs. SAHA-0.266248536955230.2758514134680180.649411870469608
DISU vs. CD3-0.441452893185210.2237835648287090.347120039509839
DISU vs. IL7-0.1595885987481450.5265268456790760.840724509849313
DISU vs. SAHA-0.4581797485937790.1165439159407680.438450663290893
DMSO vs. AZA-0.03175533665828810.8499552400904691
DMSO vs. CD3-0.664711420615810.03827113361230320.0768329727970878
DMSO vs. DISU-0.2267677296040030.3533129507408920.845295578681978
DMSO vs. IL70.08178043192274770.6496814991603630.925034717336681
DMSO vs. SAHA-0.2412846567890110.3067639107783710.65905044894381
HIV vs. Mock in Activation-0.02121005636517830.9727853255769370.999983755607037
HIV vs. Mock in Latency0.09294236716395050.5739589211711040.999834320637052
IL7 vs. CD3-0.5716225471031510.0757843837269850.152897606199551
SAHA vs. CD3-0.912724980103280.01030934727492070.0262984350329955
SAHA vs. IL7-0.3124779594893690.2000332735123490.432632760695727
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) FALSE
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK -0.248268 0.0688254
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
0.886 0.802 0.67 0.609 0.819
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

not found

Protein Secondary Structure       (annotations from PDB)      top

not found

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

HIV Partner Interaction Type PubMed
Tat associates with 15905409
retropepsin cleaves 22944692
HIV-1 virus replication enhanced by expression of human gene 18854154

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
hsa03040 Spliceosome - Homo sapiens (human)
hsa04330 Notch signaling pathway - Homo sapiens (human)
hsa05169 Epstein-Barr virus infection - Homo sapiens (human)
hsa05203 Viral carcinogenesis - Homo sapiens (human)