Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0002781
UniProt IDQ9HCE7
Primary gene name(s)SMURF1
Synonym gene name(s)KIAA1625
Protein nameE3 ubiquitin-protein ligase SMURF1
Protein functionE3 ubiquitin-protein ligase that acts as a negative regulator of BMP signaling pathway. Mediates ubiquitination and degradation of SMAD1 and SMAD5, 2 receptor-regulated SMADs specific for the BMP pathway. Promotes ubiquitination and subsequent proteasomal degradation of TRAF family members and RHOA. Plays a role in dendrite formation by melanocytes, PubMed:23999003. {ECO:0000269|PubMed:10458166, ECO:0000269|PubMed:19937093, ECO:0000269|PubMed:21402695, ECO:0000269|PubMed:23999003}.
Subcellular locationCytoplasm {ECO:0000269|PubMed:21572392}. Cell membrane {ECO:0000269|PubMed:21572392};
Peripheral membrane protein {ECO:0000269|PubMed:21572392};
Cytoplasmic side {ECO:0000269|PubMed:21572392}.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: Q9HCE7
Gene Ontology
(Biological Process)
Complete annatation
BMP signaling pathway [GO:0030509];
cell differentiation [GO:0030154];
ectoderm development [GO:0007398];
engulfment of target by autophagosome [GO:0061736];
negative regulation of BMP signaling pathway [GO:0030514];
negative regulation of ossification [GO:0030279];
negative regulation of transforming growth factor beta receptor signaling pathway [GO:0030512];
parkin-mediated mitophagy in response to mitochondrial depolarization [GO:0061734];
positive regulation of dendrite extension [GO:1903861];
positive regulation of protein ubiquitination involved in ubiquitin-dependent protein catabolic process [GO:2000060];
proteasome-mediated ubiquitin-dependent protein catabolic process [GO:0043161];
protein export from nucleus [GO:0006611];
protein localization to cell surface [GO:0034394];
protein polyubiquitination [GO:0000209];
protein targeting to plasma membrane [GO:0072661];
protein targeting to vacuole involved in autophagy [GO:0071211];
protein ubiquitination [GO:0016567];
protein ubiquitination involved in ubiquitin-dependent protein catabolic process [GO:0042787];
receptor catabolic process [GO:0032801];
substrate localization to autophagosome [GO:0061753];
transforming growth factor beta receptor signaling pathway [GO:0007179];
ubiquitin-dependent SMAD protein catabolic process [GO:0030579];
Wnt signaling pathway, planar cell polarity pathway [GO:0060071]
Gene Ontology
(Molecular Function)
Complete annatation
activin binding [GO:0048185];
I-SMAD binding [GO:0070411];
ligase activity [GO:0016874];
phospholipid binding [GO:0005543];
R-SMAD binding [GO:0070412];
ubiquitin protein ligase activity [GO:0061630];
ubiquitin-protein transferase activity [GO:0004842]
Gene Ontology
(Cellular Component)
Complete annatation
axon [GO:0030424];
cytoplasm [GO:0005737];
cytosol [GO:0005829];
extracellular exosome [GO:0070062];
neuronal cell body [GO:0043025];
nucleoplasm [GO:0005654];
nucleus [GO:0005634];
plasma membrane [GO:0005886]
Protein-protein interaction121411
Phylogenetic treeQ9HCE7
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD3-0.1617958248646290.622624557870960.723698414145995
AZA vs. DISU0.4523474319003020.0772527883370380.590222266291186
AZA vs. IL7-0.06318426977109630.7461518139285810.999311006273513
AZA vs. SAHA0.245959548469640.3174958295669380.688656081467151
DISU vs. CD30.600785806418380.1008563533872690.187622938536307
DISU vs. IL7-0.524316070488290.0395381141264920.261879204945899
DISU vs. SAHA-0.2045725455509940.4868083005112080.816578590594829
DMSO vs. AZA0.02959230137244990.8625213954339121
DMSO vs. CD30.17847970736580.5781966801011830.677425296452031
DMSO vs. DISU-0.425011850670850.08487529749262690.539010001354356
DMSO vs. IL7-0.08520623082852460.6407642206683530.921978745576068
DMSO vs. SAHA0.2103135184572730.3761797748789090.724339515420984
HIV vs. Mock in Activation0.1946111379063450.7554942616239140.999983755607037
HIV vs. Mock in Latency0.02902154676375470.8623358914316140.999834320637052
IL7 vs. CD30.1056424881079810.7433114350774140.828325688372162
SAHA vs. CD30.383267852008710.2798984407165270.392743901452333
SAHA vs. IL70.3056503612569870.2135085163966440.450444699253045
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) TRUE
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK 0.195839 0.679738
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
unknown unknown unknown unknown unknown
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

not found

Protein Secondary Structure       (annotations from PDB)      top

PDB Accession Method Resolution Chain Structure Preview
2LAZ NMR - A=235-267.
2LB0 NMR - A=235-267.
2LB1 NMR - A=305-340.
2LTX NMR - A=306-340.
3PYC X-ray 1.9Å A=13-140.

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

HIV Partner Interaction Type PubMed
HIV-1 virus replication enhanced by expression of human gene 18976975

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
hsa04120 Ubiquitin mediated proteolysis - Homo sapiens (human)
hsa04144 Endocytosis - Homo sapiens (human)
hsa04340 Hedgehog signaling pathway - Homo sapiens (human)
hsa04350 TGF-beta signaling pathway - Homo sapiens (human)