Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0002754
UniProt IDP48764
Primary gene name(s)SLC9A3
Synonym gene name(s)NHE3
Protein nameSodium/hydrogen exchanger 3
Protein functionInvolved in pH regulation to eliminate acids generated by active metabolism or to counter adverse environmental conditions. Major proton extruding system driven by the inward sodium ion chemical gradient, PubMed:26358773. Plays an important role in signal transduction. {ECO:0000269|PubMed:26358773}.
Subcellular locationApical cell membrane {ECO:0000269|PubMed:19088451, ECO:0000269|PubMed:26358773};
Multi-pass membrane protein {ECO:0000269|PubMed:19088451}. Note=In intestinal epithelial cells, localizes to the ileal brush border. Phosphorylation at Ser-663 by SGK1 is associated with increased abundance at the cell membrane. Angiotensin-2 enhances apical expression, By similarity. {ECO:0000250|UniProtKB:Q28362}.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: P48764
Gene Ontology
(Biological Process)
Complete annatation
ion transport [GO:0006811];
potassium ion transmembrane transport [GO:0071805];
regulation of intracellular pH [GO:0051453];
sodium ion import across plasma membrane [GO:0098719]
Gene Ontology
(Molecular Function)
Complete annatation
PDZ domain binding [GO:0030165];
sodium:proton antiporter activity [GO:0015385]
Gene Ontology
(Cellular Component)
Complete annatation
apical plasma membrane [GO:0016324];
brush border [GO:0005903];
brush border membrane [GO:0031526];
cell surface [GO:0009986];
extracellular exosome [GO:0070062];
integral component of membrane [GO:0016021];
membrane-bounded vesicle [GO:0031988];
plasma membrane [GO:0005886]
Protein-protein interaction112440
Phylogenetic treeP48764
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD3-0.1919540914544470.5797595048467230.687230456708597
AZA vs. DISU0.08505145754604810.7576449886123630.976021571236163
AZA vs. IL7-0.3323634337772480.2808886303339360.999311006273513
AZA vs. SAHA2.087420185675485.44009282066327e-155.46693061191187e-12
DISU vs. CD30.2630163822518430.4848523292622720.612115913844178
DISU vs. IL7-0.4269824239146120.1354832712986770.480907041589673
DISU vs. SAHA2.00670587858491.06067488125916e-104.09882849627098e-08
DMSO vs. AZA0.3858105074831370.22560793036661
DMSO vs. CD30.5577830877810160.09667773013506460.166337369173681
DMSO vs. DISU0.2955692260364090.3071792938546930.812254995932761
DMSO vs. IL7-0.7100905714407790.02841047580990490.343893900447525
DMSO vs. SAHA1.700122021198891.75639502941749e-116.30253083055976e-09
HIV vs. Mock in Activation-0.1598174969012520.8052445763578480.999983755607037
HIV vs. Mock in Latency-0.6108494777055780.004422068807289680.188598230131516
IL7 vs. CD3-0.1345082474638780.6931914503564190.791611086538549
SAHA vs. CD32.25827177390362.06827888149519e-093.23329489047018e-08
SAHA vs. IL72.4177208803346300
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) TRUE
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK -0.143272 0.334227
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
unknown unknown unknown unknown unknown
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

not found

Protein Secondary Structure       (annotations from PDB)      top

not found

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

HIV Partner Interaction Type PubMed
Nef imported by 24874739
HIV-1 virus replication enhanced by expression of human gene 19460752

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
hsa04964 Proximal tubule bicarbonate reclamation - Homo sapiens (human)
hsa04974 Protein digestion and absorption - Homo sapiens (human)
hsa04976 Bile secretion - Homo sapiens (human)
hsa04978 Mineral absorption - Homo sapiens (human)