Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0002752
UniProt IDQ01650
Primary gene name(s)SLC7A5
Synonym gene name(s)CD98LC, LAT1, MPE16
Protein nameLarge neutral amino acids transporter small subunit 1
Protein functionSodium-independent, high-affinity transport of large neutral amino acids such as phenylalanine, tyrosine, leucine, arginine and tryptophan, when associated with SLC3A2/4F2hc. Involved in cellular amino acid uptake. Acts as an amino acid exchanger. Involved in the transport of L-DOPA across the blood-brain barrier, and that of thyroid hormones triiodothyronine, T3 and thyroxine, T4 across the cell membrane in tissues such as placenta. Plays a role in neuronal cell proliferation, neurogenesis in brain. Involved in the uptake of methylmercury, MeHg when administered as the L-cysteine or D,L-homocysteine complexes, and hence plays a role in metal ion homeostasis and toxicity. Involved in the cellular activity of small molecular weight nitrosothiols, via the stereoselective transport of L-nitrosocysteine, L-CNSO across the transmembrane. May play an important role in high-grade gliomas. Mediates blood-to-retina L-leucine transport across the inner blood-retinal barrier which in turn may play a key role in maintaining large neutral amino acids as well as neurotransmitters in the neural retina. Acts as the major transporter of tyrosine in fibroblasts. {ECO:0000269|PubMed:10049700, ECO:0000269|PubMed:10391915, ECO:0000269|PubMed:10574970, ECO:0000269|PubMed:11311135, ECO:0000269|PubMed:11389679, ECO:0000269|PubMed:11557028, ECO:0000269|PubMed:11564694, ECO:0000269|PubMed:11742812, ECO:0000269|PubMed:12117417, ECO:0000269|PubMed:12225859, ECO:0000269|PubMed:15769744, ECO:0000269|PubMed:16496379, ECO:0000269|PubMed:18262359, ECO:0000269|PubMed:9751058}.
Subcellular locationCytoplasm, cytosol. Apical cell membrane;
Multi-pass membrane protein. Note=Located to the plasma membrane by SLC3A2/4F2hc. Localized to the apical membrane of placental syncytiophoblastic cells. Expressed in both luminal and abluminal membranes of brain capillary endothelial cells, By similarity. {ECO:0000250}.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: Q01650
Gene Ontology
(Biological Process)
Complete annatation
amino acid transport [GO:0006865];
cell differentiation [GO:0030154];
cellular amino acid metabolic process [GO:0006520];
leukocyte migration [GO:0050900];
nervous system development [GO:0007399];
neutral amino acid transport [GO:0015804];
transport [GO:0006810]
Gene Ontology
(Molecular Function)
Complete annatation
amino acid transmembrane transporter activity [GO:0015171];
antiporter activity [GO:0015297];
L-amino acid transmembrane transporter activity [GO:0015179];
neutral amino acid transmembrane transporter activity [GO:0015175];
peptide antigen binding [GO:0042605]
Gene Ontology
(Cellular Component)
Complete annatation
apical plasma membrane [GO:0016324];
cytoplasm [GO:0005737];
cytosol [GO:0005829];
extracellular exosome [GO:0070062];
integral component of plasma membrane [GO:0005887];
intracellular membrane-bounded organelle [GO:0043231];
membrane [GO:0016020];
plasma membrane [GO:0005886]
Protein-protein interaction113801
Phylogenetic treeQ01650
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
      unknown
Brass et al., Science, 2008
      unknown
Smith et al., J. Immunol, 2010
      unknown
Interferon-stimulated
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model


DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD32.987539518223997.9744433278961e-111.41901782571488e-09
AZA vs. DISU-0.3269352923029130.2448577578478370.828290719279395
AZA vs. IL70.6861809020079870.01362480106248990.388871750448342
AZA vs. SAHA-0.6999976757213020.01125879978687210.10534770204054
DISU vs. CD3-3.328514916599341.10644826634143e-123.9163431496227e-11
DISU vs. IL71.003825360027960.0004619088959935120.0163742552313114
DISU vs. SAHA-0.3688546783233710.2090834346645730.583127815370239
DMSO vs. AZA0.1373632347765910.5807560305903051
DMSO vs. CD3-2.859080053807631.35957023417177e-102.08149650180871e-09
DMSO vs. DISU0.4634111230220440.07141587905253510.499281121532083
DMSO vs. IL70.5559999966715570.02816812191351290.342447127295329
DMSO vs. SAHA-0.8418575833596450.0007676744860178890.0149478322723199
HIV vs. Mock in Activation0.2178860894250710.7942683695889250.999983755607037
HIV vs. Mock in Latency-0.1838063794340650.4395589751952370.999834320637052
IL7 vs. CD3-2.297059736880712.65635669682851e-072.95929523217266e-06
SAHA vs. CD3-3.709125095132452.44249065417534e-151.1502604424507e-13
SAHA vs. IL7-1.387940096583361.12694170673056e-065.02589328025337e-05
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) FALSE
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK 1.21272 0.000305467
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
0.985 0.994 0.767 0.687 0.756
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

Drugbank ID Drug Name Drug Status Pharmacological Action Drug Action
DB02750 S-(Methylmercury)-L-Cysteine experimental unknown unknown
DB01235 Levodopa approved unknown unknown
DB00279 Liothyronine approved, vet_approved unknown unknown
DB00451 Levothyroxine approved unknown unknown
DB00509 Dextrothyroxine approved unknown unknown
DB01042 Melphalan approved unknown unknown
DB01746 D-Leucine experimental unknown unknown
DB02556 D-Phenylalanine experimental unknown unknown

Protein Secondary Structure       (annotations from PDB)      top

not found

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

HIV Partner Interaction Type PubMed
Envelope surface glycoprotein gp160; precursor interacts with 7561057
8922468
9403839
Envelope surface glycoprotein gp160; precursor regulated by 7561057
8922468
12925876

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
hsa04150 mTOR signaling pathway - Homo sapiens (human)
hsa05230 Central carbon metabolism in cancer - Homo sapiens (human)
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