Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0002728
UniProt IDQ7Z2H8
Primary gene name(s)SLC36A1
Synonym gene name(s)PAT1
Protein nameProton-coupled amino acid transporter 1
Protein functionNeutral amino acid/proton symporter. Has a pH-dependent electrogenic transport activity for small amino acids such as glycine, alanine and proline. Besides small apolar L-amino acids, it also recognize their D-enantiomers and selected amino acid derivatives such as gamma-aminobutyric acid, By similarity. {ECO:0000250, ECO:0000269|PubMed:12809675}.
Subcellular locationCell membrane {ECO:0000250|UniProtKB:Q924A5};
Multi-pass membrane protein {ECO:0000255}. Lysosome membrane {ECO:0000269|PubMed:17897319};
Multi-pass membrane protein {ECO:0000269|PubMed:17897319}. Note=In neurons, colocalizes with the exocyst complex in the axonal processes. {ECO:0000250|UniProtKB:Q924A5}.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: Q7Z2H8
Gene Ontology
(Biological Process)
Complete annatation
amino acid transport [GO:0006865];
glycine transport [GO:0015816];
ion transport [GO:0006811];
proline transport [GO:0015824]
Gene Ontology
(Molecular Function)
Complete annatation
amino acid transmembrane transporter activity [GO:0015171];
glycine transmembrane transporter activity [GO:0015187];
hydrogen:amino acid symporter activity [GO:0005280];
hydrogen ion transmembrane transporter activity [GO:0015078];
L-alanine transmembrane transporter activity [GO:0015180];
L-proline transmembrane transporter activity [GO:0015193]
Gene Ontology
(Cellular Component)
Complete annatation
endoplasmic reticulum [GO:0005783];
integral component of membrane [GO:0016021];
lysosomal membrane [GO:0005765];
plasma membrane [GO:0005886]
Protein-protein interaction128503
Phylogenetic treeQ7Z2H8
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD30.1796922492518910.5869276503604190.6934368226254
AZA vs. DISU0.5299512165614050.1549875622735570.736295207806145
AZA vs. IL7-0.03311492493242770.8678205560601490.999311006273513
AZA vs. SAHA0.03208236068796580.8979560252066680.977360688612925
DISU vs. CD30.3354879086180870.3691501832909920.503034701042163
DISU vs. IL7-0.5734373528665950.1095882777316690.433379666003386
DISU vs. SAHA-0.4947105946747450.1640298698016870.523194532863751
DMSO vs. AZA0.07261909825944280.7027235122716161
DMSO vs. CD3-0.1168834628185120.7170307958960950.793674339901085
DMSO vs. DISU-0.4589330183555770.2046905383710090.720163528644231
DMSO vs. IL7-0.09847391897443150.5967181347514390.911970258876874
DMSO vs. SAHA-0.04634525260363020.8475761855518750.959461020163348
HIV vs. Mock in Activation0.4488231807276730.4743506665668580.999983755607037
HIV vs. Mock in Latency0.1824835226084370.2941933646851960.999834320637052
IL7 vs. CD3-0.2080384858562740.5210009096243720.648476194117933
SAHA vs. CD3-0.1714513404473080.6303626475452110.722960766574303
SAHA vs. IL70.06235576240275950.8020344910669550.913702788203909
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) TRUE
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK 0.221032 0.167596
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
unknown unknown unknown unknown unknown
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

Drugbank ID Drug Name Drug Status Pharmacological Action Drug Action
DB00160 L-Alanine approved, nutraceutical unknown unknown
DB00145 Glycine approved, nutraceutical, vet_approved unknown unknown
DB06554 Gaboxadol investigational unknown substrate
DB00659 Acamprosate approved, investigational unknown unknown
DB08847 Hydroxyproline approved, experimental unknown unknown
DB02853 D-Proline experimental unknown unknown
DB01080 Vigabatrin approved unknown unknown
DB08835 Spaglumic Acid approved unknown substrate

Protein Secondary Structure       (annotations from PDB)      top

not found

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

not found

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
hsa04974 Protein digestion and absorption - Homo sapiens (human)