Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0002660
UniProt IDO95470
Primary gene name(s)SGPL1
Synonym gene name(s)KIAA1252
Protein nameSphingosine-1-phosphate lyase 1
Protein functionCleaves phosphorylated sphingoid bases, PSBs, such as sphingosine-1-phosphate, into fatty aldehydes and phosphoethanolamine. Elevates stress-induced ceramide production and apoptosis. {ECO:0000269|PubMed:11018465, ECO:0000269|PubMed:14570870, ECO:0000269|PubMed:24809814}.
Subcellular locationEndoplasmic reticulum membrane;
Single-pass type III membrane protein.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: O95470
Gene Ontology
(Biological Process)
Complete annatation
androgen metabolic process [GO:0008209];
apoptotic signaling pathway [GO:0097190];
ceramide metabolic process [GO:0006672];
estrogen metabolic process [GO:0008210];
face morphogenesis [GO:0060325];
fatty acid metabolic process [GO:0006631];
fibroblast migration [GO:0010761];
hemopoiesis [GO:0030097];
kidney development [GO:0001822];
Leydig cell differentiation [GO:0033327];
luteinization [GO:0001553];
palate development [GO:0060021];
platelet-derived growth factor receptor signaling pathway [GO:0048008];
post-embryonic development [GO:0009791];
regulation of multicellular organism growth [GO:0040014];
skeletal system morphogenesis [GO:0048705];
spermatogenesis [GO:0007283];
sphingolipid biosynthetic process [GO:0030148];
sphingolipid catabolic process [GO:0030149];
vasculogenesis [GO:0001570]
Gene Ontology
(Molecular Function)
Complete annatation
carboxy-lyase activity [GO:0016831];
pyridoxal phosphate binding [GO:0030170];
sphinganine-1-phosphate aldolase activity [GO:0008117]
Gene Ontology
(Cellular Component)
Complete annatation
endoplasmic reticulum [GO:0005783];
endoplasmic reticulum membrane [GO:0005789];
integral component of endoplasmic reticulum membrane [GO:0030176]
Protein-protein interaction114398
Phylogenetic treeO95470
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD31.5303086228594.90847790790561e-063.25332361600498e-05
AZA vs. DISU0.07830882718580860.7580058132581670.976021571236163
AZA vs. IL7-0.03480800092465810.8576685504205740.999311006273513
AZA vs. SAHA-0.05453477480883970.8248628778454690.957464772154991
DISU vs. CD3-1.463293358823287.85929335915814e-050.000458479056098877
DISU vs. IL7-0.1226650690926190.6281243293409930.887938321906928
DISU vs. SAHA-0.1313844798683110.6535808179922820.893038170852588
DMSO vs. AZA-0.07462416492869590.6602781971303761
DMSO vs. CD3-1.612880921368718.65163573893213e-076.30560679279209e-06
DMSO vs. DISU-0.1538504641595260.530438632113550.918352129429727
DMSO vs. IL70.04674550683451520.7969111384869310.958085605924753
DMSO vs. SAHA0.01278018693414370.9572090381253110.989841712889637
HIV vs. Mock in Activation0.2283431848841520.7144788712537250.999983755607037
HIV vs. Mock in Latency0.1159436922023880.4872645461799410.999834320637052
IL7 vs. CD3-1.558931077173382.11681885720072e-061.9174766006362e-05
SAHA vs. CD3-1.609430215013711.01392732949623e-056.37192863032037e-05
SAHA vs. IL7-0.02302275770453390.9254913262025070.972727391659224
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) TRUE
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK -0.215164 0.126775
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
0.998 1.064 1.101 1.212 1.05
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

Drugbank ID Drug Name Drug Status Pharmacological Action Drug Action
DB00114 Pyridoxal Phosphate nutraceutical unknown cofactor

Protein Secondary Structure       (annotations from PDB)      top

PDB Accession Method Resolution Chain Structure Preview
4Q6R X-ray 2.4Å A/B=62-568.

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

HIV Partner Interaction Type PubMed
Vpu interacts with 22190034

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
hsa00600 Sphingolipid metabolism - Homo sapiens (human)
hsa01100 Metabolic pathways - Homo sapiens (human)
hsa04071 Sphingolipid signaling pathway - Homo sapiens (human)