Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0002636
UniProt IDP05121
Primary gene name(s)SERPINE1
Synonym gene name(s)PAI1, PLANH1
Protein namePlasminogen activator inhibitor 1
Protein functionSerine protease inhibitor. This inhibitor acts as 'bait' for tissue plasminogen activator, urokinase, protein C and matriptase-3/TMPRSS7. Its rapid interaction with PLAT may function as a major control point in the regulation of fibrinolysis. {ECO:0000269|PubMed:15853774}.
Subcellular locationSecreted.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: P05121
Gene Ontology
(Biological Process)
Complete annatation
angiogenesis [GO:0001525];
cellular response to lipopolysaccharide [GO:0071222];
chronological cell aging [GO:0001300];
circadian rhythm [GO:0007623];
defense response to Gram-negative bacterium [GO:0050829];
extracellular matrix organization [GO:0030198];
fibrinolysis [GO:0042730];
negative regulation of blood coagulation [GO:0030195];
negative regulation of cell adhesion mediated by integrin [GO:0033629];
negative regulation of cell migration [GO:0030336];
negative regulation of endopeptidase activity [GO:0010951];
negative regulation of endothelial cell apoptotic process [GO:2000352];
negative regulation of extrinsic apoptotic signaling pathway via death domain receptors [GO:1902042];
negative regulation of fibrinolysis [GO:0051918];
negative regulation of plasminogen activation [GO:0010757];
negative regulation of smooth muscle cell-matrix adhesion [GO:2000098];
negative regulation of smooth muscle cell migration [GO:0014912];
negative regulation of vascular wound healing [GO:0061044];
negative regulation of wound healing [GO:0061045];
platelet degranulation [GO:0002576];
positive regulation of angiogenesis [GO:0045766];
positive regulation of blood coagulation [GO:0030194];
positive regulation of inflammatory response [GO:0050729];
positive regulation of interleukin-8 production [GO:0032757];
positive regulation of leukotriene production involved in inflammatory response [GO:0035491];
positive regulation of monocyte chemotaxis [GO:0090026];
positive regulation of receptor-mediated endocytosis [GO:0048260];
positive regulation of transcription from RNA polymerase II promoter [GO:0045944];
regulation of receptor activity [GO:0010469]
Gene Ontology
(Molecular Function)
Complete annatation
protease binding [GO:0002020];
receptor binding [GO:0005102];
serine-type endopeptidase inhibitor activity [GO:0004867]
Gene Ontology
(Cellular Component)
Complete annatation
extracellular exosome [GO:0070062];
extracellular matrix [GO:0031012];
extracellular region [GO:0005576];
extracellular space [GO:0005615];
plasma membrane [GO:0005886];
platelet alpha granule lumen [GO:0031093]
Protein-protein interaction111091
Phylogenetic treeP05121
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD3-0.4657488620761310.3916664813958330.513187621281231
AZA vs. DISU-0.1970955857686220.7859505147168870.978868222906149
AZA vs. IL7-0.4871850542705040.3796078733285380.999311006273513
AZA vs. SAHA-0.04954092187281140.9428128881493920.988407703591609
DISU vs. CD30.2538064186535620.7105274819761620.796911552329514
DISU vs. IL7-0.2969672411804610.6604136257739570.904097455550804
DISU vs. SAHA0.1465637542251940.8503089683907530.960199180883659
DMSO vs. AZA0.130850958599990.8383471251967771
DMSO vs. CD30.5689637511400510.295831260221150.406077260613359
DMSO vs. DISU0.3214508223462860.6633572081055730.952146968478786
DMSO vs. IL7-0.6081112308974890.2836483555014090.774015806185545
DMSO vs. SAHA-0.1857299268199940.7910154943543810.941137958112803
HIV vs. Mock in Activation-0.9767464004242120.1226195586876720.999983755607037
HIV vs. Mock in Latency-0.6264264181737660.2315087415905580.999834320637052
IL7 vs. CD3-0.009038290828605280.9836655124752520.989942633051949
SAHA vs. CD30.3854569136309690.5408302336521480.646285910803098
SAHA vs. IL70.4300289464152070.4948339801146250.724890905369082
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) TRUE
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK 1.97575 0.0077978
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
unknown unknown unknown unknown unknown
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

Drugbank ID Drug Name Drug Status Pharmacological Action Drug Action
DB00055 Drotrecogin alfa approved, investigational, withdrawn unknown unknown
DB00013 Urokinase approved, investigational, withdrawn yes unknown
DB00031 Tenecteplase approved unknown unknown
DB00197 Troglitazone withdrawn unknown antagonist
DB05254 Plasmin investigational unknown unknown
DB00009 Alteplase approved unknown unknown
DB00029 Anistreplase approved unknown unknown
DB00015 Reteplase approved unknown unknown

Protein Secondary Structure       (annotations from PDB)      top

PDB Accession Method Resolution Chain Structure Preview
1A7C X-ray 1.9Å A=24-402.
1B3K X-ray 2.9Å A/B/C/D=24-402.
1C5G X-ray 2.6Å A=1-402.
1DB2 X-ray 2.7Å A/B=26-402.
1DVM X-ray 2.4Å A/B/C/D=24-402.
1DVN X-ray 2.1Å A=24-402.
1LJ5 X-ray 1.8Å A=24-402.
1OC0 X-ray 2.2Å A=24-402.
3CVM X-ray 2.0Å A/B=21-402.
3EOX X-ray 2.6Å A=24-402.
3PB1 X-ray 2.3Å I=24-402.
3Q02 X-ray 2.3Å A/B=24-402.
3Q03 X-ray 2.6Å A/B=24-402.
3R4L X-ray 2.7Å A=24-402.
3UT3 X-ray 2.4Å A/B/C/D=28-402.
4AQH X-ray 2.4Å A/B/C=24-402.
4G8O X-ray 2.7Å A/B/C/D=28-402.
4G8R X-ray 2.1Å A/B=28-402.
4IC0 X-ray 2.3Å A/B/C/D=24-402.
5BRR X-ray 3.1Å I=24-402.
9PAI X-ray 2.7Å A=24-369# B=370-402.

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

HIV Partner Interaction Type PubMed
Pr55(Gag) incorporates 25631074
Tat modulates 9143604
Vpr downregulates 23728617
Tat upregulates 7523444

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
hsa04066 HIF-1 signaling pathway - Homo sapiens (human)
hsa04115 p53 signaling pathway - Homo sapiens (human)
hsa04390 Hippo signaling pathway - Homo sapiens (human)
hsa04610 Complement and coagulation cascades - Homo sapiens (human)
hsa04933 AGE-RAGE signaling pathway in diabetic complications - Homo sapiens (human)
hsa05142 Chagas disease (American trypanosomiasis) - Homo sapiens (human)