Protein Information (annotations from UniProt)
| Database ID | HIV0002624 |
| UniProt ID | Q15019 |
| Primary gene name(s) | SEPT2 |
| Synonym gene name(s) | DIFF6, KIAA0158, NEDD5 |
| Protein name | Septin-2 |
| Protein function | Filament-forming cytoskeletal GTPase. Required for normal organization of the actin cytoskeleton. Plays a role in the biogenesis of polarized columnar-shaped epithelium by maintaining polyglutamylated microtubules, thus facilitating efficient vesicle transport, and by impeding MAP4 binding to tubulin. Required for the progression through mitosis. Forms a scaffold at the midplane of the mitotic splindle required to maintain CENPE localization at kinetochores and consequently chromosome congression. During anaphase, may be required for chromosome segregation and spindle elongation. Plays a role in ciliogenesis and collective cell movements. In cilia, required for the integrity of the diffusion barrier at the base of the primary cilium that prevents diffusion of transmembrane proteins between the cilia and plasma membranes: probably acts by regulating the assembly of the tectonic-like complex, also named B9 complex by localizing TMEM231 protein. May play a role in the internalization of 2 intracellular microbial pathogens, Listeria monocytogenes and Shigella flexneri. {ECO:0000269|PubMed:15774761, ECO:0000269|PubMed:17803907, ECO:0000269|PubMed:18209106, ECO:0000269|PubMed:19145258}. |
| Subcellular location | Cytoplasm {ECO:0000269|PubMed:15774761}. Cytoplasm, cytoskeleton {ECO:0000269|PubMed:15774761}. Cytoplasm, cytoskeleton, spindle {ECO:0000269|PubMed:15774761}. Chromosome, centromere, kinetochore {ECO:0000269|PubMed:15774761}. Cleavage furrow {ECO:0000269|PubMed:15774761}. Midbody {ECO:0000269|PubMed:15774761}. Cytoplasm, cell cortex {ECO:0000269|PubMed:15774761}. Cell projection, cilium membrane {ECO:0000250}. Note=In metaphase cells, localized within the microtubule spindle. At the metaphase plate, in close apposition to the kinetochores of the congressed chromosomes. In cells undergoing cytokinesis, localized to the midbody, the ingressing cleavage furrow, and the central spindle. During bacterial infection, displays a collar shape structure next to actin at the pole of invading bacteria. In epithelial cells, colocalizes with polyglutamylated tubulin around the trans-Golgi network, as well as juxatnuclear and proximal Golgi apparatus. Localizes at the base of the cilia near the morphological distinction between the cilia and plasma membranes. |
| ECO code | Click here for more information. |
| Amino acid sequence FASTA format: Q15019 |
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| Gene Ontology (Biological Process) Complete annatation | cell division [GO:0051301]; cilium assembly [GO:0060271]; mitotic nuclear division [GO:0007067]; neuron projection development [GO:0031175]; regulation of L-glutamate transport [GO:0002036]; regulation of protein localization [GO:0032880]; smoothened signaling pathway [GO:0007224] |
| Gene Ontology (Molecular Function) Complete annatation | unknown |
| Gene Ontology (Cellular Component) Complete annatation | unknown |
| Protein-protein interaction | 110812 |
| Phylogenetic tree | Q15019 |
| HIV replication factor status |
Zhou et al., Cell. Host. Microbe., 2008 unknown Brass et al., Science, 2008 unknown Smith et al., J. Immunol, 2010 unknown |
| Interferon-stimulated gene status |
Lu et al., J. Virol., 2011 Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown; Schoggins JW and Rice CM, Curr. Opin. Virol., 2011 Targeted viruses: unknown Viral life cycle: unknown Mechanism related to antiviral activity: unknown |
| Anti-viral restriction factor |
Liu et al., Retrovirology, 2011 unknown (Triplicates) |
Gene Expression Profile top
Up-regulated; 
Down-regulated
For brief introduction to each study, please go to the help page.
Gene expression during HIV latency
| (1). Mohammadi et al., PLoS Pathog., 2014 Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model DMSO: Dimethyl suloxyde (negative control) - 0.0033% final SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies IL7: Interleukin-7 based stimulation DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM |
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| Experimental Condition | Log2 Fold Change | P value | Adjusted P value |
| AZA vs. CD3 | 0.649004910830255 | 0.0479364298568109 | 0.0963973143164581 |
| AZA vs. DISU | -0.130100301830232 | 0.606160272942936 | 0.955305607396123 |
| AZA vs. IL7 | 0.115078569984772 | 0.548061730180093 | 0.999311006273513 |
| AZA vs. SAHA | 0.173303453842 | 0.477498038468831 | 0.808230284472455 |
| DISU vs. CD3 | -0.791876948933644 | 0.0295863178923204 | 0.0707462176251328 |
| DISU vs. IL7 | 0.236252934590122 | 0.347365536492068 | 0.72841466710536 |
| DISU vs. SAHA | 0.304334974008063 | 0.296675237448821 | 0.67978571599558 |
| DMSO vs. AZA | -0.0169815469483492 | 0.918899744192824 | 1 |
| DMSO vs. CD3 | -0.679047316880584 | 0.0341891933043345 | 0.069876569953846 |
| DMSO vs. DISU | 0.110775368006597 | 0.64901806625944 | 0.948948448618541 |
| DMSO vs. IL7 | 0.139524682361179 | 0.436146258273119 | 0.861245067843667 |
| DMSO vs. SAHA | 0.183736084035772 | 0.435558954356642 | 0.76982631653676 |
| HIV vs. Mock in Activation | -0.069331543372618 | 0.911276683428039 | 0.999983755607037 |
| HIV vs. Mock in Latency | 0.00464796275431287 | 0.977425354806729 | 0.999834320637052 |
| IL7 vs. CD3 | -0.525635047690452 | 0.101991784515671 | 0.192840211741685 |
| SAHA vs. CD3 | -0.500861014142661 | 0.158147017823145 | 0.251029656477227 |
| SAHA vs. IL7 | 0.0545137215312609 | 0.822843878343041 | 0.92400655737358 |
| (2). Iglesias-Ussel et al., J. Virol., 2013 Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model |
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| Log2 Fold Change | P Value | ||
| unknown | unknown | ||
Gene expression during HIV infection and replication
| (1). Imbeault et al., PloS Pathog., 2012 Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells |
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| Experiment type | Log2 Fold Change | P Value | Adjusted P Value | ||
| Infected vs. Mock | unknown | unknown | unknown | ||
| Infected vs. Bystander | unknown | unknown | unknown | ||
| (2). Lefebvre et al., J. Virol., 2011 Transcriptome analysis of T-cell line (Sup T1) |
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| Log2 Fold Change | unknown | ||||
| (3). Li et al., J. Immunol., 2013 Lymphatic tissue |
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| Acute Fold Change | Acute P Value | Asymt Fold Change | Asypt P Value | AIDS Fold Change | AIDS P Value |
| unknown | unknown | unknown | unknown | unknown | unknown |
| (4). Chang et al., MBio., 2011 Transcriptome analysis of T-cell line (Sup T1) Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation |
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| Up-regulated (True) | unknown | ||||
| (5). Sherrill-Mix et al., BMC Retrovirol., 2015 Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based |
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| Test Status | Log2 Fold Change | P Value | |||
| unknown | unknown | unknown | |||
| (6). Rotger et al., PLoS Pathog., 2010 Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient (Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach) |
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| Log2 Fold Change | P Value | ||||
| unknown | unknown | ||||
Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV
| (1). Greenwood et al., Elife, 2016 Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset |
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| 6 h | 24 h | 48 h | 72 h | RTi | ||
| 1.008 | 0.928 | 1.095 | 1.168 | 1.042 | ||
| (2). Navare et al., Virology, 2012 SUP-T1 cell line |
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| FC-4hpi | P-value | FC-8hpi | P-value | FC-20hpi | P-value | Category |
| unknown | unknown | unknown | unknown | unknown | unknown | unknown |
| (3). Hyrcza et al., J. Virolo., 2007 Primary human CD4+ and CD8+ T Cells |
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| Affymetrix Prob ID | Fold Change | In CD8? | Category | |||
| unknown | unknown | unknown | unknown | |||
Protein Overview top
Drug-protein Interaction (annotations from DrugBank) top
Protein Secondary Structure (annotations from PDB) top
HIV-1 Interaction (annotations from NCBI HIV-1 Interaction Database) top
Metabolic/Signalling Pathway (annotations from KEGG database) top
| Pathway Accession Number | Description |
|---|---|
| hsa05100 | Bacterial invasion of epithelial cells - Homo sapiens (human) |
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