Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0002614
UniProt IDQ96EE3
Primary gene name(s)SEH1L
Synonym gene name(s)SEC13L, SEH1
Protein nameNucleoporin SEH1
Protein functionComponent of the Nup107-160 subcomplex of the nuclear pore complex, NPC. The Nup107-160 subcomplex is required for the assembly of a functional NPC. The Nup107-160 subcomplex is also required for normal kinetochore microtubule attachment, mitotic progression and chromosome segregation. This subunit plays a role in recruitment of the Nup107-160 subcomplex to the kinetochore. {ECO:0000269|PubMed:15146057, ECO:0000269|PubMed:17363900}.; FUNCTION: As a component of the GATOR subcomplex GATOR2, functions within the amino acid-sensing branch of the TORC1 signaling pathway. Indirectly activates mTORC1 and the TORC1 signaling pathway through the inhibition of the GATOR1 subcomplex, PubMed:23723238. It is negatively regulated by the upstream amino acid sensors SESN2 and GATSL3, PubMed:25457612, PubMed:27487210. {ECO:0000269|PubMed:23723238, ECO:0000269|PubMed:25457612, ECO:0000269|PubMed:27487210}.
Subcellular locationChromosome, centromere, kinetochore. Nucleus, nuclear pore complex.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: Q96EE3
Gene Ontology
(Biological Process)
Complete annatation
attachment of mitotic spindle microtubules to kinetochore [GO:0051315];
cell division [GO:0051301];
cellular protein complex localization [GO:0034629];
cellular response to amino acid starvation [GO:0034198];
cytokine production involved in inflammatory response [GO:0002534];
defense response to Gram-positive bacterium [GO:0050830];
gene silencing by RNA [GO:0031047];
intracellular transport of virus [GO:0075733];
mitotic metaphase plate congression [GO:0007080];
mitotic nuclear envelope disassembly [GO:0007077];
mRNA export from nucleus [GO:0006406];
nuclear pore organization [GO:0006999];
positive regulation of TORC1 signaling [GO:1904263];
positive regulation of TOR signaling [GO:0032008];
protein sumoylation [GO:0016925];
protein transport [GO:0015031];
regulation of cellular response to heat [GO:1900034];
regulation of glucose transport [GO:0010827];
sister chromatid cohesion [GO:0007062];
tRNA export from nucleus [GO:0006409];
viral process [GO:0016032];
viral transcription [GO:0019083]
Gene Ontology
(Molecular Function)
Complete annatation
Gene Ontology
(Cellular Component)
Complete annatation
condensed chromosome kinetochore [GO:0000777];
cytosol [GO:0005829];
GATOR2 complex [GO:0061700];
nuclear envelope [GO:0005635];
nuclear pore outer ring [GO:0031080]
Protein-protein interaction123629
Phylogenetic treeQ96EE3
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD32.265213258309543.90059096133655e-117.36713119915595e-10
AZA vs. DISU-0.2645605505661810.3004642431514480.861346716000433
AZA vs. IL70.6613924366109310.0006794896906675560.0588617707323683
AZA vs. SAHA-0.3581962493718160.148207277751530.489833274817767
DISU vs. CD3-2.542236710849343.84551279708489e-111.03328449025415e-09
DISU vs. IL70.9169801478995480.0003409672163590120.0132294305760863
DISU vs. SAHA-0.09298903644561430.7551818481153750.930767310023133
DMSO vs. AZA-0.03382774077431750.8424969197361141
DMSO vs. CD3-2.310369101895945.55888668429816e-121.0791833492133e-10
DMSO vs. DISU0.228749038296070.3532147678772260.845295578681978
DMSO vs. IL70.7025182031128040.0001155694719082720.0130523048100933
DMSO vs. SAHA-0.3316470248674180.1657877355333010.492138460158043
HIV vs. Mock in Activation-0.1355480697611110.8273702298075950.999983755607037
HIV vs. Mock in Latency-0.1452117139095880.3886317443728280.999834320637052
IL7 vs. CD3-1.595167967396761.2234207318329e-061.17009014536278e-05
SAHA vs. CD3-2.649011811069341.95876648234616e-125.66576024740052e-11
SAHA vs. IL7-1.023818920892273.72432246888987e-050.000877876691836745
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) FALSE
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK 0.00285419 0.98866
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
0.961 0.919 0.912 0.886 0.867
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

not found

Protein Secondary Structure       (annotations from PDB)      top

PDB Accession Method Resolution Chain Structure Preview
5A9Q EM 23.0Å 7/G/P/Y=1-360.

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

HIV Partner Interaction Type PubMed
Envelope surface glycoprotein gp160; precursor interacts with 22190034
Envelope surface glycoprotein gp120 interacts with 22190034

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
hsa03013 RNA transport - Homo sapiens (human)
hsa04150 mTOR signaling pathway - Homo sapiens (human)