Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0002607
UniProt IDO15027
Primary gene name(s)SEC16A
Synonym gene name(s)KIAA0310, SEC16, SEC16L
Protein nameProtein transport protein Sec16A
Protein functionDefines endoplasmic reticulum exit sites, ERES and is required for secretory cargo traffic from the endoplasmic reticulum to the Golgi apparatus. SAR1A-GTP-dependent assembly of SEC16A on the ER membrane forms an organized scaffold defining an ERES. Required for normal transitional endoplasmic reticulum, tER organization. {ECO:0000269|PubMed:17005010, ECO:0000269|PubMed:17192411}.
Subcellular locationEndoplasmic reticulum membrane;
Peripheral membrane protein. Golgi apparatus membrane {ECO:0000305};
Peripheral membrane protein {ECO:0000305}. Note=SAR1A activity is required to maintain SEC16A localization at discrete locations on the ER membrane perhaps by preventing its dissociation.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: O15027
Gene Ontology
(Biological Process)
Complete annatation
COPII vesicle coating [GO:0048208];
endoplasmic reticulum organization [GO:0007029];
protein transport [GO:0015031];
substantia nigra development [GO:0021762]
Gene Ontology
(Molecular Function)
Complete annatation
Gene Ontology
(Cellular Component)
Complete annatation
cytoplasm [GO:0005737];
cytosol [GO:0005829];
endoplasmic reticulum membrane [GO:0005789];
Golgi apparatus [GO:0005794];
Golgi membrane [GO:0000139]
Protein-protein interaction115247
Phylogenetic treeO15027
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD3-0.02652523388537980.9354464271956280.95853209264345
AZA vs. DISU0.02224893351731930.929790562008670.995042856396371
AZA vs. IL70.2506828577730080.1915849566261060.954510558301023
AZA vs. SAHA0.1151449821678760.6374860701647810.886972957510053
DISU vs. CD30.03600590526339850.9209096344561750.948680974247977
DISU vs. IL70.2191554226278760.3836592154996510.753121417320375
DISU vs. SAHA0.09538038002396170.7439023471518880.926558039167447
DMSO vs. AZA0.114880454368030.4920231806810411
DMSO vs. CD30.1289962320373870.6872152017808280.769446080626397
DMSO vs. DISU0.09061623090078730.7099081394728250.961520102633922
DMSO vs. IL70.1432871883803760.4245691433359560.855122587802628
DMSO vs. SAHA-0.004937514889999190.9833526622577860.995773299525476
HIV vs. Mock in Activation0.333885725151260.5915505130247710.999983755607037
HIV vs. Mock in Latency0.002858724376189930.9861538481501170.999834320637052
IL7 vs. CD30.2831449373903660.3788492833367750.515704438520294
SAHA vs. CD30.1180595291136640.7379007633328770.810882724590247
SAHA vs. IL7-0.1382222768753530.5712755485462540.7776941767169
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) FALSE
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK 0.00861322 0.971999
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
0.993 0.968 1.014 1.05 1.013
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

not found

Protein Secondary Structure       (annotations from PDB)      top

not found

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

not found

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
not found