Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0002591
UniProt IDQ01826
Primary gene name(s)SATB1
Synonym gene name(s)unknown
Protein nameDNA-binding protein SATB1
Protein functionCrucial silencing factor contributing to the initiation of X inactivation mediated by Xist RNA that occurs during embryogenesis and in lymphoma, By similarity. Binds to DNA at special AT-rich sequences, the consensus SATB1-binding sequence, CSBS, at nuclear matrix- or scaffold-associated regions. Thought to recognize the sugar-phosphate structure of double-stranded DNA. Transcriptional repressor controlling nuclear and viral gene expression in a phosphorylated and acetylated status-dependent manner, by binding to matrix attachment regions, MARs of DNA and inducing a local chromatin-loop remodeling. Acts as a docking site for several chromatin remodeling enzymes, e.g. PML at the MHC-I locus and also by recruiting corepressors, HDACs or coactivators, HATs directly to promoters and enhancers. Modulates genes that are essential in the maturation of the immune T-cell CD8SP from thymocytes. Required for the switching of fetal globin species, and beta- and gamma-globin genes regulation during erythroid differentiation. Plays a role in chromatin organization and nuclear architecture during apoptosis. Interacts with the unique region, UR of cytomegalovirus, CMV. Alu-like motifs and SATB1-binding sites provide a unique chromatin context which seems preferentially targeted by the HIV-1 integration machinery. Moreover, HIV-1 Tat may overcome SATB1-mediated repression of IL2 and IL2RA, interleukin in T-cells by binding to the same domain than HDAC1. Delineates specific epigenetic modifications at target gene loci, directly up-regulating metastasis-associated genes while down-regulating tumor-suppressor genes. Reprograms chromatin organization and the transcription profiles of breast tumors to promote growth and metastasis. {ECO:0000250, ECO:0000269|PubMed:10595394, ECO:0000269|PubMed:11463840, ECO:0000269|PubMed:12374985, ECO:0000269|PubMed:12692553, ECO:0000269|PubMed:1505028, ECO:0000269|PubMed:15618465, ECO:0000269|PubMed:15713622, ECO:0000269|PubMed:16377216, ECO:0000269|PubMed:16630892, ECO:0000269|PubMed:17173041, ECO:0000269|PubMed:17376900, ECO:0000269|PubMed:18337816, ECO:0000269|PubMed:19103759, ECO:0000269|PubMed:19247486, ECO:0000269|PubMed:19332023, ECO:0000269|PubMed:19430959, ECO:0000269|PubMed:9111059, ECO:0000269|PubMed:9548713}.
Subcellular locationNucleus matrix. Nucleus, PML body. Note=Organized into a cage-like network anchoring loops of heterochromatin and tethering specialized DNA sequences. When sumoylated, localized in promyelocytic leukemia nuclear bodies, PML NBs.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: Q01826
Gene Ontology
(Biological Process)
Complete annatation
activated T cell proliferation [GO:0050798];
CD4-positive, alpha-beta T cell differentiation [GO:0043367];
CD8-positive, alpha-beta T cell differentiation [GO:0043374];
cellular component disassembly involved in execution phase of apoptosis [GO:0006921];
chromatin organization [GO:0006325];
chromatin remodeling [GO:0006338];
epidermis development [GO:0008544];
histone methylation [GO:0016571];
negative regulation of transcription from RNA polymerase II promoter [GO:0000122];
reflex [GO:0060004];
transcription, DNA-templated [GO:0006351];
viral process [GO:0016032]
Gene Ontology
(Molecular Function)
Complete annatation
chromatin binding [GO:0003682];
double-stranded DNA binding [GO:0003690];
RNA polymerase II regulatory region sequence-specific DNA binding [GO:0000977];
transcriptional repressor activity, RNA polymerase II transcription regulatory region sequence-specific binding [GO:0001227]
Gene Ontology
(Cellular Component)
Complete annatation
nuclear heterochromatin [GO:0005720];
nuclear matrix [GO:0016363];
nucleoplasm [GO:0005654];
nucleus [GO:0005634];
PML body [GO:0016605]
Protein-protein interaction112211
Phylogenetic treeQ01826
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD3-0.7845548719217680.01709500306078380.0408458919042698
AZA vs. DISU-0.03895908581603130.8773101109358320.991771899371402
AZA vs. IL7-0.1536918656234110.4227962690321480.999311006273513
AZA vs. SAHA-0.2519669478816130.3017231810464880.673705308855306
DISU vs. CD30.7323487235916880.04466699797841270.0985524484084591
DISU vs. IL7-0.1233450866266820.6234985770767250.88605960675611
DISU vs. SAHA-0.2121557046060490.4659756224342730.801588700571502
DMSO vs. AZA-0.04262116687015870.7981348606638191
DMSO vs. CD30.7266409006721480.02367393842396890.0515387113232792
DMSO vs. DISU-0.00654468184390170.978544352112990.996194243487317
DMSO vs. IL7-0.1033404564284880.5641710140770150.902124083852002
DMSO vs. SAHA-0.2155891190410270.360143005390730.708649535580342
HIV vs. Mock in Activation-0.04756278500652940.9392544053502410.999983755607037
HIV vs. Mock in Latency-0.1633781791254410.3197888288769190.999834320637052
IL7 vs. CD30.6395527048925780.0479680859125220.106046195212591
SAHA vs. CD30.5069742445269760.1541737227120290.246070536037946
SAHA vs. IL7-0.102965200821780.6715786467249940.8424913882101
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) FALSE
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK -0.205112 0.142248
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
0.928 1.738 1.267 0.946 1.629
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

not found

Protein Secondary Structure       (annotations from PDB)      top

PDB Accession Method Resolution Chain Structure Preview
1YSE NMR - A=353-490.
2L1P NMR - A=179-250.
2MW8 NMR - A=641-707.
2O49 X-ray 2.0Å A=368-452.
2O4A X-ray 1.7Å A=368-452.
3NZL X-ray 1.2Å A=179-250.
3TUO X-ray 1.7Å A/B/C/D=71-171.

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

not found

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
not found