Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0002587
UniProt IDQ15020
Primary gene name(s)SART3
Synonym gene name(s)KIAA0156, TIP110
Protein nameSquamous cell carcinoma antigen recognized by T-cells 3
Protein functionU6 snRNP-binding protein that functions as a recycling factor of the splicing machinery. Promotes the initial reassembly of U4 and U6 snRNPs following their ejection from the spliceosome during its maturation, PubMed:12032085. Also binds U6atac snRNPs and may function as a recycling factor for U4atac/U6atac spliceosomal snRNP, an initial step in the assembly of U12-type spliceosomal complex. The U12-type spliceosomal complex plays a role in the splicing of introns with non-canonical splice sites, PubMed:14749385. May also function as a substrate-targeting factor for deubiquitinases like USP4 and USP15. Recruits USP4 to ubiquitinated PRPF3 within the U4/U5/U6 tri-snRNP complex, promoting PRPF3 deubiquitination and thereby regulating the spliceosome U4/U5/U6 tri-snRNP spliceosomal complex disassembly, PubMed:20595234. May also recruit the deubiquitinase USP15 to histone H2B and mediate histone deubiquitination, thereby regulating gene expression and/or DNA repair, PubMed:24526689. May play a role in hematopoiesis probably through transcription regulation of specific genes including MYC, By similarity. {ECO:0000250|UniProtKB:Q9JLI8, ECO:0000269|PubMed:12032085, ECO:0000269|PubMed:14749385, ECO:0000269|PubMed:20595234, ECO:0000269|PubMed:24526689}.; FUNCTION: Regulates Tat transactivation activity through direct interaction. May be a cellular factor for HIV-1 gene expression and viral replication. {ECO:0000269|PubMed:11959860}.
Subcellular locationNucleus, nucleoplasm {ECO:0000269|PubMed:10463607, ECO:0000269|PubMed:11959860, ECO:0000269|PubMed:12578909}. Nucleus, Cajal body {ECO:0000269|PubMed:12578909}. Nucleus speckle {ECO:0000269|PubMed:11959860}. Cytoplasm {ECO:0000269|PubMed:10463607, ECO:0000269|PubMed:11959860}.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: Q15020
Gene Ontology
(Biological Process)
Complete annatation
cell morphogenesis [GO:0000902];
hematopoietic stem cell proliferation [GO:0071425];
homeostasis of number of cells [GO:0048872];
mRNA splicing, via spliceosome [GO:0000398];
nucleosome assembly [GO:0006334];
positive regulation of histone deubiquitination [GO:1903586];
regulation of gene expression [GO:0010468];
spliceosomal complex assembly [GO:0000245];
spliceosomal snRNP assembly [GO:0000387];
spliceosomal tri-snRNP complex assembly [GO:0000244]
Gene Ontology
(Molecular Function)
Complete annatation
histone binding [GO:0042393];
nucleotide binding [GO:0000166];
poly(A RNA binding [GO:0044822];
U4 snRNA binding [GO:0030621];
U6atac snRNA binding [GO:0030624];
U6 snRNA binding [GO:0017070];
ubiquitin-specific protease binding [GO:1990381]
Gene Ontology
(Cellular Component)
Complete annatation
Cajal body [GO:0015030];
cytoplasm [GO:0005737];
nuclear speck [GO:0016607];
nucleoplasm [GO:0005654];
nucleus [GO:0005634]
Protein-protein interaction115082
Phylogenetic treeQ15020
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD30.7124989769746560.03021394863649120.0654852795296513
AZA vs. DISU0.2228534943445450.3788906509510080.898709518741011
AZA vs. IL70.1112824337417520.563058593981860.999311006273513
AZA vs. SAHA0.06297560439450720.7965064533833280.946507223785305
DISU vs. CD3-0.5027649631539380.1668331388636390.277239856160345
DISU vs. IL7-0.1207215413805050.6319231615741980.889960941050537
DISU vs. SAHA-0.1578060484652950.589461385720050.868682806399134
DMSO vs. AZA0.05666640293035750.735608707723391
DMSO vs. CD3-0.6674516286640110.03755655006798640.0756154932552984
DMSO vs. DISU-0.1680855876674910.491050603925880.905788085317748
DMSO vs. IL70.06196327286279240.7305808003997410.945941419992686
DMSO vs. SAHA0.000129782838256860.9995620945928280.999823695393046
HIV vs. Mock in Activation0.2764838791744820.656690828456270.999983755607037
HIV vs. Mock in Latency0.006383194644608820.9691934390153130.999834320637052
IL7 vs. CD3-0.5945711428214390.06490303506237450.135365081983558
SAHA vs. CD3-0.6737073468093680.05737997732081520.110974879135612
SAHA vs. IL7-0.05143820176437580.8329206343852130.927863158903602
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) FALSE
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK -0.145817 0.33954
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
1.011 0.938 0.91 0.945 0.89
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

not found

Protein Secondary Structure       (annotations from PDB)      top

PDB Accession Method Resolution Chain Structure Preview
2DO4 NMR - A=791-877.
5CTQ X-ray 2.6Å A/B/C/D=94-611.
5CTR X-ray 3.0Å A/B=278-611.
5CTT X-ray 1.7Å B=601-649.
5JJW X-ray 3.0Å A=280-578.
5JJX X-ray 2.0Å A=81-393.
5JPZ X-ray 3.0Å A/B=96-574.

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

HIV Partner Interaction Type PubMed
Tat complexes with 24316072
Tat regulated by 11959860
Tat interacts with 24217245

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
not found