Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0002583
UniProt IDQ96LT4
Primary gene name(s)SAMD8
Synonym gene name(s)unknown
Protein nameSphingomyelin synthase-related protein 1
Protein functionSphingomyelin synthases synthesize sphingolipids through transfer of a phosphatidyl head group on to the primary hydroxyl of ceramide. SAMD8 is an endoplasmic reticulum, ER transferase that has no sphingomyelin synthase activity but can convert phosphatidylethanolamine, PE and ceramide to ceramide phosphoethanolamine, CPE albeit with low product yield. Appears to operate as a ceramide sensor to control ceramide homeostasis in the endoplasmic reticulum rather than a converter of ceramides. Seems to be critical for the integrity of the early secretory pathway. {ECO:0000269|PubMed:19506037}.
Subcellular locationEndoplasmic reticulum membrane {ECO:0000269|PubMed:19506037};
Multi-pass membrane protein {ECO:0000269|PubMed:19506037}.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: Q96LT4
Gene Ontology
(Biological Process)
Complete annatation
ceramide biosynthetic process [GO:0046513];
regulation of ceramide biosynthetic process [GO:2000303];
sphingomyelin biosynthetic process [GO:0006686]
Gene Ontology
(Molecular Function)
Complete annatation
transferase activity [GO:0016740]
Gene Ontology
(Cellular Component)
Complete annatation
integral component of endoplasmic reticulum membrane [GO:0030176];
integral component of membrane [GO:0016021]
Protein-protein interaction126778
Phylogenetic treeQ96LT4
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD30.1494939806256060.6488184574710820.744215509208839
AZA vs. DISU0.5559172302029310.04600457218106180.485039366672959
AZA vs. IL7-0.01949414605564990.9199946351739490.999311006273513
AZA vs. SAHA0.2434668945794510.3217182321589850.692748186423926
DISU vs. CD30.3930058551987870.2887583409446630.419258642978217
DISU vs. IL7-0.5836271832034940.0380757463280940.2565872026899
DISU vs. SAHA-0.3115632436899410.3206820629323890.70328861618947
DMSO vs. AZA-0.06618564432114020.6966678951249481
DMSO vs. CD3-0.2268932682088380.4791415197764910.589102015460119
DMSO vs. DISU-0.6236506130873880.02234811242071420.307233904729923
DMSO vs. IL70.05386139051315760.7668542267669740.952362365362771
DMSO vs. SAHA0.3021242361567320.2032491099932510.543886245864397
HIV vs. Mock in Activation0.03865764896356490.9506864351979350.999983755607037
HIV vs. Mock in Latency0.1401604964621130.3983408857703380.999834320637052
IL7 vs. CD3-0.1605165288161380.6185537270948430.73245819219896
SAHA vs. CD30.06813278273984520.8472295659362980.891787747235424
SAHA vs. IL70.2583059875591950.292772955728490.540286931443714
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) TRUE
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK 0.381957 0.00605069
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
unknown unknown unknown unknown unknown
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

not found

Protein Secondary Structure       (annotations from PDB)      top

not found

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

not found

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
not found