Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0002575
UniProt IDP34925
Primary gene name(s)RYK
Synonym gene name(s)JTK5A
Protein nameTyrosine-protein kinase RYK
Protein functionMay be a coreceptor along with FZD8 of Wnt proteins, such as WNT1, WNT3, WNT3A and WNT5A. Involved in neuron differentiation, axon guidance, corpus callosum establishment and neurite outgrowth. In response to WNT3 stimulation, receptor C-terminal cleavage occurs in its transmembrane region and allows the C-terminal intracellular product to translocate from the cytoplasm to the nucleus where it plays a crucial role in neuronal development. {ECO:0000269|PubMed:15454084}.
Subcellular locationMembrane {ECO:0000250};
Single-pass type I membrane protein {ECO:0000250}. Nucleus {ECO:0000250}. Cytoplasm {ECO:0000250}. Note=In cells that have undergone neuronal differentiation, the C-terminal cleaved part is translocated from the cytoplasm to the nucleus. {ECO:0000250}.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: P34925
Gene Ontology
(Biological Process)
Complete annatation
axon guidance [GO:0007411];
axonogenesis [GO:0007409];
canonical Wnt signaling pathway [GO:0060070];
cell proliferation in midbrain [GO:0033278];
chemorepulsion of dopaminergic neuron axon [GO:0036518];
commissural neuron axon guidance [GO:0071679];
corpus callosum development [GO:0022038];
midbrain dopaminergic neuron differentiation [GO:1904948];
neurogenesis [GO:0022008];
neuron differentiation [GO:0030182];
neuron projection development [GO:0031175];
non-canonical Wnt signaling pathway [GO:0035567];
planar cell polarity pathway involved in axon guidance [GO:1904938];
positive regulation of MAPK cascade [GO:0043410];
signal transduction [GO:0007165];
synapse assembly [GO:0007416];
Wnt signaling pathway [GO:0016055];
Wnt signaling pathway involved in midbrain dopaminergic neuron differentiation [GO:1904953]
Gene Ontology
(Molecular Function)
Complete annatation
ATP binding [GO:0005524];
coreceptor activity involved in Wnt signaling pathway, planar cell polarity pathway [GO:1904929];
frizzled binding [GO:0005109];
transmembrane receptor protein tyrosine kinase activity [GO:0004714];
transmembrane signaling receptor activity [GO:0004888];
Wnt-activated receptor activity [GO:0042813];
Wnt-protein binding [GO:0017147]
Gene Ontology
(Cellular Component)
Complete annatation
cytoplasm [GO:0005737];
integral component of membrane [GO:0016021];
integral component of plasma membrane [GO:0005887];
membrane [GO:0016020];
nucleus [GO:0005634];
plasma membrane [GO:0005886]
Protein-protein interaction112171
Phylogenetic treeP34925
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD3-0.3136372299742180.3421328565240750.462851307201406
AZA vs. DISU0.09484841274773380.7108964623533610.973233942294547
AZA vs. IL7-0.0716995670747690.7128716418271860.999311006273513
AZA vs. SAHA0.05348064292982290.8277536098072540.958853293954856
DISU vs. CD30.3953653576756470.2863595720801820.416538086416739
DISU vs. IL7-0.1746636336025320.4943940877968880.822739402793333
DISU vs. SAHA-0.04081646904164480.8898482278674170.972691815433044
DMSO vs. AZA-0.002626507984303350.9877058506921081
DMSO vs. CD30.2971631150763150.356205782207360.470021906714012
DMSO vs. DISU-0.09984201510945550.6861282503421070.957954410639349
DMSO vs. IL7-0.06157595465224170.7355095988324480.946333083680773
DMSO vs. SAHA0.04913980268372060.8360275782691060.955395312907465
HIV vs. Mock in Activation-0.3505534463775610.5747830093280180.999983755607037
HIV vs. Mock in Latency-0.3293291247481150.08404718660298580.999834320637052
IL7 vs. CD30.2511493780972360.4372735700635810.572031967792236
SAHA vs. CD30.3413679946720320.3368748993959060.452267682307021
SAHA vs. IL70.1203811957617620.6235523098379760.812045573952281
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
-1.4 0.055052586 -1.4 0.029903468 -1.7 0.014181479
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) TRUE
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK 0.00716894 0.978732
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
unknown unknown unknown unknown unknown
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
216976_s_at 1.43 No downregulated in CD8+ cells
214172_x_at 1.41 No downregulated in CD8+ cells

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

not found

Protein Secondary Structure       (annotations from PDB)      top

not found

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

not found

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
hsa04360 Axon guidance - Homo sapiens (human)