Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0002574
UniProt IDQ9Y230
Primary gene name(s)RUVBL2
Synonym gene name(s)INO80J, TIP48, TIP49B
Protein nameRuvB-like 2
Protein functionPossesses single-stranded DNA-stimulated ATPase and ATP-dependent DNA helicase, 5' to 3' activity; hexamerization is thought to be critical for ATP hydrolysis and adjacent subunits in the ring-like structure contribute to the ATPase activity.; FUNCTION: Component of the NuA4 histone acetyltransferase complex which is involved in transcriptional activation of select genes principally by acetylation of nucleosomal histones H4 and H2A. This modification may both alter nucleosome - DNA interactions and promote interaction of the modified histones with other proteins which positively regulate transcription. This complex may be required for the activation of transcriptional programs associated with oncogene and proto-oncogene mediated growth induction, tumor suppressor mediated growth arrest and replicative senescence, apoptosis, and DNA repair. The NuA4 complex ATPase and helicase activities seem to be, at least in part, contributed by the association of RUVBL1 and RUVBL2 with EP400. NuA4 may also play a direct role in DNA repair when recruited to sites of DNA damage. Component of a SWR1-like complex that specifically mediates the removal of histone H2A.Z/H2AFZ from the nucleosome.; FUNCTION: Proposed core component of the chromatin remodeling INO80 complex which is involved in transcriptional regulation, DNA replication and probably DNA repair.; FUNCTION: Plays an essential role in oncogenic transformation by MYC and also modulates transcriptional activation by the LEF1/TCF1-CTNNB1 complex. May also inhibit the transcriptional activity of ATF2.; FUNCTION: Involved in the endoplasmic reticulum, ER-associated degradation, ERAD pathway where it negatively regulates expression of ER stress response genes. {ECO:0000269|PubMed:25652260}.
Subcellular locationNucleus matrix. Nucleus, nucleoplasm. Cytoplasm. Membrane. Note=Mainly localized in the nucleus, associated with nuclear matrix or in the nuclear cytosol. Although it is also present in the cytoplasm and associated with the cell membranes.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: Q9Y230
Gene Ontology
(Biological Process)
Complete annatation
box C/D snoRNP assembly [GO:0000492];
cellular response to estradiol stimulus [GO:0071392];
cellular response to UV [GO:0034644];
chromatin remodeling [GO:0006338];
DNA recombination [GO:0006310];
DNA repair [GO:0006281];
establishment of protein localization to chromatin [GO:0071169];
histone H2A acetylation [GO:0043968];
histone H4 acetylation [GO:0043967];
negative regulation of estrogen receptor binding [GO:0071899];
positive regulation of histone acetylation [GO:0035066];
positive regulation of telomerase RNA localization to Cajal body [GO:1904874];
positive regulation of transcription from RNA polymerase II promoter [GO:0045944];
protein folding [GO:0006457];
regulation of growth [GO:0040008];
transcription, DNA-templated [GO:0006351];
transcriptional activation by promoter-enhancer looping [GO:0071733]
Gene Ontology
(Molecular Function)
Complete annatation
ADP binding [GO:0043531];
ATP binding [GO:0005524];
ATP-dependent 5'-3' DNA helicase activity [GO:0043141];
ATP-dependent DNA helicase activity [GO:0004003];
chromatin DNA binding [GO:0031490];
damaged DNA binding [GO:0003684];
DNA helicase activity [GO:0003678];
identical protein binding [GO:0042802];
RNA polymerase II core promoter sequence-specific DNA binding [GO:0000979];
RNA polymerase II distal enhancer sequence-specific DNA binding [GO:0000980];
unfolded protein binding [GO:0051082]
Gene Ontology
(Cellular Component)
Complete annatation
cytoplasm [GO:0005737];
extracellular exosome [GO:0070062];
Ino80 complex [GO:0031011];
intracellular [GO:0005622];
intracellular ribonucleoprotein complex [GO:0030529];
membrane [GO:0016020];
MLL1 complex [GO:0071339];
NuA4 histone acetyltransferase complex [GO:0035267];
nuclear euchromatin [GO:0005719];
nuclear matrix [GO:0016363];
nucleoplasm [GO:0005654];
nucleus [GO:0005634];
R2TP complex [GO:0097255];
Swr1 complex [GO:0000812]
Protein-protein interaction116067
Phylogenetic treeQ9Y230
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD31.682095692448676.83549061131217e-075.48294382616801e-06
AZA vs. DISU-0.1851577613428620.4665411864923710.925725718884652
AZA vs. IL70.3617792380305130.06178801226849990.717085642588902
AZA vs. SAHA-0.108264802104890.658792848774610.897305868831662
DISU vs. CD3-1.879701247673125.52858170244086e-075.68858980085963e-06
DISU vs. IL70.5374313713004950.03423328521841860.241491237560908
DISU vs. SAHA0.07925590070255850.7862245180482460.942181127498044
DMSO vs. AZA0.09700491956928820.5666698609944171
DMSO vs. CD3-1.599320478875831.37116166332163e-069.58096032372001e-06
DMSO vs. DISU0.2793480349161720.2556059025618230.773706736146731
DMSO vs. IL70.2727151944151140.1318757781772490.629769015105758
DMSO vs. SAHA-0.2099113907073370.3760791367369260.724330820416895
HIV vs. Mock in Activation0.06470396287563290.9175414740587910.999983755607037
HIV vs. Mock in Latency-0.01771484133003240.9157809597230760.999834320637052
IL7 vs. CD3-1.314268659768286.83826574056079e-050.000419337589534063
SAHA vs. CD3-1.813546912253817.14504398313665e-076.0390248359994e-06
SAHA vs. IL7-0.472127344199110.05406426705611060.194224204386037
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) FALSE
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK 0.215956 0.312957
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
1.031 0.915 0.917 0.87 0.86
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

not found

Protein Secondary Structure       (annotations from PDB)      top

PDB Accession Method Resolution Chain Structure Preview
2CQA NMR - A=132-213.
2XSZ X-ray 3.0Å D/E/F=2-133# D/E/F=238-463.
3UK6 X-ray 2.9Å A/B/C/D/E/F/G/H/I/J/K/L=1-132# A/B/C/D/E/F/G/H/I/J/K/L=239-463.

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

HIV Partner Interaction Type PubMed
Pr55(Gag) inhibited by 26211835
Envelope transmembrane glycoprotein gp41 antagonizes 26211835
matrix interacts with 26211835
HIV-1 virus replication enhanced by expression of human gene 26211835
Tat interacts with 19454010

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
not found