Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0002573
UniProt IDQ9Y265
Primary gene name(s)RUVBL1
Synonym gene name(s)INO80H, NMP238, TIP49, TIP49A
Protein nameRuvB-like 1
Protein functionPossesses single-stranded DNA-stimulated ATPase and ATP-dependent DNA helicase, 3' to 5' activity; hexamerization is thought to be critical for ATP hydrolysis and adjacent subunits in the ring-like structure contribute to the ATPase activity.; FUNCTION: Component of the NuA4 histone acetyltransferase complex which is involved in transcriptional activation of select genes principally by acetylation of nucleosomal histones H4 and H2A. This modification may both alter nucleosome - DNA interactions and promote interaction of the modified histones with other proteins which positively regulate transcription. This complex may be required for the activation of transcriptional programs associated with oncogene and proto-oncogene mediated growth induction, tumor suppressor mediated growth arrest and replicative senescence, apoptosis, and DNA repair. The NuA4 complex ATPase and helicase activities seem to be, at least in part, contributed by the association of RUVBL1 and RUVBL2 with EP400. NuA4 may also play a direct role in DNA repair when recruited to sites of DNA damage. Component of a SWR1-like complex that specifically mediates the removal of histone H2A.Z/H2AFZ from the nucleosome.; FUNCTION: Proposed core component of the chromatin remodeling INO80 complex which is involved in transcriptional regulation, DNA replication and probably DNA repair.; FUNCTION: Plays an essential role in oncogenic transformation by MYC and also modulates transcriptional activation by the LEF1/TCF1-CTNNB1 complex. Essential for cell proliferation.; FUNCTION: May be able to bind plasminogen at cell surface and enhance plasminogen activation.
Subcellular locationNucleus matrix. Nucleus, nucleoplasm. Cytoplasm. Membrane. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Note=Mainly localized in the nucleus, associated with nuclear matrix or in the nuclear cytosol, although it is also present in the cytoplasm and associated with the cell membranes. In prophase and prometaphase it is located at the centrosome and the branching microtubule spindles. After mitotic nuclear membrane disintigration it accumulates at the centrosome and sites of tubulin polymerization. As cells pass through metaphase and into telophase it is located close to the centrosome at the early phase of tubulin polymerization. In anaphase it accumulates at the zone of tubule interdigitation. In telophase it is found at polar tubule overlap, and it reappears at the site of chromosomal decondensation in the daughter cells.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: Q9Y265
Gene Ontology
(Biological Process)
Complete annatation
beta-catenin-TCF complex assembly [GO:1904837];
box C/D snoRNP assembly [GO:0000492];
cell division [GO:0051301];
CENP-A containing nucleosome assembly [GO:0034080];
chromatin remodeling [GO:0006338];
DNA recombination [GO:0006310];
DNA repair [GO:0006281];
histone H2A acetylation [GO:0043968];
histone H4 acetylation [GO:0043967];
mitotic nuclear division [GO:0007067];
positive regulation of protein targeting to mitochondrion [GO:1903955];
positive regulation of telomerase RNA localization to Cajal body [GO:1904874];
regulation of growth [GO:0040008];
regulation of mitophagy [GO:1903146];
regulation of transcription from RNA polymerase II promoter [GO:0006357];
spermatogenesis [GO:0007283];
transcription, DNA-templated [GO:0006351]
Gene Ontology
(Molecular Function)
Complete annatation
ATP binding [GO:0005524];
DNA helicase activity [GO:0003678]
Gene Ontology
(Cellular Component)
Complete annatation
cytoplasm [GO:0005737];
extracellular exosome [GO:0070062];
Golgi apparatus [GO:0005794];
Ino80 complex [GO:0031011];
intracellular membrane-bounded organelle [GO:0043231];
membrane [GO:0016020];
microtubule organizing center [GO:0005815];
MLL1 complex [GO:0071339];
NuA4 histone acetyltransferase complex [GO:0035267];
nuclear matrix [GO:0016363];
nucleoplasm [GO:0005654];
nucleus [GO:0005634];
R2TP complex [GO:0097255];
Swr1 complex [GO:0000812]
Protein-protein interaction114166
Phylogenetic treeQ9Y265
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD31.581321996895122.36867236880212e-061.69197298306092e-05
AZA vs. DISU-0.4327660554874210.0892394191604060.619674296033742
AZA vs. IL70.3809507478047230.0490392860358310.647888930452026
AZA vs. SAHA-0.1392309195364120.5701372662534550.856407697938929
DISU vs. CD3-2.02629993927477.33056449986336e-089.40433440603747e-07
DISU vs. IL70.8045982793229120.001560363389882640.0365255871156533
DISU vs. SAHA0.2949385358903810.3148850033883980.697681841526984
DMSO vs. AZA0.04215986380592730.803152481218591
DMSO vs. CD3-1.552524477714212.0501828873476e-061.37744664194979e-05
DMSO vs. DISU0.472352444838550.05482148457063960.457494728776226
DMSO vs. IL70.3464961963250440.05563869728407920.463228389293554
DMSO vs. SAHA-0.18738835983060.4286542907038890.764992252553294
HIV vs. Mock in Activation-0.1026283289060520.868943330037860.999983755607037
HIV vs. Mock in Latency-0.05286178239133730.7508713208867120.999834320637052
IL7 vs. CD3-1.192559046172570.0002475882209191930.00128023919516809
SAHA vs. CD3-1.74476824891781.57305826964382e-061.21569169864269e-05
SAHA vs. IL7-0.5232314910798560.03274958257553110.138553805148346
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) FALSE
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK 0.0947738 0.895483
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
1.021 0.952 0.943 0.891 0.902
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

not found

Protein Secondary Structure       (annotations from PDB)      top

PDB Accession Method Resolution Chain Structure Preview
2C9O X-ray 2.2Å A/B/C=1-456.
2XSZ X-ray 3.0Å A/B/C=2-126# A/B/C=234-456.

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

HIV Partner Interaction Type PubMed
Rev interacts with 22174317
Pol interacts with 22190034
Tat interacts with 19454010

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
hsa04310 Wnt signaling pathway - Homo sapiens (human)