Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0002572
UniProt IDQ13761
Primary gene name(s)RUNX3
Synonym gene name(s)AML2, CBFA3, PEBP2A3
Protein nameRunt-related transcription factor 3
Protein functionCBF binds to the core site, 5'-PYGPYGGT-3', of a number of enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers, lck, IL-3 and GM-CSF promoters. In association with ZFHX3, upregulates CDKN1A promoter activity following TGF-beta stimulation, PubMed:20599712. {ECO:0000269|PubMed:20599712}.
Subcellular locationNucleus {ECO:0000255|PROSITE-ProRule:PRU00399, ECO:0000269|PubMed:20100835, ECO:0000269|PubMed:20599712}. Cytoplasm {ECO:0000269|PubMed:20100835, ECO:0000269|PubMed:20599712}. Note=The tyrosine phosphorylated form localizes to the cytoplasm. Translocates from the cytoplasm to the nucleus following TGF-beta stimulation. {ECO:0000269|PubMed:20599712}.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: Q13761
Gene Ontology
(Biological Process)
Complete annatation
chondrocyte differentiation [GO:0002062];
hemopoiesis [GO:0030097];
negative regulation of cell cycle [GO:0045786];
negative regulation of epithelial cell proliferation [GO:0050680];
negative regulation of transcription from RNA polymerase II promoter [GO:0000122];
ossification [GO:0001503];
peripheral nervous system neuron development [GO:0048935];
positive regulation of transcription, DNA-templated [GO:0045893];
protein phosphorylation [GO:0006468];
regulation of cell differentiation [GO:0045595];
regulation of transcription, DNA-templated [GO:0006355];
regulation of transcription from RNA polymerase II promoter [GO:0006357];
response to transforming growth factor beta [GO:0071559];
transcription from RNA polymerase II promoter [GO:0006366]
Gene Ontology
(Molecular Function)
Complete annatation
ATP binding [GO:0005524];
RNA polymerase II regulatory region sequence-specific DNA binding [GO:0000977];
RNA polymerase II transcription factor activity, sequence-specific DNA binding [GO:0000981];
transcription factor activity, sequence-specific DNA binding [GO:0003700]
Gene Ontology
(Cellular Component)
Complete annatation
cytoplasm [GO:0005737];
nuclear chromatin [GO:0000790];
nucleus [GO:0005634]
Protein-protein interaction107312
Phylogenetic treeQ13761
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
      unknown
Brass et al., Science, 2008
      unknown
Smith et al., J. Immunol, 2010
      unknown
Interferon-stimulated
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model


DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD31.24668391029260.0001900221741680810.000847843164316552
AZA vs. DISU0.1784070561381460.4807554415172790.931377782001655
AZA vs. IL70.01114167495486030.9537703117794160.999311006273513
AZA vs. SAHA0.2097717733375620.3894837077703210.751637508037199
DISU vs. CD3-1.080452958502390.003601092530145290.0123651181775422
DISU vs. IL7-0.176558539512480.4830094408116070.816205281945407
DISU vs. SAHA0.03382614454902460.9075831052042720.978425480408773
DMSO vs. AZA-0.009177306705452310.9562905202951061
DMSO vs. CD3-1.269422903345520.0001045964715986610.000468832444104086
DMSO vs. DISU-0.1900233729668070.4358818120697760.882284188803474
DMSO vs. IL70.02801284826385770.8760992793572660.97288360624247
DMSO vs. SAHA0.2141997077579250.3633284863431290.711502549074493
HIV vs. Mock in Activation0.2554439754674530.6811755020081550.999983755607037
HIV vs. Mock in Latency0.01268035517458650.9386651476881850.999834320637052
IL7 vs. CD3-1.229437690242310.0001878930224544770.00101002550907858
SAHA vs. CD3-1.060317086137110.003125014226990190.00952547823639606
SAHA vs. IL70.1961591824901730.420492518942820.663717720475819
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) TRUE
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK 0.149829 0.312857
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
unknown unknown unknown unknown unknown
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

not found

Protein Secondary Structure       (annotations from PDB)      top

not found

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

HIV Partner Interaction Type PubMed
Vif inhibits 23333304
Envelope surface glycoprotein gp120 upregulates 24162774

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
hsa04658 Th1 and Th2 cell differentiation - Homo sapiens (human)
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