Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0002571
UniProt IDQ7L099
Primary gene name(s)RUFY3
Synonym gene name(s)KIAA0871
Protein nameProtein RUFY3
Protein functionPlays a role in the generation of neuronal polarity formation and axon growth, By similarity. Implicated in the formation of a single axon by developing neurons, By similarity. May inhibit the formation of additional axons by inhibition of PI3K in minor neuronal processes, By similarity. Plays a role in the formation of F-actin-enriched protrusive structures at the cell periphery, PubMed:25766321. Plays a role in cytoskeletal organization by regulating the subcellular localization of FSCN1 and DBN1 at axonal growth cones, By similarity. Promotes gastric cancer cell migration and invasion in a PAK1-dependent manner, PubMed:25766321. {ECO:0000250|UniProtKB:Q5FVJ0, ECO:0000250|UniProtKB:Q9D394, ECO:0000269|PubMed:25766321}.
Subcellular locationCytoplasm {ECO:0000269|PubMed:25766321}. Endomembrane system {ECO:0000269|PubMed:20376209}. Cell projection, invadopodium {ECO:0000269|PubMed:25766321}. Perikaryon {ECO:0000250|UniProtKB:Q9D394}. Cell projection {ECO:0000250|UniProtKB:Q9D394}. Cell projection, growth cone {ECO:0000250|UniProtKB:Q9D394}. Cell projection, filopodium {ECO:0000250|UniProtKB:Q9D394}. Cell projection, lamellipodium {ECO:0000250|UniProtKB:Q9D394}. Note=Colocalizes with PAK1, F-actin, myosins and integrins in invadopodia at the cell periphery, PubMed:25766321. Colocalized with Ras-related Rab-5 proteins in cytoplasmic vesicles, PubMed:20376209. Accumulates in axon growth cones in a F-actin-dependent manner, By similarity. Colocalized with FSCN1 and F-actin at filipodia and lamellipodia of axonal growth cones, By similarity. Colocalized with DBN1 and F-actin at transitional domain of the axonal growth cone, By similarity. {ECO:0000250|UniProtKB:Q5FVJ0, ECO:0000250|UniProtKB:Q9D394, ECO:0000269|PubMed:20376209, ECO:0000269|PubMed:25766321}.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: Q7L099
Gene Ontology
(Biological Process)
Complete annatation
actin filament organization [GO:0007015];
negative regulation of axonogenesis [GO:0050771];
positive regulation of axon extension [GO:0045773];
positive regulation of cell migration [GO:0030335];
positive regulation of intracellular protein transport [GO:0090316];
regulation of establishment of cell polarity [GO:2000114]
Gene Ontology
(Molecular Function)
Complete annatation
Gene Ontology
(Cellular Component)
Complete annatation
axon [GO:0030424];
cell junction [GO:0030054];
cytoplasm [GO:0005737];
dendrite [GO:0030425];
endomembrane system [GO:0012505];
filopodium [GO:0030175];
growth cone [GO:0030426];
invadopodium [GO:0071437];
lamellipodium [GO:0030027];
membrane [GO:0016020];
perikaryon [GO:0043204]
Protein-protein interaction116566
Phylogenetic treeQ7L099
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD3-0.05398724465689150.8701374047710560.914487866031242
AZA vs. DISU0.3473062163790840.1740942771845620.760225125805357
AZA vs. IL7-0.1180395583358620.5730172224757970.999311006273513
AZA vs. SAHA0.364909800982460.1397588065634530.47480826011663
DISU vs. CD30.3869404691985980.2893982768270910.419945092885211
DISU vs. IL7-0.4732628476616950.06342999825778210.332593816951675
DISU vs. SAHA0.01805008958449440.9508909762639910.989441867353937
DMSO vs. AZA-0.03970335163677790.8179146307334381
DMSO vs. CD30.0008543145023746630.9978816369383690.998435403445761
DMSO vs. DISU-0.389210585763540.1146251530494960.590950636489531
DMSO vs. IL7-0.07111971270801790.7014800709675760.938148693207612
DMSO vs. SAHA0.3967963482133240.09715893327285840.366988040941165
HIV vs. Mock in Activation0.05429785432287530.931010510731270.999983755607037
HIV vs. Mock in Latency-0.09483818711794410.5730300222438710.999834320637052
IL7 vs. CD3-0.05452454509174590.8660696396913510.91448460098548
SAHA vs. CD30.3923162769453870.2723798374088270.384809613738729
SAHA vs. IL70.4770864701249120.0538122843632960.193549600212914
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change 1.564381499
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) TRUE
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK 0.417057 0.00475227
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
1.046 1.025 1.245 1.179 1.187
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

not found

Protein Secondary Structure       (annotations from PDB)      top

not found

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

not found

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
not found