Protein Information (annotations from UniProt)
| Database ID | HIV0002557 |
| UniProt ID | Q9HB90 |
| Primary gene name(s) | RRAGC |
| Synonym gene name(s) | unknown |
| Protein name | Ras-related GTP-binding protein C |
| Protein function | Guanine nucleotide-binding protein forming heterodimeric Rag complexes required for the amino acid-induced relocalization of mTORC1 to the lysosomes and its subsequent activation by the GTPase RHEB. This is a crucial step in the activation of the TOR signaling cascade by amino acids. {ECO:0000269|PubMed:20381137, ECO:0000269|PubMed:27234373}. |
| Subcellular location | Cytoplasm {ECO:0000269|PubMed:11073942}. Nucleus {ECO:0000269|PubMed:11073942}. Lysosome {ECO:0000269|PubMed:20381137}. Note=Predominantly cytoplasmic. May shuttle between the cytoplasm and nucleus, depending on the bound nucleotide state of associated RRAGA, PubMed:11073942. {ECO:0000269|PubMed:11073942}. |
| ECO code | Click here for more information. |
| Amino acid sequence FASTA format: Q9HB90 |
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| Gene Ontology (Biological Process) Complete annatation | apoptotic process [GO:0006915]; cell cycle arrest [GO:0007050]; cell growth [GO:0016049]; cellular protein localization [GO:0034613]; cellular response to amino acid starvation [GO:0034198]; cellular response to amino acid stimulus [GO:0071230]; macroautophagy [GO:0016236]; positive regulation of TOR signaling [GO:0032008]; regulation of autophagy [GO:0010506]; regulation of TORC1 signaling [GO:1903432]; regulation of TOR signaling [GO:0032006]; response to amino acid [GO:0043200]; RNA splicing [GO:0008380]; small GTPase mediated signal transduction [GO:0007264]; transcription, DNA-templated [GO:0006351] |
| Gene Ontology (Molecular Function) Complete annatation | GDP binding [GO:0019003]; GTPase activity [GO:0003924]; GTP binding [GO:0005525]; magnesium ion binding [GO:0000287]; protein heterodimerization activity [GO:0046982] |
| Gene Ontology (Cellular Component) Complete annatation | cytoplasm [GO:0005737]; cytosol [GO:0005829]; EGO complex [GO:0034448]; Gtr1-Gtr2 GTPase complex [GO:1990131]; intracellular membrane-bounded organelle [GO:0043231]; lysosome [GO:0005764]; nucleus [GO:0005634] |
| Protein-protein interaction | 122074 |
| Phylogenetic tree | Q9HB90 |
| HIV replication factor status |
Zhou et al., Cell. Host. Microbe., 2008 unknown Brass et al., Science, 2008 unknown Smith et al., J. Immunol, 2010 unknown |
| Interferon-stimulated gene status |
Lu et al., J. Virol., 2011 Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown; Schoggins JW and Rice CM, Curr. Opin. Virol., 2011 Targeted viruses: unknown Viral life cycle: unknown Mechanism related to antiviral activity: unknown |
| Anti-viral restriction factor |
Liu et al., Retrovirology, 2011 unknown (Triplicates) |
Gene Expression Profile top
Up-regulated; 
Down-regulated
For brief introduction to each study, please go to the help page.
Gene expression during HIV latency
| (1). Mohammadi et al., PLoS Pathog., 2014 Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model DMSO: Dimethyl suloxyde (negative control) - 0.0033% final SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies IL7: Interleukin-7 based stimulation DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM |
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| Experimental Condition | Log2 Fold Change | P value | Adjusted P value |
| AZA vs. CD3 | -1.05678172147025 | 0.00161344054359569 | 0.00551298478192569 |
| AZA vs. DISU | 0.718223570788299 | 0.00493178528501392 | 0.181764624416381 |
| AZA vs. IL7 | -0.197638131049411 | 0.308727850062207 | 0.999311006273513 |
| AZA vs. SAHA | 0.170896725671663 | 0.486094978737135 | 0.811989772770786 |
| DISU vs. CD3 | 1.76155347649129 | 2.49489395165448e-06 | 2.16511407180424e-05 |
| DISU vs. IL7 | -0.924389729125301 | 0.000291286685046188 | 0.0117527554542074 |
| DISU vs. SAHA | -0.545956449061377 | 0.0637394523616822 | 0.319141955662097 |
| DMSO vs. AZA | -0.0482000452884399 | 0.776243592150817 | 1 |
| DMSO vs. CD3 | 0.996076406419885 | 0.00230719596710904 | 0.00705887636971928 |
| DMSO vs. DISU | -0.768594600017303 | 0.00182737764832586 | 0.0881592834899272 |
| DMSO vs. IL7 | -0.142121504046244 | 0.434070483373857 | 0.860024653866327 |
| DMSO vs. SAHA | 0.212434368406351 | 0.370304346040518 | 0.717909100209254 |
| HIV vs. Mock in Activation | -0.30237791746655 | 0.628664860771812 | 0.999983755607037 |
| HIV vs. Mock in Latency | 0.0637684602068575 | 0.702819075862992 | 0.999834320637052 |
| IL7 vs. CD3 | 0.868118425758717 | 0.00799893432111631 | 0.0247962310939148 |
| SAHA vs. CD3 | 1.20229084761065 | 0.000979610905003092 | 0.00351996574592194 |
| SAHA vs. IL7 | 0.364610033064824 | 0.137206906041788 | 0.347781554006038 |
| (2). Iglesias-Ussel et al., J. Virol., 2013 Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model |
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| Log2 Fold Change | P Value | ||
| unknown | unknown | ||
Gene expression during HIV infection and replication
| (1). Imbeault et al., PloS Pathog., 2012 Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells |
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| Experiment type | Log2 Fold Change | P Value | Adjusted P Value | ||
| Infected vs. Mock | unknown | unknown | unknown | ||
| Infected vs. Bystander | unknown | unknown | unknown | ||
| (2). Lefebvre et al., J. Virol., 2011 Transcriptome analysis of T-cell line (Sup T1) |
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| Log2 Fold Change | unknown | ||||
| (3). Li et al., J. Immunol., 2013 Lymphatic tissue |
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| Acute Fold Change | Acute P Value | Asymt Fold Change | Asypt P Value | AIDS Fold Change | AIDS P Value |
| unknown | unknown | unknown | unknown | unknown | unknown |
| (4). Chang et al., MBio., 2011 Transcriptome analysis of T-cell line (Sup T1) Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation |
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| Up-regulated (True) | TRUE | ||||
| (5). Sherrill-Mix et al., BMC Retrovirol., 2015 Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based |
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| Test Status | Log2 Fold Change | P Value | |||
| OK | -0.145027 | 0.327481 | |||
| (6). Rotger et al., PLoS Pathog., 2010 Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient (Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach) |
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| Log2 Fold Change | P Value | ||||
| unknown | unknown | ||||
Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV
| (1). Greenwood et al., Elife, 2016 Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset |
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| 6 h | 24 h | 48 h | 72 h | RTi | ||
| 1.048 | 1.016 | 1.077 | 1.141 | 1.228 | ||
| (2). Navare et al., Virology, 2012 SUP-T1 cell line |
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| FC-4hpi | P-value | FC-8hpi | P-value | FC-20hpi | P-value | Category |
| unknown | unknown | unknown | unknown | unknown | unknown | unknown |
| (3). Hyrcza et al., J. Virolo., 2007 Primary human CD4+ and CD8+ T Cells |
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| Affymetrix Prob ID | Fold Change | In CD8? | Category | |||
| unknown | unknown | unknown | unknown | |||
Protein Overview top
Drug-protein Interaction (annotations from DrugBank) top
Protein Secondary Structure (annotations from PDB) top
| PDB Accession | Method | Resolution | Chain | Structure Preview |
|---|---|---|---|---|
| 3LLU | X-ray | 1.4Å | A=60-237. |  |
HIV-1 Interaction (annotations from NCBI HIV-1 Interaction Database) top
Metabolic/Signalling Pathway (annotations from KEGG database) top
| Pathway Accession Number | Description |
|---|---|
| hsa04150 | mTOR signaling pathway - Homo sapiens (human) |
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