Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0002516
UniProt IDQ9NWF9
Primary gene name(s)RNF216
Synonym gene name(s)TRIAD3, UBCE7IP1, ZIN
Protein nameE3 ubiquitin-protein ligase RNF216
Protein functionIsoform 1 acts as an E3 ubiquitin ligase, which accepts ubiquitin from specific E2 ubiquitin-conjugating enzymes, and then transfers it to substrates promoting their degradation by the proteasome. Promotes degradation of TRAF3, TLR4 and TLR9. Contributes to the regulation of antiviral responses. Down-regulates activation of NF-kappa-B, IRF3 activation and IFNB production. Isoform 3 inhibits TNF and IL-1 mediated activation of NF-kappa-B. Promotes TNF and RIP mediated apoptosis. {ECO:0000269|PubMed:15107846, ECO:0000269|PubMed:19893624}.
Subcellular locationCytoplasm.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: Q9NWF9
Gene Ontology
(Biological Process)
Complete annatation
apoptotic process [GO:0006915];
negative regulation of type I interferon production [GO:0032480];
proteasome-mediated ubiquitin-dependent protein catabolic process [GO:0043161];
protein K48-linked ubiquitination [GO:0070936];
protein linear polyubiquitination [GO:0097039];
regulation of defense response to virus by host [GO:0050691];
regulation of interferon-beta production [GO:0032648];
viral process [GO:0016032]
Gene Ontology
(Molecular Function)
Complete annatation
ligase activity [GO:0016874];
metal ion binding [GO:0046872];
ubiquitin protein ligase activity [GO:0061630]
Gene Ontology
(Cellular Component)
Complete annatation
cytosol [GO:0005829];
nucleus [GO:0005634]
Protein-protein interaction119981
Phylogenetic treeQ9NWF9
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD3-0.314896342554750.3381196935555740.458914463872706
AZA vs. DISU0.5911011074166280.02069063044205890.340120570647323
AZA vs. IL7-0.1041712904594890.7426633389692540.999311006273513
AZA vs. SAHA0.03703688756010630.8978364624852630.977360688612925
DISU vs. CD30.8919710125004860.01453066260575130.0395870609288081
DISU vs. IL7-0.703737376575470.00570638511685340.0814618898073532
DISU vs. SAHA-0.5524506986065970.05961309796122380.308027517720459
DMSO vs. AZA0.1154711530555270.7224594770576711
DMSO vs. CD30.4146271664475080.1968833737807910.295538299839723
DMSO vs. DISU-0.478542223571960.0600830301378120.472327211359847
DMSO vs. IL7-0.211769649326190.5148971360949630.887822730765496
DMSO vs. SAHA-0.0839330308638930.7781555065859820.93736560220614
HIV vs. Mock in Activation0.0009711751173417450.9987535732462730.999983755607037
HIV vs. Mock in Latency-0.125272435275480.4480412240888510.999834320637052
IL7 vs. CD30.2187143393785160.4970305998988180.627344015430822
SAHA vs. CD30.3268971014407680.3557231854157290.472271024950668
SAHA vs. IL70.1372336573402920.6308641002456520.816892769593663
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) TRUE
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK -0.648905 0.000305467
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
0.547 0.876 0.658 0.679 0.825
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

not found

Protein Secondary Structure       (annotations from PDB)      top

not found

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

HIV Partner Interaction Type PubMed
Vif binds 12600646
Vif interacts with 12600646
HIV-1 virus replication enhanced by expression of human gene 18854154

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
not found