Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0002514
UniProt IDQ6ZMZ0
Primary gene name(s)RNF19B
Synonym gene name(s)IBRDC3, NKLAM
Protein nameE3 ubiquitin-protein ligase RNF19B
Protein functionE3 ubiquitin-protein ligase which accepts ubiquitin from E2 ubiquitin-conjugating enzymes UBE2L3 and UBE2L6 in the form of a thioester and then directly transfers the ubiquitin to targeted substrates, such as UCKL1. Involved in the cytolytic activity of natural killer cells and cytotoxic T-cells. {ECO:0000269|PubMed:10438909, ECO:0000269|PubMed:16709802}.
Subcellular locationCytoplasmic granule membrane {ECO:0000269|PubMed:10438909};
Multi-pass membrane protein {ECO:0000269|PubMed:10438909}.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: Q6ZMZ0
Gene Ontology
(Biological Process)
Complete annatation
adaptive immune response [GO:0002250];
positive regulation of proteasomal ubiquitin-dependent protein catabolic process [GO:0032436];
protein polyubiquitination [GO:0000209];
protein ubiquitination involved in ubiquitin-dependent protein catabolic process [GO:0042787]
Gene Ontology
(Molecular Function)
Complete annatation
ligase activity [GO:0016874];
ubiquitin conjugating enzyme binding [GO:0031624];
ubiquitin protein ligase activity [GO:0061630];
ubiquitin-protein transferase activity [GO:0004842];
zinc ion binding [GO:0008270]
Gene Ontology
(Cellular Component)
Complete annatation
cytolytic granule [GO:0044194];
cytoplasm [GO:0005737];
cytosol [GO:0005829];
integral component of membrane [GO:0016021];
ubiquitin ligase complex [GO:0000151]
Protein-protein interaction126065
Phylogenetic treeQ6ZMZ0
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD30.7365439446428180.02673125461898080.0591186309049566
AZA vs. DISU0.4975696620184340.05073970367199130.504518663028758
AZA vs. IL7-0.006435642505049370.9736159120657790.999311006273513
AZA vs. SAHA-0.2969526512193390.2276581746839430.597566337856414
DISU vs. CD3-0.2518142153656050.4897810275545610.616634027341306
DISU vs. IL7-0.5134457220431390.04283246944290530.27378372450084
DISU vs. SAHA-0.7923065613261570.007133018431290440.0826936313676755
DMSO vs. AZA-0.1079183323885840.5263231845465551
DMSO vs. CD3-0.8561400107924810.008377014758899510.0214260997186454
DMSO vs. DISU-0.6075197526897680.01349368640350290.24295084121958
DMSO vs. IL70.1087531961527850.5504105088994280.897857969069977
DMSO vs. SAHA-0.194782649364940.4128559989310350.753878332534322
HIV vs. Mock in Activation0.3394777403530010.5860749725088520.999983755607037
HIV vs. Mock in Latency0.02765930158466590.8689093067739260.999834320637052
IL7 vs. CD3-0.7365007220611720.02323884316624940.0590172880096602
SAHA vs. CD3-1.057432902030880.00348133522066030.0104282152059773
SAHA vs. IL7-0.2932060288578080.2334706747815530.474003593632464
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock 0.963 8.67E-10 8.68E-08
Infected vs. Bystander 1.013 3.26E-10 2.23E-08
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) TRUE
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK 0.270911 0.0510603
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
unknown unknown unknown unknown unknown
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

not found

Protein Secondary Structure       (annotations from PDB)      top

not found

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

not found

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
not found