Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0002508
UniProt IDP50876
Primary gene name(s)RNF144A
Synonym gene name(s)KIAA0161, RNF144, UBCE7IP4
Protein nameE3 ubiquitin-protein ligase RNF144A
Protein functionE3 ubiquitin-protein ligase which accepts ubiquitin from E2 ubiquitin-conjugating enzymes UBE2L3 and UBE2L6 in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Mediates the ubiquitination and degradation of the DNA damage kinase PRKDC. {ECO:0000250, ECO:0000269|PubMed:24979766}.
Subcellular locationCell membrane {ECO:0000269|PubMed:24979766};
Single-pass membrane protein {ECO:0000305}. Cytoplasmic vesicle membrane {ECO:0000269|PubMed:24979766}.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: P50876
Gene Ontology
(Biological Process)
Complete annatation
positive regulation of proteasomal ubiquitin-dependent protein catabolic process [GO:0032436];
protein polyubiquitination [GO:0000209];
protein ubiquitination involved in ubiquitin-dependent protein catabolic process [GO:0042787]
Gene Ontology
(Molecular Function)
Complete annatation
ligase activity [GO:0016874];
ubiquitin conjugating enzyme binding [GO:0031624];
ubiquitin protein ligase activity [GO:0061630];
zinc ion binding [GO:0008270]
Gene Ontology
(Cellular Component)
Complete annatation
cytoplasmic vesicle membrane [GO:0030659];
Golgi apparatus [GO:0005794];
integral component of membrane [GO:0016021];
plasma membrane [GO:0005886];
ubiquitin ligase complex [GO:0000151]
Protein-protein interaction115125
Phylogenetic treeP50876
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD3-3.2166927200087200
AZA vs. DISU-0.08387739029889250.7395617963839150.974754980471983
AZA vs. IL70.0009540009281526750.9960260716438380.999311006273513
AZA vs. SAHA-0.4033893160546660.09974189283615120.399075835744187
DISU vs. CD33.120996584719662.1094237467878e-151.18647214772684e-13
DISU vs. IL70.0753344846838940.7642932374233540.944713066100119
DISU vs. SAHA-0.3176679622063470.2769084701949240.656590238248537
DMSO vs. AZA-0.0004366564728432750.9979100084243551
DMSO vs. CD33.2062854208423100
DMSO vs. DISU0.08210537374629710.7358105332964510.967658472195267
DMSO vs. IL70.008423642608567730.9624851578910520.993151606293804
DMSO vs. SAHA-0.4091380373540790.08378657909564020.337542778281314
HIV vs. Mock in Activation0.3863943881316830.5356073143384410.999983755607037
HIV vs. Mock in Latency-0.108243533931460.7754607758300020.999834320637052
IL7 vs. CD33.2238751132205700
SAHA vs. CD32.78984807333071.3855583347322e-134.87858974402201e-12
SAHA vs. IL7-0.4070638318232930.09534176741724410.279710475679471
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change 2.14877903
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) TRUE
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK 0.165099 0.289022
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
unknown unknown unknown unknown unknown
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
204040_at 1.72 No downregulated in CD8+ cells

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

not found

Protein Secondary Structure       (annotations from PDB)      top

PDB Accession Method Resolution Chain Structure Preview
1WIM NMR - A=20-100.

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

not found

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
not found