Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0002507
UniProt IDQ96EQ8
Primary gene name(s)RNF125
Synonym gene name(s)unknown
Protein nameE3 ubiquitin-protein ligase RNF125
Protein functionE3 ubiquitin-protein ligase that acts as a positive regulator of T-cell activation. E3 ligase proteins mediate ubiquitination and subsequent proteasomal degradation of target proteins. {ECO:0000269|PubMed:15843525}.
Subcellular locationGolgi apparatus membrane {ECO:0000269|PubMed:17990982};
Lipid-anchor {ECO:0000269|PubMed:17990982}. Note=Shows a reticular staining pattern within the cell and is probably expressed at other intracellular membranes in addition to the Golgi membrane. Not detected at the plasma membrane. {ECO:0000269|PubMed:17990982}.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: Q96EQ8
Gene Ontology
(Biological Process)
Complete annatation
adaptive immune response [GO:0002250];
positive regulation of proteasomal ubiquitin-dependent protein catabolic process [GO:0032436];
protein polyubiquitination [GO:0000209]
Gene Ontology
(Molecular Function)
Complete annatation
ligase activity [GO:0016874];
ubiquitin conjugating enzyme binding [GO:0031624];
ubiquitin protein ligase activity [GO:0061630];
zinc ion binding [GO:0008270]
Gene Ontology
(Cellular Component)
Complete annatation
intracellular [GO:0005622]
Protein-protein interaction120281
Phylogenetic treeQ96EQ8
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD3-0.6304388452479180.2050760713204940.310925112860123
AZA vs. DISU-0.3875974991348860.1259567979441180.688578819756184
AZA vs. IL70.138981313727040.469247504538880.999311006273513
AZA vs. SAHA-1.032736388162853.1982386474283e-050.0016177936030649
DISU vs. CD30.2308040074036990.6541224705272170.754014079737498
DISU vs. IL70.51744659924090.04075937679424980.266514004712171
DISU vs. SAHA-0.6442229305412430.03005121506297880.206804503294134
DMSO vs. AZA-0.04365955779334590.7940778843102361
DMSO vs. CD30.5753837747998130.2505411629035740.356142915563535
DMSO vs. DISU0.3423033222678450.1610715182159420.667942193285523
DMSO vs. IL70.1897221035355870.2909063271717160.777982395034921
DMSO vs. SAHA-0.995658232056883.29011247172106e-050.00147575253158655
HIV vs. Mock in Activation0.1769317009096570.86978444611280.999983755607037
HIV vs. Mock in Latency0.04479842387813870.7858542634341540.999834320637052
IL7 vs. CD30.7772381749334990.115741371025560.212172341034871
SAHA vs. CD3-0.4267855242967820.4018905504752210.518091582178065
SAHA vs. IL7-1.175192422473622.09735392475707e-068.52170163390515e-05
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) TRUE
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK -0.391638 0.00199466
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
unknown unknown unknown unknown unknown
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

not found

Protein Secondary Structure       (annotations from PDB)      top

PDB Accession Method Resolution Chain Structure Preview
5DKA X-ray 1.5Å A/B=1-232.

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

HIV Partner Interaction Type PubMed
Envelope surface glycoprotein gp120 downregulated by 17643463
Tat upregulates 22632162

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
hsa04622 RIG-I-like receptor signaling pathway - Homo sapiens (human)