Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0002490
UniProt IDP84095
Primary gene name(s)RHOG
Synonym gene name(s)ARHG
Protein nameRho-related GTP-binding protein RhoG
Protein functionRequired for the formation of membrane ruffles during macropinocytosis. Plays a role in cell migration and is required for the formation of cup-like structures during trans-endothelial migration of leukocytes. In case of Salmonella enterica infection, activated by SopB and ARHGEF26/SGEF, which induces cytoskeleton rearrangements and promotes bacterial entry. {ECO:0000269|PubMed:15133129, ECO:0000269|PubMed:17074883, ECO:0000269|PubMed:17875742, ECO:0000269|PubMed:20679435}.
Subcellular locationCell membrane {ECO:0000305};
Lipid-anchor {ECO:0000305};
Cytoplasmic side {ECO:0000305}.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: P84095
Gene Ontology
(Biological Process)
Complete annatation
actin cytoskeleton organization [GO:0030036];
activation of GTPase activity [GO:0090630];
cell chemotaxis [GO:0060326];
platelet activation [GO:0030168];
positive regulation of cell proliferation [GO:0008284];
positive regulation of establishment of protein localization to plasma membrane [GO:0090004];
positive regulation of transcription, DNA-templated [GO:0045893];
Rac protein signal transduction [GO:0016601];
regulation of ruffle assembly [GO:1900027];
regulation of small GTPase mediated signal transduction [GO:0051056];
Rho protein signal transduction [GO:0007266]
Gene Ontology
(Molecular Function)
Complete annatation
GTPase activity [GO:0003924];
GTP binding [GO:0005525]
Gene Ontology
(Cellular Component)
Complete annatation
cytosol [GO:0005829];
endoplasmic reticulum membrane [GO:0005789];
extracellular exosome [GO:0070062];
focal adhesion [GO:0005925];
plasma membrane [GO:0005886]
Protein-protein interaction106884
Phylogenetic treeP84095
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD31.008429398447140.002280808107208990.00741334790261597
AZA vs. DISU-0.02751990102076870.9134217124246250.994271142470137
AZA vs. IL7-0.08290792804582140.6674489650870430.999311006273513
AZA vs. SAHA-0.0554294325037150.8204572518048530.955836819978851
DISU vs. CD3-1.048051640297560.004220667363509460.0140913266528331
DISU vs. IL7-0.06529442465614910.7957124558017630.954423799099071
DISU vs. SAHA-0.02493420258582960.9318391292263770.984298990369098
DMSO vs. AZA0.02377835352699620.8875151878610521
DMSO vs. CD3-0.9980110629425070.001995081045318430.00622791047404104
DMSO vs. DISU0.04896386139222190.8410220129125580.982583769983649
DMSO vs. IL7-0.09908725928441650.5827696028434060.908008795786454
DMSO vs. SAHA-0.08313322146451580.7247222908166980.919204979591309
HIV vs. Mock in Activation0.1966703966378860.7527148532034910.999983755607037
HIV vs. Mock in Latency-0.0118857468771240.9428887951025580.999834320637052
IL7 vs. CD3-1.086484713621820.0008535464834168450.00370486838099494
SAHA vs. CD3-1.086290749606140.002342138606816420.00741046164281409
SAHA vs. IL70.02605847659351560.9150683163018080.967777997609868
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change -0.99687562
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) FALSE
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK -0.246228 0.0653889
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
1.047 0.951 0.881 0.886 0.887
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

not found

Protein Secondary Structure       (annotations from PDB)      top

not found

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

HIV Partner Interaction Type PubMed
Vpr upregulates 10713718

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
hsa05100 Bacterial invasion of epithelial cells - Homo sapiens (human)
hsa05131 Shigellosis - Homo sapiens (human)
hsa05132 Salmonella infection - Homo sapiens (human)