Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0002489
UniProt IDP62745
Primary gene name(s)RHOB
Synonym gene name(s)ARH6, ARHB
Protein nameRho-related GTP-binding protein RhoB
Protein functionMediates apoptosis in neoplastically transformed cells after DNA damage. Not essential for development but affects cell adhesion and growth factor signaling in transformed cells. Plays a negative role in tumorigenesis as deletion causes tumor formation. Involved in intracellular protein trafficking of a number of proteins. Targets PKN1 to endosomes and is involved in trafficking of the EGF receptor from late endosomes to lysosomes. Also required for stability and nuclear trafficking of AKT1/AKT which promotes endothelial cell survival during vascular development. Serves as a microtubule-dependent signal that is required for the myosin contractile ring formation during cell cycle cytokinesis. Required for genotoxic stress-induced cell death in breast cancer cells. {ECO:0000269|PubMed:10508588, ECO:0000269|PubMed:15226397, ECO:0000269|PubMed:16236794, ECO:0000269|PubMed:21373644, ECO:0000269|PubMed:9478917}.
Subcellular locationLate endosome membrane;
Lipid-anchor. Cell membrane;
Lipid-anchor. Nucleus. Cleavage furrow. Note=Late endosomal membrane, geranylgeranylated form. Plasma membrane, farnesylated form. Also detected at the nuclear margin and in the nucleus. Translocates to the equatorial region before furrow formation in a ECT2-dependent manner.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: P62745
Gene Ontology
(Biological Process)
Complete annatation
angiogenesis [GO:0001525];
apoptotic process [GO:0006915];
cell adhesion [GO:0007155];
cell differentiation [GO:0030154];
cellular response to hydrogen peroxide [GO:0070301];
cellular response to ionizing radiation [GO:0071479];
cytokinesis [GO:0000910];
endosome to lysosome transport [GO:0008333];
endothelial tube morphogenesis [GO:0061154];
intracellular protein transport [GO:0006886];
negative regulation of cell cycle [GO:0045786];
negative regulation of cell migration [GO:0030336];
platelet activation [GO:0030168];
positive regulation of angiogenesis [GO:0045766];
positive regulation of apoptotic process [GO:0043065];
positive regulation of endothelial cell migration [GO:0010595];
regulation of cell migration [GO:0030334];
regulation of small GTPase mediated signal transduction [GO:0051056];
Rho protein signal transduction [GO:0007266]
Gene Ontology
(Molecular Function)
Complete annatation
GDP binding [GO:0019003];
GTPase activity [GO:0003924];
GTP binding [GO:0005525]
Gene Ontology
(Cellular Component)
Complete annatation
cleavage furrow [GO:0032154];
cytosol [GO:0005829];
early endosome [GO:0005769];
endosome membrane [GO:0010008];
extracellular exosome [GO:0070062];
focal adhesion [GO:0005925];
late endosome membrane [GO:0031902];
nucleus [GO:0005634];
plasma membrane [GO:0005886]
Protein-protein interaction106881
Phylogenetic treeP62745
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD32.262768507581469.07719264420237e-077.10339810661569e-06
AZA vs. DISU0.2280742060748420.4036795547465680.905545478540778
AZA vs. IL7-0.4597230061161640.04127436084189850.61646999655158
AZA vs. SAHA0.04277082109958490.8727927207492250.970944344837768
DISU vs. CD3-2.049122334551325.05059374849015e-050.000311891233776078
DISU vs. IL7-0.6953220507254110.01288795702059030.137304559777667
DISU vs. SAHA-0.1831981894603760.552575734019370.85230466558243
DMSO vs. AZA-0.21454859270580.2902887825849051
DMSO vs. CD3-2.492492007296871.20807971293324e-071.05121452519475e-06
DMSO vs. DISU-0.4451222210859020.1075868891917540.581579633622478
DMSO vs. IL7-0.2350790839082380.2722553048715160.76921890271593
DMSO vs. SAHA0.2522241850843840.3302660479508230.684372282231108
HIV vs. Mock in Activation0.7052096251319640.4501847566686540.999983755607037
HIV vs. Mock in Latency-0.3426107057711710.4745243709912950.999834320637052
IL7 vs. CD3-2.716910834411991.88853586102766e-082.68040499371657e-07
SAHA vs. CD3-2.244256293680051.15123302901754e-069.18387566682233e-06
SAHA vs. IL70.4991319983123260.06345184307337310.21498608282491
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
-1.8 0.007127256 -1.8 0.003893317 -1.3 0.38539539
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) TRUE
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK 1.56035 0.000305467
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
unknown unknown unknown unknown unknown
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

Drugbank ID Drug Name Drug Status Pharmacological Action Drug Action
DB00083 Botulinum Toxin Type A approved, investigational unknown unknown

Protein Secondary Structure       (annotations from PDB)      top

PDB Accession Method Resolution Chain Structure Preview
2FV8 X-ray 1.9Å A=4-187.

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

HIV Partner Interaction Type PubMed
Vpr upregulates 10713718

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
not found