Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0002468
UniProt IDO95072
Primary gene name(s)REC8
Synonym gene name(s)REC8L1
Protein nameMeiotic recombination protein REC8 homolog
Protein functionRequired during meiosis for separation of sister chromatids and homologous chromosomes. Proteolytic cleavage of REC8 on chromosome arms by separin during anaphase I allows for homologous chromosome separation in meiosis I and cleavage of REC8 on centromeres during anaphase II allows for sister chromatid separation in meiosis II, By similarity. {ECO:0000250}.
Subcellular locationNucleus {ECO:0000250|UniProtKB:Q6AYJ4}. Chromosome {ECO:0000250|UniProtKB:Q6AYJ4}. Chromosome, centromere {ECO:0000250|UniProtKB:Q6AYJ4}. Note=In meiotic chromosomes, localized along axial elements in prophase from the leptotene to diplotene stages. At later prophase stages, diakinesis and metaphase I, localized along interstitial axes of chromosomes including both centromere and arm regions. No longer detected in arm regions in anaphase I but persists on centromere regions until metaphase II. Localised to centromeres and spindle poles in endopolyploid tumor cells. {ECO:0000250|UniProtKB:Q6AYJ4}.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: O95072
Gene Ontology
(Biological Process)
Complete annatation
double-strand break repair [GO:0006302];
double-strand break repair via homologous recombination [GO:0000724];
fertilization [GO:0009566];
male meiosis I [GO:0007141];
meiotic nuclear division [GO:0007126];
oocyte maturation [GO:0001556];
reciprocal meiotic recombination [GO:0007131];
seminiferous tubule development [GO:0072520];
sister chromatid cohesion [GO:0007062];
spermatid development [GO:0007286];
spermatogenesis [GO:0007283];
synaptonemal complex assembly [GO:0007130]
Gene Ontology
(Molecular Function)
Complete annatation
unknown
Gene Ontology
(Cellular Component)
Complete annatation
condensed nuclear chromosome kinetochore [GO:0000778];
lateral element [GO:0000800];
male germ cell nucleus [GO:0001673];
nuclear meiotic cohesin complex [GO:0034991];
nucleus [GO:0005634]
Protein-protein interaction115306
Phylogenetic treeO95072
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
      unknown
Brass et al., Science, 2008
      unknown
Smith et al., J. Immunol, 2010
      unknown
Interferon-stimulated
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model


DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD3-0.3990722164170870.3138934267372810.433321279335437
AZA vs. DISU0.2618649785818990.3107031910218910.866359582216792
AZA vs. IL70.42798212632490.0420693460059770.621677698380482
AZA vs. SAHA-0.5518696445801540.02860943538768780.193747273359803
DISU vs. CD30.6478668117220990.1212032776874080.216779569038917
DISU vs. IL70.1562863161734190.5407645672052020.84908334260775
DISU vs. SAHA-0.8111991159191570.006867531646783220.0804200228816394
DMSO vs. AZA0.183517701079740.2962048464694781
DMSO vs. CD30.5690947944771390.1387044944731950.223031615886293
DMSO vs. DISU-0.08073168078794360.7454387397520390.969482258605607
DMSO vs. IL70.2529039182606390.1759179694234780.68633407932787
DMSO vs. SAHA-0.7400261944249350.002365067108204610.032921515168061
HIV vs. Mock in Activation0.3067635258672080.7160345906842440.999983755607037
HIV vs. Mock in Latency0.04570645943082010.7874075003153640.999834320637052
IL7 vs. CD30.8313164585460210.04802486658295070.106156153982228
SAHA vs. CD3-0.176736663827280.6624014218126780.748810248797319
SAHA vs. IL7-0.982486690512249.83451561520354e-050.0019327964587168
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) TRUE
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK 0.568743 0.558961
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
unknown unknown unknown unknown unknown
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
218599_at 1.46 No upregulated in CD8+ cells

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

not found

Protein Secondary Structure       (annotations from PDB)      top

not found

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

not found

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
hsa04114 Oocyte meiosis - Homo sapiens (human)
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