Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0002463
UniProt IDP18754
Primary gene name(s)RCC1
Synonym gene name(s)CHC1
Protein nameRegulator of chromosome condensation
Protein functionGuanine-nucleotide releasing factor that promotes the exchange of Ran-bound GDP by GTP. Involved in the regulation of onset of chromosome condensation in the S phase. Binds both to the nucleosomes and double-stranded DNA. RCC1-Ran complex, together with other proteins acts as a component of a signal transmission pathway that detects unreplicated DNA. Plays a key role in nucleo-cytoplasmic transport, mitosis and nuclear-envelope assembly. {ECO:0000269|PubMed:1944575}.
Subcellular locationNucleus {ECO:0000269|PubMed:17435751}. Cytoplasm {ECO:0000269|PubMed:17435751}. Note=Becomes dispersed throughout the cytoplasm during mitosis.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: P18754
Gene Ontology
(Biological Process)
Complete annatation
cell division [GO:0051301];
chromosome segregation [GO:0007059];
G1/S transition of mitotic cell cycle [GO:0000082];
mitotic nuclear division [GO:0007067];
mitotic spindle organization [GO:0007052];
regulation of mitotic nuclear division [GO:0007088];
spindle assembly [GO:0051225];
viral process [GO:0016032]
Gene Ontology
(Molecular Function)
Complete annatation
chromatin binding [GO:0003682];
histone binding [GO:0042393];
nucleosomal DNA binding [GO:0031492];
Ran guanyl-nucleotide exchange factor activity [GO:0005087]
Gene Ontology
(Cellular Component)
Complete annatation
condensed nuclear chromosome [GO:0000794];
cytoplasm [GO:0005737];
nuclear chromatin [GO:0000790];
nuclear membrane [GO:0031965];
nucleoplasm [GO:0005654];
nucleus [GO:0005634]
Protein-protein interaction107529
Phylogenetic treeP18754
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD32.104717742717717.00913216356014e-101.0438897230156e-08
AZA vs. DISU0.5575652675447570.03015646236156670.411332385591532
AZA vs. IL70.4510365104882550.02373547971812790.491596466916618
AZA vs. SAHA-0.2921608729083840.2337381800126260.605078022584633
DISU vs. CD3-1.560015795952812.67956062182106e-050.000177156564970749
DISU vs. IL7-0.1147785976571660.6511420182840830.898768510225857
DISU vs. SAHA-0.8490276743779460.004514860135538260.0600560080341545
DMSO vs. AZA0.001016497459849670.9957522361394461
DMSO vs. CD3-2.118986597644751.99987248983291e-102.98924849875242e-09
DMSO vs. DISU-0.5594551839144220.02367174720894360.315201359349298
DMSO vs. IL70.4578232343542740.01146068672713670.224085411688179
DMSO vs. SAHA-0.2996729336806190.206701263152140.547106897474529
HIV vs. Mock in Activation0.05882444638707070.9246159049480470.999983755607037
HIV vs. Mock in Latency-0.2950129151239570.07759718971406170.999834320637052
IL7 vs. CD3-1.644489725036225.89158197672468e-076.05342979789851e-06
SAHA vs. CD3-2.422601074515628.8438700807103e-111.92041962703608e-09
SAHA vs. IL7-0.7478531882808260.002394176945050840.0224718700309442
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) FALSE
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK 0.785661 0.000305467
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
1.002 0.959 0.934 0.904 0.856
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

not found

Protein Secondary Structure       (annotations from PDB)      top

PDB Accession Method Resolution Chain Structure Preview
1A12 X-ray 1.7Å A/B/C=9-421.
1I2M X-ray 1.7Å B/D=20-421.
5E1B X-ray 1.6Å D/E=2-7.
5E1D X-ray 1.4Å D/E=3-7.
5E1M X-ray 1.7Å D/E=3-7.
5E1O X-ray 2.0Å D/E=3-7.
5E2A X-ray 1.7Å D/E=3-7.
5E2B X-ray 1.9Å D/E=3-7.

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

HIV Partner Interaction Type PubMed
retropepsin cleaves 22944692

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
not found