Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0002461
UniProt IDQ9HBD1
Primary gene name(s)RC3H2
Synonym gene name(s)MNAB, RNF164
Protein nameRoquin-2
Protein functionPost-transcriptional repressor of mRNAs containing a conserved stem loop motif, called constitutive decay element, CDE, which is often located in the 3'-UTR, as in HMGXB3, ICOS, IER3, NFKBID, NFKBIZ, PPP1R10, TNF and in many more mRNAs. Binds to CDE and promotes mRNA deadenylation and degradation. This process does not involve miRNAs. In follicular helper T, Tfh cells, represses of ICOS and TNFRSF4 expression, thus preventing spontaneous Tfh cell differentiation, germinal center B-cell differentiation in the absence of immunization and autoimmunity. In resting or LPS-stimulated macrophages, controls inflammation by suppressing TNF expression. Also recognizes CDE in its own mRNA and in that of paralogous RC3H2, possibly leading to feedback loop regulation, By similarity. May act as a ubiquitin E3 ligase. Involved in the ubiquitination of MAP3K5, PubMed:24448648. {ECO:0000250|UniProtKB:P0C090, ECO:0000269|PubMed:24448648}.
Subcellular locationCytoplasm, P-body {ECO:0000250}. Note=During stress, such as that induced by arsenite, localizes to cytosolic stress granules. Localization to stress granules, but not to P-bodies, depends upon the RING-type zinc finger. {ECO:0000269|PubMed:20412057}.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: Q9HBD1
Gene Ontology
(Biological Process)
Complete annatation
B cell homeostasis [GO:0001782];
limb development [GO:0060173];
lung alveolus development [GO:0048286];
lymph node development [GO:0048535];
multicellular organism growth [GO:0035264];
positive regulation of NIK/NF-kappaB signaling [GO:1901224];
post-embryonic development [GO:0009791];
posttranscriptional regulation of gene expression [GO:0010608];
protein polyubiquitination [GO:0000209];
spleen development [GO:0048536];
T cell homeostasis [GO:0043029];
T cell proliferation [GO:0042098];
T follicular helper cell differentiation [GO:0061470]
Gene Ontology
(Molecular Function)
Complete annatation
DNA binding [GO:0003677];
ligase activity [GO:0016874];
mRNA binding [GO:0003729];
poly(A RNA binding [GO:0044822];
ubiquitin-protein transferase activity [GO:0004842];
zinc ion binding [GO:0008270]
Gene Ontology
(Cellular Component)
Complete annatation
cell surface [GO:0009986];
cytoplasm [GO:0005737];
cytoplasmic mRNA processing body [GO:0000932];
membrane [GO:0016020];
nucleoplasm [GO:0005654]
Protein-protein interaction120029
Phylogenetic treeQ9HBD1
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD3-0.09468359331843580.7726804676516870.842741154095537
AZA vs. DISU0.5063372328423060.04778340527798140.493685436024875
AZA vs. IL70.0564167841728460.7691097225574470.999311006273513
AZA vs. SAHA0.204829887773520.4015427407711450.76021794453457
DISU vs. CD30.5883280642557650.1127384678435470.204749357141401
DISU vs. IL7-0.4585931082813560.07164175640665030.356006963027397
DISU vs. SAHA-0.3008285113373740.3106680475827690.69364120668443
DMSO vs. AZA-0.09321029587976460.5780666700789761
DMSO vs. CD3-0.009857974563027750.975413738705580.982916921311007
DMSO vs. DISU-0.6014010991075820.01506876835946310.256884999069128
DMSO vs. IL70.156924115499910.3828736138867640.835526886123764
DMSO vs. SAHA0.2905971162774130.2181080504509620.561440344358693
HIV vs. Mock in Activation0.1063633013884070.8654630772622220.999983755607037
HIV vs. Mock in Latency0.05254558380510.749982828019360.999834320637052
IL7 vs. CD30.1594317286909240.6202413567082860.733937664442141
SAHA vs. CD30.2737486473761470.4381700825933470.551315969496564
SAHA vs. IL70.1442921538475630.5538582039365160.765739573157759
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) TRUE
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK 0.213656 0.340112
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
unknown unknown unknown unknown unknown
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

not found

Protein Secondary Structure       (annotations from PDB)      top

PDB Accession Method Resolution Chain Structure Preview
4Z30 X-ray 2.7Å A=86-404.
4Z31 X-ray 2.5Å A/B=87-404.
4ZLC X-ray 2.7Å A/B/C/D=171-325.
4ZLD X-ray 1.6Å A=171-325.

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

not found

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
not found