Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0002445
UniProt IDQ8WWW0
Primary gene name(s)RASSF5
Synonym gene name(s)NORE1, RAPL
Protein nameRas association domain-containing protein 5
Protein functionPotential tumor suppressor. Seems to be involved in lymphocyte adhesion by linking RAP1A activation upon T-cell receptor or chemokine stimulation to integrin activation. Isoform 2 stimulates lymphocyte polarization and the patch-like distribution of ITGAL/LFA-1, resulting in an enhanced adhesion to ICAM1. Together with RAP1A may participate in regulation of microtubule growth. The association of isoform 2 with activated RAP1A is required for directional movement of endothelial cells during wound healing. May be involved in regulation of Ras apoptotic function. The RASSF5-STK4/MST1 complex may mediate HRAS and KRAS induced apoptosis. {ECO:0000269|PubMed:12676952, ECO:0000269|PubMed:12845325, ECO:0000269|PubMed:15569673}.
Subcellular locationCytoplasm. Cytoplasm, cytoskeleton. Note=Isoform 2 is mainly located in the perinuclear region of unstimulated primary T-cells. Upon stimulation translocates to the leading edge and colocalizes with ITGAL/LFA-1 in the peripheral zone of the immunological synapse. Isoform 2 is localized to growing microtubules in vascular endothelial cells and is dissociated from microtubules by activated RAP1A.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: Q8WWW0
Gene Ontology
(Biological Process)
Complete annatation
apoptotic process [GO:0006915];
intracellular signal transduction [GO:0035556];
negative regulation of cell proliferation [GO:0008285];
positive regulation of protein ubiquitination [GO:0031398];
regulation of apoptotic process [GO:0042981];
regulation of protein localization to nucleus [GO:1900180]
Gene Ontology
(Molecular Function)
Complete annatation
metal ion binding [GO:0046872]
Gene Ontology
(Cellular Component)
Complete annatation
cytoplasm [GO:0005737];
microtubule [GO:0005874];
nucleus [GO:0005634]
Protein-protein interaction123689
Phylogenetic treeQ8WWW0
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD3-0.6921247092583220.03510400637889930.0741746227593958
AZA vs. DISU-0.2101740952661010.4050193839816950.905959666736916
AZA vs. IL70.1995069691627750.2979639185802350.999311006273513
AZA vs. SAHA-0.177312847731120.4664977879930830.803395680629882
DISU vs. CD30.4698773289013830.1950473640928950.312797594071102
DISU vs. IL70.4004393995513570.1116708954210910.437365583564967
DISU vs. SAHA0.03490401162137420.9044895127284430.977138612944312
DMSO vs. AZA0.02534220498832620.8792150253652011
DMSO vs. CD30.7050986864917640.02791941814315080.059140331035794
DMSO vs. DISU0.2334410157073430.3376922903131110.837145111953607
DMSO vs. IL70.1815838954289940.31089405960480.793129486257956
DMSO vs. SAHA-0.2081321386920860.3763301104962850.724398272360826
HIV vs. Mock in Activation0.105938582436980.8646853301080440.999983755607037
HIV vs. Mock in Latency-0.0308628721386820.8513773557390250.999834320637052
IL7 vs. CD30.8984377918598470.005424142867068380.0179372763131465
SAHA vs. CD30.4913498762239370.1644269405288310.258925182212068
SAHA vs. IL7-0.3792478877860470.1188869501607270.31883875815259
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) FALSE
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK -0.361602 0.00657227
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
1.045 1.334 1.564 1.659 1.484
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

not found

Protein Secondary Structure       (annotations from PDB)      top

PDB Accession Method Resolution Chain Structure Preview
4LGD X-ray 3.0Å E/F/G/H=365-413.
4OH8 X-ray 2.2Å B=366-418.

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

not found

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
hsa04014 Ras signaling pathway - Homo sapiens (human)
hsa04015 Rap1 signaling pathway - Homo sapiens (human)
hsa04670 Leukocyte transendothelial migration - Homo sapiens (human)
hsa05200 Pathways in cancer - Homo sapiens (human)
hsa05223 Non-small cell lung cancer - Homo sapiens (human)