Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0002443
UniProt IDO95267
Primary gene name(s)RASGRP1
Synonym gene name(s)RASGRP
Protein nameRAS guanyl-releasing protein 1
Protein functionFunctions as a calcium- and diacylglycerol, DAG-regulated nucleotide exchange factor specifically activating Ras through the exchange of bound GDP for GTP, PubMed:15899849, PubMed:23908768. Activates the Erk/MAP kinase cascade, PubMed:15899849. Regulates T-cell/B-cell development, homeostasis and differentiation by coupling T-lymphocyte/B-lymphocyte antigen receptors to Ras, PubMed:10807788, PubMed:12839994. Regulates NK cell cytotoxicity and ITAM-dependent cytokine production by activation of Ras-mediated ERK and JNK pathways, PubMed:19933860. Functions in mast cell degranulation and cytokine secretion, regulating FcERI-evoked allergic responses, By similarity. May also function in differentiation of other cell types, PubMed:12845332. {ECO:0000250|UniProtKB:Q9Z1S3, ECO:0000269|PubMed:10807788, ECO:0000269|PubMed:12782630, ECO:0000269|PubMed:12839994, ECO:0000269|PubMed:12845332, ECO:0000269|PubMed:15060167, ECO:0000269|PubMed:15184873, ECO:0000269|PubMed:15899849, ECO:0000269|PubMed:19933860, ECO:0000269|PubMed:23908768}.
Subcellular locationCytoplasm, cytosol. Cell membrane;
Peripheral membrane protein. Golgi apparatus membrane;
Peripheral membrane protein. Endoplasmic reticulum membrane;
Peripheral membrane protein. Note=Found both in the cytosol and associated with membranes. Relocalization to the cell membrane upon activation is F-actin-dependent. Translocates to the Golgi in response to phorbol ester or nerve growth factor. Localizes to somata and dendrites but not to axons of hippocampal pyramidal cells, By similarity. {ECO:0000250}.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: O95267
Gene Ontology
(Biological Process)
Complete annatation
activation of GTPase activity [GO:0090630];
cell differentiation [GO:0030154];
cytokine production [GO:0001816];
inflammatory response to antigenic stimulus [GO:0002437];
MAPK cascade [GO:0000165];
mast cell degranulation [GO:0043303];
positive regulation of ERK1 and ERK2 cascade [GO:0070374];
positive regulation of granulocyte macrophage colony-stimulating factor production [GO:0032725];
positive regulation of GTPase activity [GO:0043547];
positive regulation of interferon-gamma secretion [GO:1902715];
positive regulation of JNK cascade [GO:0046330];
positive regulation of MAP kinase activity [GO:0043406];
positive regulation of natural killer cell differentiation [GO:0032825];
positive regulation of natural killer cell mediated cytotoxicity [GO:0045954];
positive regulation of protein phosphorylation [GO:0001934];
positive regulation of Ras protein signal transduction [GO:0046579];
positive regulation of T cell differentiation in thymus [GO:0033089];
positive regulation of tumor necrosis factor production [GO:0032760];
protein oligomerization [GO:0051259];
Ras protein signal transduction [GO:0007265];
regulation of ERK1 and ERK2 cascade [GO:0070372];
regulation of phosphatidylinositol 3-kinase signaling [GO:0014066];
secretory granule localization [GO:0032252];
signal transduction [GO:0007165];
vesicle transport along microtubule [GO:0047496]
Gene Ontology
(Molecular Function)
Complete annatation
calcium ion binding [GO:0005509];
diacylglycerol binding [GO:0019992];
guanyl-nucleotide exchange factor activity [GO:0005085];
lipid binding [GO:0008289];
phosphatidylcholine binding [GO:0031210];
phosphatidylserine binding [GO:0001786];
protein homodimerization activity [GO:0042803];
Ras guanyl-nucleotide exchange factor activity [GO:0005088];
zinc ion binding [GO:0008270]
Gene Ontology
(Cellular Component)
Complete annatation
cytosol [GO:0005829];
endoplasmic reticulum membrane [GO:0005789];
Golgi apparatus [GO:0005794];
Golgi membrane [GO:0000139];
mast cell granule [GO:0042629];
membrane [GO:0016020];
plasma membrane [GO:0005886]
Protein-protein interaction115429
Phylogenetic treeO95267
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD3-1.826161548650266.15636500667094e-086.20245543987596e-07
AZA vs. DISU-0.0008766747404746870.9972278504338320.999297613676408
AZA vs. IL70.06646433896810370.7287386569822130.999311006273513
AZA vs. SAHA-0.3970492436477710.1056862660552520.41347385790731
DISU vs. CD31.812587029736131.20892476973555e-061.13824684440935e-05
DISU vs. IL70.05835469598225760.8165380455839990.960983327332836
DISU vs. SAHA-0.3948940420502990.1794516388299810.545717469533298
DMSO vs. AZA-0.07755224826320920.6420680189917561
DMSO vs. CD31.737774874868471.22043602113209e-071.06031537822116e-06
DMSO vs. DISU-0.0783206346880510.7476978600102960.969690770254317
DMSO vs. IL70.1511630257330280.3990732974418810.8449480279405
DMSO vs. SAHA-0.3264070226052940.1684298836746880.495976314353503
HIV vs. Mock in Activation0.2404599721202850.69957327580150.999983755607037
HIV vs. Mock in Latency0.07233829842095780.6613631247717760.999834320637052
IL7 vs. CD31.900285072191631.00039088124504e-081.49443922229995e-07
SAHA vs. CD31.404250474666729.94100551500177e-050.000480092369186197
SAHA vs. IL7-0.467097947413670.05611729948970030.198851004350668
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) TRUE
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK -0.121148 0.4357
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
unknown unknown unknown unknown unknown
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

not found

Protein Secondary Structure       (annotations from PDB)      top

PDB Accession Method Resolution Chain Structure Preview
4L9M X-ray 3.0Å A=50-607.
4L9U X-ray 1.6Å A/B=739-793.

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

not found

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
hsa04010 MAPK signaling pathway - Homo sapiens (human)
hsa04014 Ras signaling pathway - Homo sapiens (human)
hsa04611 Platelet activation - Homo sapiens (human)
hsa04660 T cell receptor signaling pathway - Homo sapiens (human)
hsa05200 Pathways in cancer - Homo sapiens (human)