Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0002417
UniProt IDP61006
Primary gene name(s)RAB8A
Synonym gene name(s)MEL, RAB8
Protein nameRas-related protein Rab-8A
Protein functionThe small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different sets of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion. That Rab is involved in polarized vesicular trafficking and neurotransmitter release. Together with RAB11A, RAB3IP, the exocyst complex, PARD3, PRKCI, ANXA2, CDC42 and DNMBP promotes transcytosis of PODXL to the apical membrane initiation sites, AMIS, apical surface formation and lumenogenesis. Together with MYO5B and RAB11A participates in epithelial cell polarization. Plays an important role in ciliogenesis. Together with MICALL2, may also regulate adherens junction assembly. May play a role in insulin-induced transport to the plasma membrane of the glucose transporter GLUT4 and therefore play a role in glucose homeostasis. {ECO:0000269|PubMed:20890297, ECO:0000269|PubMed:21282656, ECO:0000269|PubMed:21844891}.
Subcellular locationCell membrane {ECO:0000305|PubMed:12221131};
Lipid-anchor {ECO:0000305|PubMed:12221131};
Cytoplasmic side {ECO:0000305|PubMed:12221131}. Golgi apparatus {ECO:0000269|PubMed:15837803}. Recycling endosome membrane {ECO:0000269|PubMed:19864458}. Cell projection, cilium {ECO:0000269|PubMed:21844891}. Cytoplasmic vesicle, phagosome {ECO:0000269|PubMed:21255211}. Cytoplasmic vesicle, phagosome membrane {ECO:0000250|UniProtKB:Q92930};
Lipid-anchor {ECO:0000250|UniProtKB:Q92930};
Cytoplasmic side {ECO:0000250|UniProtKB:Q92930}. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome, centriole {ECO:0000250|UniProtKB:P55258}. Cytoplasm, cytoskeleton, cilium basal body {ECO:0000250|UniProtKB:P55258}. Note=Colocalizes with OPTN at the Golgi complex and in vesicular structures close to the plasma membrane, PubMed:15837803. In the GDP-bound form, present in the perinuclear region, PubMed:12221131. Shows a polarized distribution to distal regions of cell protrusions in the GTP-bound form, PubMed:12221131. Colocalizes with PARD3, PRKCI, EXOC5, OCLN, PODXL and RAB11A in apical membrane initiation sites, AMIS during the generation of apical surface and lumenogenesis, PubMed:20890297. Localizes to tubular recycling endosome, PubMed:19864458. Recruited to phagosomes containing S.aureus or M.tuberculosis, PubMed:21255211. {ECO:0000269|PubMed:12221131, ECO:0000269|PubMed:15837803, ECO:0000269|PubMed:19864458, ECO:0000269|PubMed:20890297, ECO:0000269|PubMed:21255211}.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: P61006
Gene Ontology
(Biological Process)
Complete annatation
axonogenesis [GO:0007409];
cellular response to insulin stimulus [GO:0032869];
cilium assembly [GO:0060271];
G2/M transition of mitotic cell cycle [GO:0000086];
Golgi vesicle fusion to target membrane [GO:0048210];
membrane organization [GO:0061024];
protein localization to plasma membrane [GO:0072659];
protein secretion [GO:0009306];
regulation of long-term neuronal synaptic plasticity [GO:0048169];
regulation of protein transport [GO:0051223];
small GTPase mediated signal transduction [GO:0007264];
vesicle docking involved in exocytosis [GO:0006904]
Gene Ontology
(Molecular Function)
Complete annatation
GDP binding [GO:0019003];
GTPase activity [GO:0003924];
GTP binding [GO:0005525];
myosin V binding [GO:0031489];
Rab GTPase binding [GO:0017137]
Gene Ontology
(Cellular Component)
Complete annatation
centriole [GO:0005814];
centrosome [GO:0005813];
ciliary basal body [GO:0036064];
ciliary base [GO:0097546];
cilium [GO:0005929];
cytoplasmic vesicle membrane [GO:0030659];
cytosol [GO:0005829];
dendrite [GO:0030425];
extracellular exosome [GO:0070062];
Golgi membrane [GO:0000139];
neuronal cell body [GO:0043025];
nonmotile primary cilium [GO:0031513];
nucleolus [GO:0005730];
nucleus [GO:0005634];
phagocytic vesicle [GO:0045335];
phagocytic vesicle membrane [GO:0030670];
plasma membrane [GO:0005886];
postsynaptic density [GO:0014069];
primary cilium [GO:0072372];
recycling endosome membrane [GO:0055038];
synaptic vesicle [GO:0008021];
trans-Golgi network transport vesicle [GO:0030140]
Protein-protein interaction110382
Phylogenetic treeP61006
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
      Yes - Two siRNA pools inhibit HIV replication and inhibition of Tat-mediated transactivation of the HIV LTR is not observed
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD30.758183320704880.02305537479823690.0523565781164724
AZA vs. DISU-0.08126293793305540.7481298819603770.976008954784031
AZA vs. IL70.1545258879464650.422454061013880.999311006273513
AZA vs. SAHA-0.1131060424112560.6435527167021690.889611842976484
DISU vs. CD3-0.8507368965282340.02113972044486690.053891450361225
DISU vs. IL70.2262589249531240.3694196565206370.744446406382166
DISU vs. SAHA-0.03020111173212670.9175676748360960.98132056122989
DMSO vs. AZA-0.08464507774305940.6145266402513521
DMSO vs. CD3-0.851876308628780.00885741030187270.0224906263606459
DMSO vs. DISU-0.004732849483771570.9845304031451190.997435138456314
DMSO vs. IL70.2462597615792450.1718038118884690.68264430801014
DMSO vs. SAHA-0.03531810643599520.8812268685440.970940980432627
HIV vs. Mock in Activation0.1265388578839560.8387601556748510.999983755607037
HIV vs. Mock in Latency-0.002849086086897210.9862895878682970.999834320637052
IL7 vs. CD3-0.5968875321632570.06635944230116360.137925520381335
SAHA vs. CD3-0.8952621279545620.01384790503940960.0337538349480358
SAHA vs. IL7-0.270433565304960.2686563586353390.515289810403991
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) FALSE
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK 0.205726 0.236437
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
0.123 7.97E-04

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
1.021 1.101 1.093 1.149 1.106
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

not found

Protein Secondary Structure       (annotations from PDB)      top

PDB Accession Method Resolution Chain Structure Preview
3QBT X-ray 2.0Å A/C/E/G=6-176.
3TNF X-ray 2.5Å A=6-176.
4LHV X-ray 1.9Å A/B/C/D/E=6-176.
4LHW X-ray 1.5Å A/B/C/D/E=6-176.
4LHX X-ray 3.0Å A/B=1-184.
4LHY X-ray 3.1Å A/B=1-184.
4LHZ X-ray 3.2Å A/B=1-184.
4LI0 X-ray 3.3Å A/B=1-184.
5SZI X-ray 2.8Å A=1-207.

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

HIV Partner Interaction Type PubMed
Envelope surface glycoprotein gp120 complexes with 23125841
Gag-Pol complexes with 23125841
Pr55(Gag) complexes with 23125841
HIV-1 virus replication enhanced by expression of human gene 18976975
Nef complexes with 23125841

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
hsa04144 Endocytosis - Homo sapiens (human)
hsa04152 AMPK signaling pathway - Homo sapiens (human)
hsa04530 Tight junction - Homo sapiens (human)
hsa04972 Pancreatic secretion - Homo sapiens (human)